LOCUS V030779 8321 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V030779 VERSION V030779 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..3949 /label="PARP1(NM_001618)" /note="PARP1(NM_001618)" /gene="PARP1" CDS 3956..3982 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 3986..4012 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 4016..4042 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 4083..4307 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4353..4781 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4795..5124 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5191..5790 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 5964..6085 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6134..6150) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6158..6174 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6182..6212) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6227..6248 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6536..7121) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7292..8152) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8153..8257) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gcggagtctt cggataagct ctatcgagtc gagtacgcca agagcgggcg 961 cgcctcttgc aagaaatgca gcgagagcat ccccaaggac tcgctccgga tggccatcat 1021 ggtgcagtcg cccatgtttg atggaaaagt cccacactgg taccacttct cctgcttctg 1081 gaaggtgggc cactccatcc ggcaccctga cgttgaggtg gatgggttct ctgagcttcg 1141 gtgggatgac cagcagaaag tcaagaagac agcggaagct ggaggagtga caggcaaagg 1201 ccaggatgga attggtagca aggcagagaa gactctgggt gactttgcag cagagtatgc 1261 caagtccaac agaagtacgt gcaaggggtg tatggagaag atagaaaagg gccaggtgcg 1321 cctgtccaag aagatggtgg acccggagaa gccacagcta ggcatgattg accgctggta 1381 ccatccaggc tgctttgtca agaacaggga ggagctgggt ttccggcccg agtacagtgc 1441 gagtcagctc aagggcttca gcctccttgc tacagaggat aaagaagccc tgaagaagca 1501 gctcccagga gtcaagagtg aaggaaagag aaaaggcgat gaggtggatg gagtggatga 1561 agtggcgaag aagaaatcta aaaaagaaaa agacaaggat agtaagcttg aaaaagccct 1621 aaaggctcag aacgacctga tctggaacat caaggacgag ctaaagaaag tgtgttcaac 1681 taatgacctg aaggagctac tcatcttcaa caagcagcaa gtgccttctg gggagtcggc 1741 gatcttggac cgagtagctg atggcatggt gttcggtgcc ctccttccct gcgaggaatg 1801 ctcgggtcag ctggtcttca agagcgatgc ctattactgc actggggacg tcactgcctg 1861 gaccaagtgt atggtcaaga cacagacacc caaccggaag gagtgggtaa ccccaaagga 1921 attccgagaa atctcttacc tcaagaaatt gaaggttaaa aaacaggacc gtatattccc 1981 cccagaaacc agcgcctccg tggcggccac gcctccgccc tccacagcct cggctcctgc 2041 tgctgtgaac tcctctgctt cagcagataa gccattatcc aacatgaaga tcctgactct 2101 cgggaagctg tcccggaaca aggatgaagt gaaggccatg attgagaaac tcggggggaa 2161 gttgacgggg acggccaaca aggcttccct gtgcatcagc accaaaaagg aggtggaaaa 2221 gatgaataag aagatggagg aagtaaagga agccaacatc cgagttgtgt ctgaggactt 2281 cctccaggac gtctccgcct ccaccaagag ccttcaggag ttgttcttag cgcacatctt 2341 gtccccttgg ggggcagagg tgaaggcaga gcctgttgaa gttgtggccc caagagggaa 2401 gtcaggggct gcgctctcca aaaaaagcaa gggccaggtc aaggaggaag gtatcaacaa 2461 atctgaaaag agaatgaaat taactcttaa aggaggagca gctgtggatc ctgattctgg 2521 actggaacac tctgcgcatg tcctggagaa aggtgggaag gtcttcagtg ccacccttgg 2581 cctggtggac atcgttaaag gaaccaactc ctactacaag ctgcagcttc tggaggacga 2641 caaggaaaac aggtattgga tattcaggtc ctggggccgt gtgggtacgg tgatcggtag 2701 caacaaactg gaacagatgc cgtccaagga ggatgccatt gagcacttca tgaaattata 2761 tgaagaaaaa accgggaacg cttggcactc caaaaatttc acgaagtatc ccaaaaagtt 2821 ctaccccctg gagattgact atggccagga tgaagaggca gtgaagaagc tgacagtaaa 2881 tcctggcacc aagtccaagc tccccaagcc agttcaggac ctcatcaaga tgatctttga 2941 tgtggaaagt atgaagaaag ccatggtgga gtatgagatc gaccttcaga agatgccctt 3001 ggggaagctg agcaaaaggc agatccaggc cgcatactcc atcctcagtg aggtccagca 3061 ggcggtgtct cagggcagca gcgactctca gatcctggat ctctcaaatc gcttttacac 3121 cctgatcccc cacgactttg ggatgaagaa gcctccgctc ctgaacaatg cagacagtgt 3181 gcaggccaag gtggaaatgc ttgacaacct gctggacatc gaggtggcct acagtctgct 3241 caggggaggg tctgatgata gcagcaagga tcccatcgat gtcaactatg agaagctcaa 3301 aactgacatt aaggtggttg acagagattc tgaagaagcc gagatcatca ggaagtatgt 3361 taagaacact catgcaacca cacacaatgc gtatgacttg gaagtcatcg atatctttaa 3421 gatagagcgt gaaggcgaat gccagcgtta caagcccttt aagcagcttc ataaccgaag 3481 attgctgtgg cacgggtcca ggaccaccaa ctttgctggg atcctgtccc agggtcttcg 3541 gatagccccg cctgaagcgc ccgtgacagg ctacatgttt ggtaaaggga tctatttcgc 3601 tgacatggtc tccaagagtg ccaactactg ccatacgtct cagggagacc caataggctt 3661 aatcctgttg ggagaagttg cccttggaaa catgtatgaa ctgaagcacg cttcacatat 3721 cagcaagtta cccaagggca agcacagtgt caaaggtttg ggcaaaacta cccctgatcc 3781 ttcagctaac attagtctgg atggtgtaga cgttcctctt gggaccggga tttcatctgg 3841 tgtgaatgac acctctctac tatataacga gtacattgtc tatgatattg ctcaggtaaa 3901 tctgaagtat ctgctgaaac tgaaattcaa ttttaagacc tccctgtggc tcgagtaccc 3961 atacgacgta cctgattatg caacttatcc ttatgatgta cctgattatg ctagctatcc 4021 ttatgatgtc ccagactacg catagtctag agggcccgtt taaacccgct gatcagcctc 4081 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 4141 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 4201 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 4261 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga 4321 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc 4381 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 4441 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 4501 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 4561 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 4621 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 4681 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 4741 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt 4801 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 4861 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 4921 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 4981 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 5041 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt 5101 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct 5161 gatcaagaga caggatgagg atcgtttcgc atgaccgagt acaagcccac ggtgcgcctc 5221 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac 5281 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa 5341 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 5401 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 5461 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 5521 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 5581 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 5641 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 5701 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 5761 tggtgcatga cccgcaagcc cggtgcctga gcgggactct ggggttcgaa atgaccgacc 5821 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 5881 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 5941 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 6001 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 6061 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 6121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 6181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 6241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 6301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 6361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 6421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 6481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 6541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 6601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 6661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 6721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 6781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6961 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 7021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg 7081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 7141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 7201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 7261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 7321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 7381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 7441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 7501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 7561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 7621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 7681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 7741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 7801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 7861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 7921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 7981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 8041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 8101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 8161 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 8221 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 8281 ctgacgtcga cggatcggga gatctcccga tcccctatgg t //