LOCUS       V028242                 4490 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028242
VERSION     V028242
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             844..1575
                     /label="RTRAF(NM_016039)"
                     /note="RTRAF(NM_016039)"
                     /gene="RTRAF"
     CDS             1591..1614
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     polyA_signal    1629..1763
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1874..1890)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    1898..1914
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(1922..1952)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1967..1988
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2276..2864)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3035..3895)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(3896..4000)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     4474..4490
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcgcc
      841 accatgttcc gacgcaagtt gacggctctc gactaccaca accccgccgg cttcaactgc
      901 aaagatgaaa cagaatttag aaacttcatc gtttggcttg aagaccagaa aatcaggcac
      961 tacaagattg aagacagagg gaatttaaga aacatccaca gcagcgactg gcccaagttc
     1021 tttgaaaagt atctcagaga tgttaactgt cctttcaaga ttcaagatcg acaagaagct
     1081 attgactggc ttcttggttt agctgttaga cttgaatatg gagataatgc tgaaaaatac
     1141 aaggatttag tacctgataa ttcaaaaact gctgacaatg caactaaaaa tgcagaacca
     1201 ttgatcaatt tggatgtaaa taatcctgat tttaaggctg gtgtgatggc tttggctaac
     1261 ctgcttcaga ttcagcgtca tgatgattac ctggtaatgc ttaaggcaat tcggattttg
     1321 gttcaggagc gcctgacaca ggatgcagtt gctaaggcaa atcaaacaaa agagggctta
     1381 cctgttgctt tagacaaaca tattcttggt tttgacacag gagatgcagt tcttaatgaa
     1441 gctgctcaaa ttctgcgatt gctgcacata gaggagctca gagagctaca gacaaaaatc
     1501 aacgaagcca tagtagctgt tcaggcaatt attgctgatc caaagacaga ccacagactg
     1561 ggaaaagttg gaagaactcg aggtaccggc gactacaagg acgacgatga caagtaagcg
     1621 gccgcgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1681 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1741 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1801 agggggaggt gtgggaggtt ttttcggatc ctctagagtc gatctgcagg catgctagct
     1861 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     1921 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     1981 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2041 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2101 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2161 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2221 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2281 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2701 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2881 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2941 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3001 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3061 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3121 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3181 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3241 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3301 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3361 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3421 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3481 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3541 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3601 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3661 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3721 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3781 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3841 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3901 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3961 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4021 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     4081 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     4141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     4201 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     4261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4321 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     4381 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     4441 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
//
