LOCUS V046438 8195 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046438 VERSION V046438 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..3814 /label="NFKB1(NM_003998)" /note="NFKB1(NM_003998)" /gene="NFKB1" CDS 3821..3910 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 3957..4181 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 4227..4655 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4669..4998 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5065..5664 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 5838..5959 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(6008..6024) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 6032..6048 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(6056..6086) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 6101..6122 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6410..6995) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(7166..8026) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(8027..8131) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gcagaagatg atccatattt gggaaggcct gaacaaatgt ttcatttgga 961 tccttctttg actcatacaa tatttaatcc agaagtattt caaccacaga tggcactgcc 1021 aacagcagat ggcccatacc ttcaaatatt agagcaacct aaacagagag gatttcgttt 1081 ccgttatgta tgtgaaggcc catcccatgg tggactacct ggtgcctcta gtgaaaagaa 1141 caagaagtct taccctcagg tcaaaatctg caactatgtg ggaccagcaa aggttattgt 1201 tcagttggtc acaaatggaa aaaatatcca cctgcatgcc cacagcctgg tgggaaaaca 1261 ctgtgaggat gggatctgca ctgtaactgc tggacccaag gacatggtgg tcggcttcgc 1321 aaacctgggt atacttcatg tgacaaagaa aaaagtattt gaaacactgg aagcacgaat 1381 gacagaggcg tgtataaggg gctataatcc tggactcttg gtgcaccctg accttgccta 1441 tttgcaagca gaaggtggag gggaccggca gctgggagat cgggaaaaag agctaatccg 1501 ccaagcagct ctgcagcaga ccaaggagat ggacctcagc gtggtgcggc tcatgtttac 1561 agcttttctt ccggatagca ctggcagctt cacaaggcgc ctggaacccg tggtatcaga 1621 cgccatctat gacagtaaag cccccaatgc atccaacttg aaaattgtaa gaatggacag 1681 gacagctgga tgtgtgactg gaggggagga aatttatctt ctttgtgaca aagttcagaa 1741 agatgacatc cagattcgat tttatgaaga ggaagaaaat ggtggagtct gggaaggatt 1801 tggagatttt tcccccacag atgttcatag acaatttgcc attgtcttca aaactccaaa 1861 gtataaagat attaatatta caaaaccagc ctctgtgttt gtccagcttc ggaggaaatc 1921 tgacttggaa actagtgaac caaaaccttt cctctactat cctgaaatca aagataaaga 1981 agaagtgcag aggaaacgtc agaagctcat gcccaatttt tcggatagtt tcggcggtgg 2041 tagtggtgct ggagctggag gcggaggcat gtttggtagt ggcggtggag gagggggcac 2101 tggaagtaca ggtccagggt atagcttccc acactatgga tttcctactt atggtgggat 2161 tactttccat cctggaacta ctaaatctaa tgctgggatg aagcatggaa ccatggacac 2221 tgaatctaaa aaggaccctg aaggttgtga caaaagtgat gacaaaaaca ctgtaaacct 2281 ctttgggaaa gttattgaaa ccacagagca agatcaggag cccagcgagg ccaccgttgg 2341 gaatggtgag gtcactctaa cgtatgcaac aggaacaaaa gaagagagtg ctggagttca 2401 ggataacctc tttctagaga aggctatgca gcttgcaaag aggcatgcca atgccctttt 2461 cgactacgcg gtgacaggag acgtgaagat gctgctggcc gtccagcgcc atctcactgc 2521 tgtgcaggat gagaatgggg acagtgtctt acacttagca atcatccacc ttcattctca 2581 acttgtgagg gatctactag aagtcacatc tggtttgatt tctgatgaca ttatcaacat 2641 gagaaatgat ctgtaccaga cgcccttgca cttggcagtg atcactaagc aggaagatgt 2701 ggtggaggat ttgctgaggg ctggggccga cctgagcctt ctggaccgct tgggtaactc 2761 tgttttgcac ctagctgcca aagaaggaca tgataaagtt ctcagtatct tactcaagca 2821 caaaaaggca gcactacttc ttgaccaccc caacggggac ggtctgaatg ccattcatct 2881 agccatgatg agcaatagcc tgccatgttt gctgctgctg gtggccgctg gggctgacgt 2941 caatgctcag gagcagaagt ccgggcgcac agcactgcac ctggctgtgg agcacgacaa 3001 catctcattg gcaggctgcc tgctcctgga gggtgatgcc catgtggaca gtactaccta 3061 cgatggaacc acacccctgc atatagcagc tgggagaggg tccaccaggc tggcagctct 3121 tctcaaagca gcaggagcag atcccctggt ggagaacttt gagcctctct atgacctgga 3181 tgactcttgg gaaaatgcag gagaggatga aggagttgtg cctggaacca cgcctctaga 3241 tatggccacc agctggcagg tatttgacat attaaatggg aaaccatatg agccagagtt 3301 tacatctgat gatttactag cacaaggaga catgaaacag ctggctgaag atgtgaagct 3361 gcagctgtat aagttactag aaattcctga tccagacaaa aactgggcta ctctggcgca 3421 gaaattaggt ctggggatac ttaataatgc cttccggctg agtcctgctc cttccaaaac 3481 acttatggac aactatgagg tctctggggg tacagtcaga gagctggtgg aggccctgag 3541 acaaatgggc tacaccgaag caattgaagt gatccaggca gcctccagcc cagtgaagac 3601 cacctctcag gcccactcgc tgcctctctc gcctgcctcc acaaggcagc aaatagacga 3661 gctccgagac agtgacagtg tctgcgacag cggcgtggag acatccttcc gcaaactcag 3721 ctttaccgag tctctgacca gtggtgcctc actgctaact ctcaacaaaa tgccccatga 3781 ttatgggcag gaaggacctc tagaaggcaa aattgatatc gaacaaaaac taatatcaga 3841 ggaagatttg gaacagaaac taatcagtga agaagattta gagcaaaagt taatttccga 3901 ggaggactta tagctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt 3961 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 4021 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 4081 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 4141 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 4201 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 4261 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 4321 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 4381 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 4441 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 4501 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 4561 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 4621 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 4681 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4741 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4801 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4861 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4921 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4981 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa 5041 gagacaggat gaggatcgtt tcgcatgacc gagtacaagc ccacggtgcg cctcgccacc 5101 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc 5161 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc 5221 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg 5281 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc 5341 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc 5401 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc 5461 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag 5521 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag 5581 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc 5641 atgacccgca agcccggtgc ctgagcggga ctctggggtt cgaaatgacc gaccaagcga 5701 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct 5761 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg 5821 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata 5881 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 5941 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt 6001 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 6061 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 6121 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 6181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 6241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 6301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 6361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 6421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 6481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 6541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 6601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 6661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 6721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 6781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 6841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 6901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca 6961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 7021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 7081 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 7141 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 7201 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 7261 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 7321 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 7381 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 7441 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 7501 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 7561 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 7621 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 7681 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 7741 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 7801 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7861 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7921 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7981 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 8041 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 8101 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 8161 tcgacggatc gggagatctc ccgatcccct atggt //