LOCUS V043089 6115 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043089 VERSION V043089 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..1743 /label="OTUB1(NM_017670)" /note="OTUB1(NM_017670)" /gene="OTUB1" CDS 1750..1776 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1780..1806 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" CDS 1810..1836 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 1877..2101 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2147..2575 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2589..2918 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2985..3584 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 3758..3879 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3928..3944) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3952..3968 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3976..4006) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4021..4042 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4330..4915) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5086..5946) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5947..6051) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atggcggcgg aggaacctca gcagcagaag 961 caggagccgc tgggcagcga ctccgaaggt gttaactgtc tggcctatga tgaagccatc 1021 atggctcagc aggaccgaat tcagcaagag attgctgtgc agaaccctct ggtgtcagag 1081 cggctggagc tctcggtcct atacaaggag tatgctgaag atgacaacat ctatcaacag 1141 aagatcaagg acctccacaa aaagtactcg tacatccgca agaccaggcc tgacggcaac 1201 tgtttctatc gggctttcgg attctcccac ttggaggcac tgctggatga cagcaaggag 1261 ttgcagcggt tcaaggctgt gtctgccaag agcaaggaag acctggtgtc ccagggcttc 1321 actgaattca caattgagga tttccacaac acgttcatgg acctgattga gcaggtggag 1381 aagcagacct ctgtcgccga cctgctggcc tccttcaatg accagagcac ctccgactac 1441 cttgtggtct acctgcggct gctcacctcg ggctacctgc agcgcgagag caagttcttc 1501 gagcacttca tcgagggtgg acggactgtc aaggagttct gccagcagga ggtggagccc 1561 atgtgcaagg agagcgacca catccacatc attgcgctgg cccaggccct cagcgtgtcc 1621 atccaggtgg agtacatgga ccgcggcgag ggcggcacca ccaatccgca catcttccct 1681 gagggctccg agcccaaggt ctaccttctc taccggcctg gacactacga tatcctctac 1741 aaactcgagt acccatacga cgtacctgat tatgcaactt atccttatga tgtacctgat 1801 tatgctagct atccttatga tgtcccagac tacgcatagt ctagagggcc cgtttaaacc 1861 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc 1921 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa 1981 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac 2041 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg 2101 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc 2161 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 2221 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 2281 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 2341 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 2401 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 2461 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 2521 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc 2581 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 2641 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 2701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 2761 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 2821 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt 2881 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat 2941 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgacc gagtacaagc 3001 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg 3061 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg 3121 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 3181 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 3241 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 3301 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 3361 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 3421 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 3481 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg 3541 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagcggga ctctggggtt 3601 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 3661 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 3721 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 3781 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 3841 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 3901 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 3961 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 4021 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 4081 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 4141 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 4201 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 4261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4321 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 4801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4861 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 4921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 4981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 5041 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 5101 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 5161 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 5221 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 5281 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 5341 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 5401 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 5461 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 5521 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 5581 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 5641 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 5701 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 5761 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 5821 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 5881 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 5941 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 6001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 6061 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggt //