LOCUS V028325 7325 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V028325 VERSION V028325 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..3007 /label="MYBL2(NM_002466)" /note="MYBL2(NM_002466)" /gene="MYBL2" polyA_signal 3087..3311 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3357..3785 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3799..4128 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4195..4794 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 4968..5089 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5138..5154) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5162..5178 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5186..5216) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5231..5252 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5540..6125) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6296..7156) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7157..7261) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg tctcggcgga cgcgctgcga ggatctggat gagctgcact accaggacac 961 agattcagat gtgccggagc agagggatag caagtgcaag gtcaaatgga cccatgagga 1021 ggacgagcag ctgagggccc tggtgaggca gtttggacag caggactgga agttcctggc 1081 cagccacttc cctaaccgca ctgaccagca atgccagtac aggtggctga gagttttgaa 1141 tccagacctt gtcaaggggc catggaccaa agaggaagac caaaaagtca tcgagctggt 1201 taagaagtat ggcacaaagc agtggacact gattgccaag cacctgaagg gccggctggg 1261 gaagcagtgc cgtgaacgct ggcacaacca cctcaaccct gaggtgaaga agtcttgctg 1321 gaccgaggag gaggaccgca tcatctgcga ggcccacaag gtgctgggca accgctgggc 1381 cgagatcgcc aagatgttgc cagggaggac agacaatgct gtgaagaatc actggaactc 1441 taccatcaaa aggaaggtgg acacaggagg cttcttgagc gagtccaaag actgcaagcc 1501 cccagtgtac ttgctgctgg agctcgagga caaggacggc ctccagagtg cccagcccac 1561 ggaaggccag ggaagtcttc tgaccaactg gccctccgtc cctcctacca taaaggagga 1621 ggaaaacagt gaggaggaac ttgcagcagc caccacatcg aaggaacagg agcccatcgg 1681 tacagatctg gacgcagtgc gaacaccaga gcccttggag gaattcccga agcgtgagga 1741 ccaggaaggc tccccaccag aaacgagcct gccttacaag tgggtggtgg aggcagctaa 1801 cctcctcatc cctgctgtgg gttctagcct ctctgaagcc ctggacttga tcgagtcgga 1861 ccctgatgct tggtgtgacc tgagtaaatt tgacctccct gaggaaccat ctgcagagga 1921 cagtatcaac aacagcctag tgcagctgca agcgtcacat cagcagcaag tcctgccacc 1981 ccgccagcct tccgccctgg tgcccagtgt gaccgagtac cgcctggatg gccacaccat 2041 ctcagacctg agccggagca gccggggcga gctgatcccc atctccccca gcactgaagt 2101 cgggggctct ggcattggca caccgccctc tgtgctcaag cggcagagga agaggcgtgt 2161 ggctctgtcc cctgtcactg agaatagcac cagtctgtcc ttcctggatt cctgtaacag 2221 cctcacgccc aagagcacac ctgttaagac cctgcccttc tcgccctccc agtttctgaa 2281 cttctggaac aaacaggaca cattggagct ggagagcccc tcgctgacat ccaccccagt 2341 gtgcagccag aaggtggtgg tcaccacacc actgcaccgg gacaagacac ccctgcacca 2401 gaaacatgct gcgtttgtaa ccccagatca gaagtactcc atggacaaca ctccccacac 2461 gccaaccccg ttcaagaacg ccctggagaa gtacggaccc ctgaagcccc tgccacagac 2521 cccgcacctg gaggaggact tgaaggaggt gctgcgttct gaggctggca tcgaactcat 2581 catcgaggac gacatcaggc ccgagaagca gaagaggaag cctgggctgc ggcggagccc 2641 catcaagaaa gtccggaagt ctctggctct tgacattgtg gatgaggatg tgaagctgat 2701 gatgtccaca ctgcccaagt ctctatcctt gccgacaact gccccttcaa actcttccag 2761 cctcaccctg tcaggtatca aagaagacaa cagcttgctc aaccagggct tcttgcaggc 2821 caagcccgag aaggcagcag tggcccagaa gccccgaagc cacttcacga cacctgcccc 2881 tatgtccagt gcctggaaga cggtggcctg cggggggacc agggaccagc ttttcatgca 2941 ggagaaagcc cggcagctcc tgggccgcct gaagcccagc cacacatctc ggaccctcat 3001 cttgtcctga gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc 3061 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 3121 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 3181 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 3241 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 3301 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 3361 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3421 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3481 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3541 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 3601 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3661 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 3721 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3781 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 3841 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 3901 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 3961 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4021 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 4081 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 4141 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgacc 4201 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc 4261 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac 4321 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 4381 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 4441 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 4501 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 4561 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4621 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 4681 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 4741 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagcggga 4801 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt 4861 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga 4921 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg 4981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta 5101 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa 5161 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 5221 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 5281 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 5341 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 5401 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5461 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 5521 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 5581 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 5641 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 5701 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 5761 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 5821 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 5881 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 5941 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 6001 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6061 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 6121 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 6181 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 6241 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 6301 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 6361 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 6421 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 6481 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 6541 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6601 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6661 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6721 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6781 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6841 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6901 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6961 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 7021 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 7081 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 7141 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 7201 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 7261 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 7321 atggt //