LOCUS       pCMV-RIPK1(human)-HA    5783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047673
VERSION     V047673
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             832..858
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             889..2901
                     /label="RIPK1(NM_003804)"
                     /note="RIPK1(NM_003804)"
                     /gene="RIPK1"
     polyA_signal    2922..3056
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3167..3183)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3191..3207
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3215..3245)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3260..3281
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3569..4157)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4328..5188)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5189..5293)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5767..5783
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaccat gtacccatac
      841 gatgttccag attacgctct tatggccatg gaggcccgaa ttgccaccat gcaaccagac
      901 atgtccttga atgtcattaa gatgaaatcc agtgacttcc tggagagtgc agaactggac
      961 agcggaggct ttgggaaggt gtctctgtgt ttccacagaa cccagggact catgatcatg
     1021 aaaacagtgt acaaggggcc caactgcatt gagcacaacg aggccctctt ggaggaggcg
     1081 aagatgatga acagactgag acacagccgg gtggtgaagc tcctgggcgt catcatagag
     1141 gaagggaagt actccctggt gatggagtac atggagaagg gcaacctgat gcacgtgctg
     1201 aaagccgaga tgagtactcc gctttctgta aaaggaagga taattttgga aatcattgaa
     1261 ggaatgtgct acttacatgg aaaaggcgtg atacacaagg acctgaagcc tgaaaatatc
     1321 cttgttgata atgacttcca cattaagatc gcagacctcg gccttgcctc ctttaagatg
     1381 tggagcaaac tgaataatga agagcacaat gagctgaggg aagtggacgg caccgctaag
     1441 aagaatggcg gcaccctcta ctacatggcg cccgagcacc tgaatgacgt caacgcaaag
     1501 cccacagaga agtcggatgt gtacagcttt gctgtagtac tctgggcgat atttgcaaat
     1561 aaggagccat atgaaaatgc tatctgtgag cagcagttga taatgtgcat aaaatctggg
     1621 aacaggccag atgtggatga catcactgag tactgcccaa gagaaattat cagtctcatg
     1681 aagctctgct gggaagcgaa tccggaagct cggccgacat ttcctggcat tgaagaaaaa
     1741 tttaggcctt tttatttaag tcaattagaa gaaagtgtag aagaggacgt gaagagttta
     1801 aagaaagagt attcaaacga aaatgcagtt gtgaagagaa tgcagtctct tcaacttgat
     1861 tgtgtggcag taccttcaag ccggtcaaat tcagccacag aacagcctgg ttcactgcac
     1921 agttcccagg gacttgggat gggtcctgtg gaggagtcct ggtttgctcc ttccctggag
     1981 cacccacaag aagagaatga gcccagcctg cagagtaaac tccaagacga agccaactac
     2041 catctttatg gcagccgcat ggacaggcag acgaaacagc agcccagaca gaatgtggct
     2101 tacaacagag aggaggaaag gagacgcagg gtctcccatg acccttttgc acagcaaaga
     2161 ccttacgaga attttcagaa tacagaggga aaaggcactg cttattccag tgcagccagt
     2221 catggtaatg cagtgcacca gccctcaggg ctcaccagcc aacctcaagt actgtatcag
     2281 aacaatggat tatatagctc acatggcttt ggaacaagac cactggatcc aggaacagca
     2341 ggtcccagag tttggtacag gccaattcca agtcatatgc ctagtctgca taatatccca
     2401 gtgcctgaga ccaactatct aggaaataca cccaccatgc cattcagctc cttgccacca
     2461 acagatgaat ctataaaata taccatatac aatagtactg gcattcagat tggagcctac
     2521 aattatatgg agattggtgg gacgagttca tcactactag acagcacaaa tacgaacttc
     2581 aaagaagagc cagctgctaa gtaccaagct atctttgata ataccactag tctgacggat
     2641 aaacacctgg acccaatcag ggaaaatctg ggaaagcact ggaaaaactg tgcccgtaaa
     2701 ctgggcttca cacagtctca gattgatgaa attgaccatg actatgagcg agatggactg
     2761 aaagaaaagg tttaccagat gctccaaaag tgggtgatga gggaaggcat aaagggagcc
     2821 acggtgggga agctggccca ggcgctccac cagtgttcca ggatcgacct tctgagcagc
     2881 ttgatttacg tcagccagaa ctaaggtacc gcggccgcgg ggatccagac atgataagat
     2941 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     3001 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     3061 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttcgg
     3121 atcctctaga gtcgatctgc aggcatgcta gcttggcgta atcatggtca tagctgtttc
     3181 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3241 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     3301 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     3361 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3421 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     4261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4321 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     4981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     5041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     5101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     5161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     5221 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     5281 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     5341 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     5401 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     5461 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     5521 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     5581 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     5641 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     5701 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     5761 gacgttgtaa aacgacggcc agt
//
