LOCUS       V039293                10981 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039293
VERSION     V039293
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1597..6567
                     /label="IQGAP1(NM_003870)"
                     /note="IQGAP1(NM_003870)"
                     /gene="IQGAP1"
     CDS             6574..6663
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     polyA_signal    6743..6967
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      7013..7441
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7455..7784
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7851..8450
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    8624..8745
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8794..8810)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8818..8834
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8842..8872)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8887..8908
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9196..9781)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9952..10812)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10813..10917)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag aagcttatgt ccgccgcaga cgaggttgac
     1621 gggctgggcg tggcccggcc gcactatggc tctgtcctgg ataatgaaag acttactgca
     1681 gaggagatgg atgaaaggag acgtcagaac gtggcttatg agtacctttg tcatttggaa
     1741 gaagcgaaga ggtggatgga agcatgccta ggggaagatc tgcctcccac cacagaactg
     1801 gaggaggggc ttaggaatgg ggtctacctt gccaaactgg ggaacttctt ctctcccaaa
     1861 gtagtgtccc tgaaaaaaat ctatgatcga gaacagacca gatacaaggc gactggcctc
     1921 cactttagac acactgataa tgtgattcag tggttgaatg ccatggatga gattggattg
     1981 cctaagattt tttacccaga aactacagat atctatgatc gaaagaacat gccaagatgt
     2041 atctactgta tccatgcact cagtttgtac ctgttcaagc taggcctggc ccctcagatt
     2101 caagacctat atggaaaggt tgacttcaca gaagaagaaa tcaacaacat gaagactgag
     2161 ttggagaagt atggcatcca gatgcctgcc tttagcaaga ttgggggcat cttggctaat
     2221 gaactgtcag tggatgaagc cgcattacat gctgctgtta ttgctattaa tgaagctatt
     2281 gaccgtagaa ttccagccga cacatttgca gctttgaaaa atccgaatgc catgcttgta
     2341 aatcttgaag agcccttggc atccacttac caggatatac tttaccaggc taagcaggac
     2401 aaaatgacaa atgctaaaaa caggacagaa aactcagaga gagaaagaga tgtttatgag
     2461 gagctgctca cgcaagctga aattcaaggc aatataaaca aagtcaatac attttctgca
     2521 ttagcaaata tcgacctggc tttagaacaa ggagatgcac tggccttgtt cagggctctg
     2581 cagtcaccag ccctggggct tcgaggactg cagcaacaga atagcgactg gtacttgaag
     2641 cagctcctga gtgataaaca gcagaagaga cagagtggtc agactgaccc cctgcagaag
     2701 gaggagctgc agtctggagt ggatgctgca aacagtgctg cccagcaata tcagagaaga
     2761 ttggcagcag tagcactgat taatgctgca atccagaagg gtgttgctga gaagactgtt
     2821 ttggaactga tgaatcccga agcccagctg ccccaggtgt atccatttgc cgccgatctc
     2881 tatcagaagg agctggctac cctgcagcga caaagtcctg aacataatct cacccaccca
     2941 gagctctctg tcgcagtgga gatgttgtca tcggtggccc tgatcaacag ggcattggaa
     3001 tcaggagatg tgaatacagt gtggaagcaa ttgagcagtt cagttactgg tcttaccaat
     3061 attgaggaag aaaactgtca gaggtatctc gatgagttga tgaaactgaa ggctcaggca
     3121 catgcagaga ataatgaatt cattacatgg aatgatatcc aagcttgcgt ggaccatgtg
     3181 aacctggtgg tgcaagagga acatgagagg attttagcca ttggtttaat taatgaagcc
     3241 ctggatgaag gtgatgccca aaagactctg caggccctac agattcctgc agctaaactt
     3301 gagggagtcc ttgcagaagt ggcccagcat taccaagaca cgctgattag agcgaagaga
     3361 gagaaagccc aggaaatcca ggatgagtca gctgtgttat ggttggatga aattcaaggt
     3421 ggaatctggc agtccaacaa agacacccaa gaagcacaga agtttgcctt aggaatcttt
     3481 gccattaatg aggcagtaga aagtggtgat gttggcaaaa cactgagtgc ccttcgctcc
     3541 cctgatgttg gcttgtatgg agtcatccct gagtgtggtg aaacttacca cagtgatctt
     3601 gctgaagcca agaagaaaaa actggcagta ggagataata acagcaagtg ggtgaagcac
     3661 tgggtaaaag gtggatatta ttattaccac aatctggaga cccaggaagg aggatgggat
     3721 gaacctccaa attttgtgca aaattctatg cagctttctc gggaggagat ccagagttct
     3781 atctctgggg tgactgccgc atataaccga gaacagctgt ggctggccaa tgaaggcctg
     3841 atcaccaggc tgcaggctcg ctgccgtgga tacttagttc gacaggaatt ccgatccagg
     3901 atgaatttcc tgaagaaaca aatccctgcc atcacctgca ttcagtcaca gtggagagga
     3961 tacaagcaga agaaggcata tcaagatcgg ttagcttacc tgcgctccca caaagatgaa
     4021 gttgtaaaga ttcagtccct ggcaaggatg caccaagctc gaaagcgcta tcgagatcgc
     4081 ctgcagtact tccgggacca tataaatgac attatcaaaa tccaggcttt tattcgggca
     4141 aacaaagctc gggatgacta caagactctc atcaatgctg aggatcctcc tatggttgtg
     4201 gtccgaaaat ttgtccacct gctggaccaa agtgaccagg attttcagga ggagcttgac
     4261 cttatgaaga tgcgggaaga ggttatcacc ctcattcgtt ctaaccagca gctggagaat
     4321 gacctcaatc tcatggatat caaaattgga ctgctagtga aaaataagat tacgttgcag
     4381 gatgtggttt cccacagtaa aaaacttacc aaaaaaaata aggaacagtt gtctgatatg
     4441 atgatgataa ataaacagaa gggaggtctc aaggctttga gcaaggagaa gagagagaag
     4501 ttggaagctt accagcacct gttttattta ttgcaaacca atcccaccta tctggccaag
     4561 ctcatttttc agatgcccca gaacaagtcc accaagttca tggactctgt aatcttcaca
     4621 ctctacaact acgcgtccaa ccagcgagag gagtacctgc tcctgcggct ctttaagaca
     4681 gcactccaag aggaaatcaa gtcgaaggta gatcagattc aagagattgt gacaggaaat
     4741 cctacggtta ttaaaatggt tgtaagtttc aaccgtggtg cccgtggcca gaatgccctg
     4801 agacagatct tggccccagt cgtgaaggaa attatggatg acaaatctct caacatcaaa
     4861 actgaccctg tggatattta caaatcttgg gttaatcaga tggagtctca gacaggagag
     4921 gcaagcaaac tgccctatga tgtgacccct gagcaggcgc tagctcatga agaagtgaag
     4981 acacggctag acagctccat caggaacatg cgggctgtga cagacaagtt tctctcagcc
     5041 attgtcagct ctgtggacaa aatcccttat gggatgcgct tcattgccaa agtgctgaag
     5101 gactcgttgc atgagaagtt ccctgatgct ggtgaggatg agctgctgaa gattattggt
     5161 aacttgcttt attatcgata catgaatcca gccattgttg ctcctgatgc ctttgacatc
     5221 attgacctgt cagcaggagg ccagcttacc acagaccaac gccgaaatct gggctccatt
     5281 gcaaaaatgc ttcagcatgc tgcttccaat aagatgtttc tgggagataa tgcccactta
     5341 agcatcatta atgaatatct ttcccagtcc taccagaaat tcagacggtt tttccaaact
     5401 gcttgtgatg tcccagagct tcaggataaa tttaatgtgg atgagtactc tgatttagta
     5461 accctcacca aaccagtaat ctacatttcc attggtgaaa tcatcaacac ccacactctc
     5521 ctgttggatc accaggatgc cattgctccg gagcacaatg atccaatcca cgaactgctg
     5581 gacgacctcg gcgaggtgcc caccatcgag tccctgatag gggaaagctc tggcaattta
     5641 aatgacccaa ataaggaggc actggctaag acggaagtgt ctctcaccct gaccaacaag
     5701 ttcgacgtgc ctggagatga gaatgcagaa atggatgctc gaaccatctt actgaataca
     5761 aaacgtttaa ttgtggatgt catccggttc cagccaggag agaccttgac tgaaatccta
     5821 gaaacaccag ccaccagtga acaggaagca gaacatcaga gagccatgca gagacgtgct
     5881 atccgtgatg ccaaaacacc tgacaagatg aaaaagtcaa aatctgtaaa ggaagacagc
     5941 aacctcactc ttcaagagaa gaaagagaag atccagacag gtttaaagaa gctaacagag
     6001 cttggaaccg tggacccaaa gaacaaatac caggaactga tcaacgacat tgccagggat
     6061 attcggaatc agcggaggta ccgacagagg agaaaggccg aactagtgaa actgcaacag
     6121 acatacgctg ctctgaactc taaggccacc ttttatgggg agcaggtgga ttactataaa
     6181 agctatatca aaacctgctt ggataactta gccagcaagg gcaaagtctc caaaaagcct
     6241 agggaaatga aaggaaagaa aagcaaaaag atttctctga aatatacagc agcaagacta
     6301 catgaaaaag gagttcttct ggaaattgag gacctgcaag tgaatcagtt taaaaatgtt
     6361 atatttgaaa tcagtccaac agaagaagtt ggagacttcg aagtgaaagc caaattcatg
     6421 ggagttcaaa tggagacttt tatgttacat tatcaggacc tgctgcagct acagtatgaa
     6481 ggagttgcag tcatgaaatt atttgataga gctaaagtaa atgtcaacct cctgatcttc
     6541 cttctcaaca aaaagttcta cgggaaggga tccgaacaaa aactaatatc agaggaagat
     6601 ttggaacaga aactaatcag tgaagaagat ttagagcaaa agttaatttc cgaggaggac
     6661 ttataggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     6721 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6781 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6841 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6901 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6961 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     7021 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7081 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7141 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7201 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7261 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7321 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7381 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7441 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7501 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7561 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7621 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7681 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7741 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7801 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgaccgagt
     7861 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     7921 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     7981 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8041 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     8101 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     8161 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     8221 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8281 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     8341 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8401 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gcgggactct
     8461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     8521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8761 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8821 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8881 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8941 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9001 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9721 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    10021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    10081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    10141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    10201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    10261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    10321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    10381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    10441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    10501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    10561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    10621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    10681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10921 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
    10981 t
//
