LOCUS       V023773                 9338 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V023773
VERSION     V023773
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4903
                     /label="MYBBP1A(NM_001105538)"
                     /note="MYBBP1A(NM_001105538)"
                     /gene="MYBBP1A"
     CDS             4919..4960
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     CDS             4967..4990
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     CDS             5030..5053
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin®, an engineered
                     form of streptavidin"
                     /transl_table=1
                     /translation="WSHPQFEK"
                     /label="Strep-Tag II"
     polyA_signal    5100..5324
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5370..5798
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5812..6141
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6208..6807
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    6981..7102
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7151..7167)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7175..7191
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7199..7229)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7244..7265
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7553..8138)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8309..9169)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9170..9274)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gagagccggg atcccgccca gccgatgtcg cctggagaag cgacgcagag
      961 tggcgcccgg cctgccgacc gctatggcct attgaagcac agtcgcgagt tcttggactt
     1021 cttctgggac attgcgaagc ctgagcagga gacgcgactt gcggccacgg agaagctgct
     1081 ggagtatctg cgtggcaggc cgaaggggtc cgagatgaaa tatgccctga agcgtctaat
     1141 cacgggactc ggggtcgggc gagaaacagc ccggccctgc tacagtttgg ccctggcaca
     1201 gctgttacag tcttttgaag acctcccctt gtgcagcatc ctgcagcaga tacaagaaaa
     1261 atatgacctg catcaggtga agaaggcaat gctgagacct gctctctttg caaacctgtt
     1321 tggagtgctc gccctctttc agtcaggtcg gctggtgaag gaccaggagg cactgatgaa
     1381 gtcggtgaag ctgctgcagg ccctggccca gtaccaaaac cacttgcagg agcagccccg
     1441 gaaggccctg gtggacatcc tctccgaggt ctcgaaggcc acattgcagg agatcctgcc
     1501 ggaggtcctc aaagccgact tgaatataat actcagctcc cctgaacagc tagagctctt
     1561 cctcctggcc cagcagaagg tgccctccaa gctcaagaag ctggtgggat ccgtgaacct
     1621 attctcagat gagaatgtcc ccaggctggt gaatgtgctg aagatggccg cctcctctgt
     1681 gaagaaggac cgcaagctgc ccgccattgc tctggacctg ctccgcctgg cactcaagga
     1741 agacaagttc ccacggttct ggaaggaggt ggtggaacaa gggctgctga agatgcagtt
     1801 ctggccagcc agctacctgt gtttccgcct gctgggcgcg gccctgcccc tgctgaccaa
     1861 ggagcagctg cacctggtga tgcagggaga cgtgatccgc cattacgggg agcacgtgtg
     1921 cactgctaag ctcccaaagc agttcaagtt tgccccagag atggacgatt acgtgggcac
     1981 cttcctagag gggtgccagg atgaccctga gcggcagctg gccgtgctag tggccttctc
     2041 atctgtcacc aaccaaggcc tccctgtcac gcctactttc tggcgggtcg tgcggttcct
     2101 gagccctccg gccctgcagg gctatgtggc ctggctgcgg gccatgtttc tccagccaga
     2161 cctggactcc ttggttgact tcagcaccaa caaccagaag aaagcccagg attcatcgct
     2221 ccacatgcct gagcgagctg tgttccggct gaggaaatgg atcatctttc gattggtgag
     2281 cattgtggac agcctgcacc tggagatgga ggaggccttg actgagcagg tggccaggtt
     2341 ttgtttgttc cactcgttct ttgtcacaaa gaagcccaca tcccagatcc ctgagacaaa
     2401 gcacccgttc tccttccctt tggaaaacca ggcccgagag gctgtcagca gtgccttctt
     2461 cagtctgttg cagaccctca gcacgcagtt caagcaggca ccgggccaga cccagggtgg
     2521 gcagccctgg acctaccacc tggtgcagtt cgcagacctc ctgttgaatc acagccacaa
     2581 cgtgaccacc gtgacaccct tcactgcgca gcagcgccag gcctgggacc ggatgctgca
     2641 gactctgaag gagctggagg cccactccgc agaggccagg gctgctgcct tccagcacct
     2701 tctgctcctc gtgggcatcc acctcctcaa gtcccctgca gagagctgtg acctgctggg
     2761 tgacatccag acctgcatca ggaaaagtct gggagagaag ccccgccgga gccgcaccaa
     2821 gaccatcgac ccccaggaac ccccgtgggt agaggtgctg gtggagatct tgctggccct
     2881 gttggcccag cccagccacc tcatgcgcca ggtggcccgg agcgtgtttg gccacatctg
     2941 ctcccacctg accccgcgtg ccctgcagct aattctggat gtgctgaacc ccgagaccag
     3001 tgaggatgag aatgaccgtg tggtggtgac ggacgattct gatgagcggc ggctgaaggg
     3061 tgcagaggac aagagcgagg aaggtgagga caacagaagc tcagagagtg aagaggagag
     3121 cgagggggag gagagcgagg aggaggagcg cgacggggac gtggatcagg gcttccggga
     3181 acagctgatg accgtgctgc aggctgggaa ggcgctgggt ggagaggaca gtgagaacga
     3241 ggaggagctg ggggatgagg ccatgatggc cctggaccag agcctcgcca gcctctttgc
     3301 cgagcagaag ctgcgtatcc aggcccggcg agacgagaag aacaagctgc agaaggagaa
     3361 ggctctgcgg cgcgacttcc agatccgggt gctggacctg gtggaggtgc tagtgaccaa
     3421 gcagcccgag aatgccctgg tcctggagct gctggagccg ctgctgagca tcatccggcg
     3481 cagcctgcgc agcagcagct ccaaacagga gcaggacctt ctgcacaaga cggcgcgcat
     3541 cttcacgcac cacctgtgcc gtgcccggcg ctactgccac gacttgggtg agcgcgcagg
     3601 ggccctgcac gcccaggtgg agcggttggt gcagcaggct ggccgccagc ccgactcccc
     3661 caccgccctc taccacttca acgcctctct ctacctgctc cgggtcttga agggcaacac
     3721 tgctgagggc tgcgtgcatg agacacagga gaagcagaaa gctggcactg accccagcca
     3781 catgcccacg ggcccgcagg ctgccagctg cttggacttg aacctggtga cccgggtgta
     3841 ctcgacagca ctgagctcct tcctgaccaa gcgcaacagc cccctcacag ttcccatgtt
     3901 cctcagcctc ttctcccggc acccggtgct ctgtcagagc ctgctcccca tcctggtcca
     3961 gcatatcacg ggcccggtgc ggccccgtca tcaggcctgc ctgctgctcc agaagaccct
     4021 gtccatgcgg gaggtgaggt cgtgctttga ggaccccgag tggaagcagc tgatgggcca
     4081 ggtcctagca aaggtcaccg agaacttgcg cgtgctgggg gaggcgcaga ccaaggcgca
     4141 gcatcagcag gcactgtcct ccctggagct gctcaacgtt ctcttcagga cctgcaaaca
     4201 tgagaagctg accttggacc tgacggtgct cctgggtgtg ctgcaggggc aacagcagag
     4261 cctacagcag ggggcacact ccaccggctc cagccgcctg cacgacctct actggcaggc
     4321 catgaaaacc ctgggagtcc agcgccccaa gttggagaag aaggatgcca aggagatccc
     4381 cagtgccacc cagagcccca tcagtaagaa gcggaagaaa aagggattct tgccagagac
     4441 gaagaagcgc aagaaacgca agtcagagga tggcacgcca gcggaggatg gcacacctgc
     4501 agccaccggc gggagccagc cccccagcat gggcaggaag aagaggaaca ggacaaaggc
     4561 taaggtccca gcccaggcaa acgggacgcc aaccaccaag agtccagccc ctggcgcccc
     4621 cacccggagc cccagcaccc ctgccaaatc cccaaaactg cagaagaaaa accagaagcc
     4681 gtcccaggtg aatggagctc ccgggtcccc cacggaacct gcaggccaaa agcagcatca
     4741 gaaggctctt cccaaaaagg gggtcttggg caaatcacca ctgtccgcgc tggcacggaa
     4801 aaaggcaagg ctgtctttgg tcatcaggag tcccagcctg cttcagagtg gggccaagaa
     4861 gaaagcacag actctgagat tcacaatcag cagctctaaa aaagcggccg cgggtagcgg
     4921 taagcctatc cctaaccctc tcctcggtct cgattctacg ggtagctgga gccatccgca
     4981 gtttgaaaaa ggtggtggta gcggtggtgg ttcaggtggt agcagtgcat ggtcacaccc
     5041 tcagtttgag aaatgactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     5101 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5161 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5221 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5281 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5341 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5401 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5461 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5521 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5581 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5641 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     5701 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     5761 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     5821 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5881 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5941 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6001 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6061 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6121 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6181 caagagacag gatgaggatc gtttcgcatg accgagtaca agcccacggt gcgcctcgcc
     6241 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     6301 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     6361 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     6421 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     6481 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     6541 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     6601 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     6661 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     6721 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     6781 tgcatgaccc gcaagcccgg tgcctgagcg ggactctggg gttcgaaatg accgaccaag
     6841 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     6901 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     6961 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     7021 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7081 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     7141 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7201 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7261 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7321 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7381 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7441 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7501 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7561 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7621 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7681 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7741 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7801 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7861 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7921 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7981 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8041 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt
     8101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9301 acgtcgacgg atcgggagat ctcccgatcc cctatggt
//
