LOCUS       V047501                 8441 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047501
VERSION     V047501
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4063
                     /label="SMARCA5(NM_003601)"
                     /note="SMARCA5(NM_003601)"
                     /gene="SMARCA5"
     CDS             4070..4096
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4100..4126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4130..4156
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4203..4427
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4473..4901
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4915..5244
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5311..5910
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    6084..6205
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6254..6270)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6278..6294
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6302..6332)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6347..6368
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6656..7241)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7412..8272)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8273..8377)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tcgtccgcgg ccgagcctcc gccacccccg cctcccgaga gcgcgccttc
      961 caagcccgca gcctcgatcg ccagcggcgg gagcaacagc agcaacaaag gcggccccga
     1021 aggcgtcgcg gcgcaggcgg ttgcgtctgc ggccagcgct ggtcccgcag acgccgagat
     1081 ggaggaaata tttgatgatg cgtcacctgg aaagcaaaag gaaatccaag aaccagatcc
     1141 tacctatgaa gaaaaaatgc aaactgaccg ggcaaataga ttcgagtatt tattaaagca
     1201 gacagaactt tttgcacatt tcattcaacc tgctgctcag aagactccaa cttcaccttt
     1261 gaagatgaaa ccagggcgcc cacgaataaa aaaagatgag aagcagaact tactatccgt
     1321 tggcgattac cgacaccgta gaacagagca agaggaggat gaagagctat taacagaaag
     1381 ctccaaagca accaatgttt gcactcgatt tgaagactct ccatcgtatg taaaatgggg
     1441 taaactgaga gattatcagg tccgaggatt aaactggctc atttctttgt atgagaatgg
     1501 catcaatggt atccttgcag atgaaatggg cctaggaaag actcttcaaa caatttctct
     1561 tcttgggtac atgaaacatt atagaaacat tcctgggcct catatggttt tggttcctaa
     1621 gtctacatta cacaactgga tgagtgaatt caagagatgg gtaccaacac ttagatctgt
     1681 ttgtttgata ggagataaag aacaaagagc tgcttttgtc agagacgttt tattaccggg
     1741 agaatgggat gtatgtgtaa catcttatga aatgcttatt aaagagaagt ctgtgttcaa
     1801 aaaatttaat tggagatact tagtaataga tgaagctcac aggatcaaaa atgaaaaatc
     1861 taagttgtca gaaatagtga gggaattcaa gactacaaat agactattat taactggaac
     1921 acctcttcag aacaacttgc atgagctgtg gtcacttctt aactttctgt tgccagatgt
     1981 gtttaattca gcagatgact ttgattcctg gtttgataca aacaactgcc ttggggatca
     2041 aaaactagtt gagaggcttc atatggtttt gcgtccattc ctccttcgtc gaattaaggc
     2101 tgatgttgaa aagagtttgc ctccaaagaa ggaagtaaaa atctatgtgg gcctcagcaa
     2161 aatgcaaagg gaatggtata ctcggatatt aatgaaggat atagatatac tcaactcagc
     2221 aggcaagatg gacaaaatga ggttattgaa catcctaatg cagttgagaa aatgttgtaa
     2281 tcatccatat ctctttgatg gagcagaacc tggtccacct tatacaacag atatgcatct
     2341 agtaaccaac agtggcaaaa tggtggtttt agacaagctg ctccctaagt taaaagaaca
     2401 aggttcacga gtactaatct tcagtcaaat gacaagggta ttggacattt tggaagatta
     2461 ttgcatgtgg agaaattatg agtactgcag gttggatggt cagacacccc atgatgagag
     2521 acaagactcc atcaatgcat acaatgaacc aaacagcaca aagtttgttt tcatgttaag
     2581 cacgcgtgct ggtggtcttg gcatcaatct tgcgactgct gatgtagtaa ttttgtatga
     2641 ttctgattgg aatccccaag tagatcttca ggctatggac cgagcacata gaattgggca
     2701 gactaagaca gtcagagtgt tccgctttat aactgataac actgtagaag aaagaatagt
     2761 agaacgtgct gagatgaaac tcagactgga ttcaatagtc attcaacaag ggaggcttgt
     2821 ggatcagaat ctgaacaaaa ttgggaaaga tgaaatgctt caaatgatta gacatggagc
     2881 aacacatgtg tttgcttcaa aggaaagtga gatcactgat gaagatatcg atggtatttt
     2941 ggaaagaggt gcaaagaaga ctgcagagat gaatgaaaag ctctccaaga tgggcgaaag
     3001 ttcacttaga aactttacaa tggatacaga gtcaagtgtt tataacttcg aaggagaaga
     3061 ctatagagaa aaacaaaaga ttgcattcac agagtggatt gaaccaccta aacgagaaag
     3121 aaaagccaac tatgccgttg atgcatattt cagggaagct cttcgtgtta gtgaacctaa
     3181 agcacccaag gctcctcgac ctccaaaaca acccaatgtt caggatttcc agttctttcc
     3241 tccacgttta tttgaattac tggaaaaaga aattctgttt tacagaaaaa ctattgggta
     3301 caaggtacct cgaaatcctg agctgcctaa tgcagcacag gcacaaaaag aagaacagct
     3361 taaaattgat gaagctgaat cccttaatga tgaagagtta gaggaaaaag agaagcttct
     3421 aacacaggga tttaccaatt ggaataagag agattttaac cagtttatca aagctaatga
     3481 gaagtggggt cgtgatgata ttgaaaatat agcaagagaa gtagaaggca aaactccaga
     3541 agaagtcatt gaatattcag ctgtgttttg ggaaaggtgc aacgagctcc aggacataga
     3601 gaagattatg gctcagattg aaaggggaga ggcgagaatt caaagaagaa taagcatcaa
     3661 gaaagcactt gacacaaaga ttggacggta caaagcacct tttcatcagc tgagaatatc
     3721 atatggtact aacaaaggaa aaaactatac tgaagaagaa gatcgttttc tgatttgtat
     3781 gcttcacaaa cttggatttg acaaagaaaa tgtttatgat gaattgcgac agtgtattcg
     3841 caactctcct cagttcagat ttgactggtt tcttaagtcc agaactgcaa tggagctcca
     3901 gaggagatgt aataccttaa ttactttgat tgaaagagaa aacatggaac tagaagaaaa
     3961 ggagaaggca gagaaaaaga aacgaggacc aaagccttca acacagaaac gtaaaatgga
     4021 tggcgcacct gatggtcgag gaagaaaaaa gaagctgaaa ctactcgagt acccatacga
     4081 cgtacctgat tatgcaactt atccttatga tgtacctgat tatgctagct atccttatga
     4141 tgtcccagac tacgcatagc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     4201 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     4261 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     4321 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4381 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     4441 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     4501 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4561 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4621 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4681 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4741 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4801 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4861 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     4921 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4981 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5041 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     5101 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5161 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     5221 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     5281 gatcaagaga caggatgagg atcgtttcgc atgaccgagt acaagcccac ggtgcgcctc
     5341 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5401 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     5461 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     5521 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     5581 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     5641 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     5701 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     5761 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     5821 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     5881 tggtgcatga cccgcaagcc cggtgcctga gcgggactct ggggttcgaa atgaccgacc
     5941 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6001 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6061 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6121 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6181 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6241 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6301 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6361 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6421 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6481 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6541 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6601 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6661 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6721 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6781 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6841 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6901 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6961 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7021 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7081 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7141 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     7201 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7261 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7321 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7381 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7441 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7501 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7561 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7621 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7681 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     7741 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7801 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7861 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7921 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7981 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8041 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8101 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8161 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8221 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8281 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8341 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8401 ctgacgtcga cggatcggga gatctcccga tcccctatgg t
//
