LOCUS V029363 6889 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V029363 VERSION V029363 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..2580 /label="SOAT1(NM_003101)" /note="SOAT1(NM_003101)" /gene="SOAT1" CDS 2587..2604 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" polyA_signal 2651..2875 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2921..3349 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3363..3692 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3759..4358 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 4532..4653 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4702..4718) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4726..4742 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4750..4780) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4795..4816 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5104..5689) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5860..6720) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6721..6825) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atggtgggtg aagagaagat gtctctaaga 961 aaccggctgt caaagtccag ggaaaatcct gaggaagatg aagaccagag aaaccctgca 1021 aaggagtccc tagagacacc tagtaatggt cgaattgaca taaaacagtt gatagcaaag 1081 aagataaagt tgacagcaga ggcagaggaa ttgaagccat tttttatgaa ggaagttggc 1141 agtcactttg atgattttgt gaccaatctc attgaaaagt cagcatcatt agataatggt 1201 gggtgcgctc tcacaacctt ttctgttctt gaaggagaga aaaacaacca tagagcgaag 1261 gatttgagag cacctccaga acaaggaaag atttttattg caaggcgctc tctcttagat 1321 gaactgcttg aagtggacca catcagaaca atatatcaca tgtttattgc cctcctcatt 1381 ctctttatcc tcagcacact tgtagtagat tacattgatg aaggaaggct ggtgcttgag 1441 ttcagcctcc tgtcttatgc ttttggcaaa tttcctaccg ttgtttggac ctggtggatc 1501 atgttcctgt ctacattttc agttccctat tttctgtttc aacattgggc cactggctat 1561 agcaagagtt ctcatccgct gatccgttct ctcttccatg gctttctttt catgatcttc 1621 cagattggag ttctaggttt tggaccaaca tatgttgtgt tagcatatac actgccacca 1681 gcttcccggt tcatcattat attcgagcag attcgttttg taatgaaggc ccactcattt 1741 gtcagagaga acgtgcctcg ggtactaaat tcagctaagg agaaatcaag cactgttcca 1801 atacctacag tcaaccagta tttgtacttc ttatttgctc ctacccttat ctaccgtgac 1861 agctatccca ggaatcccac tgtaagatgg ggttatgtcg ctatgaagtt tgcacaggtc 1921 tttggttgct ttttctatgt gtactacatc tttgaaaggc tttgtgcccc cttgtttcgg 1981 aatatcaaac aggagccctt cagcgctcgt gttctggtcc tatgtgtatt taactccatc 2041 ttgccaggtg tgctgattct cttccttact ttttttgcct ttttgcactg ctggctcaat 2101 gcctttgctg agatgttacg ctttggtgac aggatgttct ataaggattg gtggaactcc 2161 acgtcatact ccaactatta tagaacctgg aatgtggtgg tccatgactg gctatattac 2221 tatgcttaca aggactttct ctggtttttc tccaagagat tcaaatctgc tgccatgtta 2281 gctgtctttg ctgtatctgc tgtagtacac gaatatgcct tggctgtttg cttgagcttt 2341 ttctatcccg tgctcttcgt gctcttcatg ttctttggaa tggctttcaa cttcattgtc 2401 aatgatagtc ggaaaaagcc gatttggaat gttctgatgt ggacttctct tttcttgggc 2461 aatggagtct tactctgctt ttattctcaa gaatggtatg cacgtcagca ctgtcctctg 2521 aaaaatccca catttttgga ttatgtccgg ccacgttcct ggacttgtcg ttacgtgttt 2581 gatatccatc atcaccatca ccattgactc gagtctagag ggcccgttta aacccgctga 2641 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct 2701 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca 2761 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag 2821 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct 2881 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca 2941 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 3001 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 3061 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 3121 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt 3181 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga 3241 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 3301 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 3361 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 3421 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 3481 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 3541 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 3601 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 3661 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 3721 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gaccgagtac aagcccacgg 3781 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg 3841 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg 3901 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 3961 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 4021 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 4081 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 4141 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 4201 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 4261 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 4321 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc gggactctgg ggttcgaaat 4381 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta 4441 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg 4501 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta 4561 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag 4621 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag 4681 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 4741 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 4801 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 4861 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 4921 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 4981 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 5041 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 5101 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 5161 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 5221 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 5281 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 5341 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 5401 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 5461 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 5521 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 5581 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 5641 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 5701 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 5761 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 5821 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 5881 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 5941 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6001 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6061 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 6121 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 6181 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 6241 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 6301 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 6361 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 6421 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 6481 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 6541 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 6601 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 6661 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 6721 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 6781 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 6841 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt //