LOCUS       V019831                 5079 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V019831
VERSION     V019831
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             883..2193
                     /label="SEPTIN7(NM_001788)"
                     /note="SEPTIN7(NM_001788)"
                     /gene="SEPTIN7"
     polyA_signal    2219..2353
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2463..2479)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2487..2503
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2511..2541)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2556..2577
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2865..3453)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3624..4484)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4485..4589)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5063..5079
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttatgtcggt cagtgcgaga
      901 tccgctgctg ctgaggagag gagcgtcaac agcagcacca tggtagctca acagaagaac
      961 cttgaaggct atgtgggatt tgccaatctc ccaaatcaag tatacagaaa atcggtgaag
     1021 agaggttttg aattcacgct tatggtagtg ggtgaatctg gattgggaaa gtcgacatta
     1081 atcaactcat tattcctcac agatttgtat tctccagagt atccaggtcc ttctcataga
     1141 attaaaaaga ctgtacaggt ggaacaatcc aaagttttaa tcaaagaagg tggtgttcag
     1201 ttgctgctca caatagttga taccccagga tttggagatg cagtggataa tagtaattgc
     1261 tggcagcctg ttatcgacta cattgatagt aaatttgagg actacctaaa tgcagaatca
     1321 cgagtgaaca gacgtcagat gcctgataac agggtgcagt gttgtttata cttcattgct
     1381 ccttcaggac atggacttaa accattggat attgagttta tgaagcgttt gcatgaaaaa
     1441 gtgaatatca tcccacttat tgccaaagca gacacactca caccagagga atgccaacag
     1501 tttaaaaaac agataatgaa agaaatccaa gaacataaaa ttaaaatata cgaatttcca
     1561 gaaacagatg atgaagaaga aaataaactt gttaaaaaga taaaggaccg tttacctctt
     1621 gctgtggtag gtagtaatac tatcattgaa gttaatggca aaagggtcag aggaaggcag
     1681 tatccttggg gtgttgctga agttgaaaat ggtgaacatt gtgattttac aatcctaaga
     1741 aatatgttga taagaacaca catgcaggac ttgaaagatg ttactaataa tgtccactat
     1801 gagaactaca gaagcagaaa acttgcagct gtgacttata atggagttga taacaacaag
     1861 aataaagggc agctgactaa gagccctctg gcacaaatgg aagaagaaag aagggagcat
     1921 gtagctaaaa tgaagaagat ggagatggag atggagcagg tgtttgagat gaaggtcaaa
     1981 gaaaaagttc aaaaactgaa ggactctgaa gctgagctcc agcggcgcca tgagcaaatg
     2041 aaaaagaatt tggaagcaca gcacaaagaa ttggaggaaa aacgtcgtca gttcgaggat
     2101 gagaaagcaa actgggaagc tcaacaacgt attttagaac aacagaactc ttcaagaacc
     2161 ttggaaaaga acaagaagaa agggaagatc ttttaactcg aggtaccgcg gccgcgggga
     2221 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2281 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2341 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2401 gtgggaggtt tttcggatcc tctagagtcg atctgcaggc atgctagctt ggcgtaatca
     2461 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2521 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2581 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2641 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2701 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2761 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2821 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2881 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2941 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3001 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3061 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3121 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3181 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3241 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3301 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3361 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3421 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3481 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3541 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3601 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3661 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3721 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3781 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3841 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3901 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3961 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4021 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4081 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4141 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4201 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4261 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4321 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4381 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4441 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4501 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4561 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4621 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4681 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     4741 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     4801 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     4861 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     4921 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     4981 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     5041 gggttttccc agtcacgacg ttgtaaaacg acggccagt
//
