LOCUS       V020762                 5345 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020762
VERSION     V020762
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             58..1332
                     /label="SMAD3(NM_005902)"
                     /note="SMAD3(NM_005902)"
                     /gene="SMAD3"
     CDS             1390..1641
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
                     /label="VC155"
     polyA_signal    1656..1790
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1901..1917)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    1925..1941
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1949..1979)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1994..2015
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2303..2891)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3062..3922)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3923..4027)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4501..4517
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        4544..4847
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        4848..5051
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          5189..5285
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattcccatg
       61 tcgtccatcc tgcctttcac tcccccgatc gtgaagcgcc tgctgggctg gaagaagggc
      121 gagcagaacg ggcaggagga gaaatggtgc gagaaggcgg tcaagagcct ggtcaagaaa
      181 ctcaagaaga cggggcagct ggacgagctg gagaaggcca tcaccacgca gaacgtcaac
      241 accaagtgca tcaccatccc caggtccctg gatggccggt tgcaggtgtc ccatcggaag
      301 gggctccctc atgtcatcta ctgccgcctg tggcgatggc cagacctgca cagccaccac
      361 gagctacggg ccatggagct gtgtgagttc gccttcaata tgaagaagga cgaggtctgc
      421 gtgaatccct accactacca gagagtagag acaccagttc tacctcctgt gttggtgcca
      481 cgccacacag agatcccggc cgagttcccc ccactggacg actacagcca ttccatcccc
      541 gaaaacacta acttccccgc aggcatcgag ccccagagca atattccaga gaccccaccc
      601 cctggctacc tgagtgaaga tggagaaacc agtgaccacc agatgaacca cagcatggac
      661 gcaggttctc caaacctatc cccgaatccg atgtccccag cacataataa cttggacctg
      721 cagccagtta cctactgcga gccggccttc tggtgctcca tctcctacta cgagctgaac
      781 cagcgcgtcg gggagacatt ccacgcctcg cagccatcca tgactgtgga tggcttcacc
      841 gacccctcca attcggagcg cttctgccta gggctgctct ccaatgtcaa caggaatgca
      901 gcagtggagc tgacacggag acacatcgga agaggcgtgc ggctctacta catcggaggg
      961 gaggtcttcg cagagtgcct cagtgacagc gctatttttg tccagtctcc caactgtaac
     1021 cagcgctatg gctggcaccc ggccaccgtc tgcaagatcc caccaggatg caacctgaag
     1081 atcttcaaca accaggagtt cgctgccctc ctggcccagt cggtcaacca gggctttgag
     1141 gctgtctacc agttgacccg aatgtgcacc atccgcatga gcttcgtcaa aggctgggga
     1201 gcggagtaca ggagacagac tgtgaccagt accccctgct ggattgagct gcacctgaat
     1261 gggcctttgc agtggcttga caaggtcctc acccagatgg gctccccaag catccgctgt
     1321 tccagtgtgt ctggtacccg tccggcgtgc aaaatcccga acgacctgaa acagaaagtc
     1381 atgaaccacg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     1441 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1501 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaaactgag caaagacccc
     1561 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1621 ggcatggacg agctgtacaa gtaagcggcc gcggggatcc agacatgata agatacattg
     1681 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1741 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     1801 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tcggatcctc
     1861 tagagtcgat ctgcaggcat gctagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2041 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2101 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2701 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     3061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     3121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     3181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     3241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     3301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     3361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     3421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     3481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     3541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     3601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     3661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     4021 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     4081 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4141 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4201 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4261 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     4321 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     4381 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     4441 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     4501 gtaaaacgac ggccagtgag ttcgagcttg catgcctgca ggtcgttaca taacttacgg
     4561 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     4621 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     4681 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     4741 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     4801 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     4861 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4921 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4981 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     5041 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     5101 acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat ccggtactag
     5161 aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg
     5221 tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact
     5281 tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcgggc
     5341 ccacc
//
