LOCUS V046858 6098 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V046858 VERSION V046858 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1717 /label="HOXA5(NM_019102)" /note="HOXA5(NM_019102)" /gene="HOXA5" CDS 1724..1813 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 1860..2084 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2130..2558 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2572..2901 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2968..3567 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" polyA_signal 3741..3862 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3911..3927) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3935..3951 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3959..3989) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4004..4025 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4313..4898) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5069..5929) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5930..6034) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg agctcttatt ttgtaaactc attttgcggt cgctatccaa atggcccgga 961 ctaccagttg cataattatg gagatcatag ttccgtgagc gagcaattca gggactcggc 1021 gagcatgcac tccggcaggt acggctacgg ctacaatggc atggatctca gcgtcggccg 1081 ctcgggctcc ggccactttg gctccggaga gcgcgcccgc agctacgctg ccagcgccag 1141 cgcggcgccc gccgagccca ggtacagcca gccggccacg tccacgcact ctcctcagcc 1201 cgatccgctg ccctgctccg ccgtggcccc ctcgcccggc agcgacagcc accacggcgg 1261 gaaaaactcc ctaagcaact ccagcggcgc ctcggccgac gccggcagca cccacatcag 1321 cagcagagag ggggttggca cggcgtccgg agccgaggag gacgcccctg ccagcagcga 1381 gcaggcgagt gcgcagagcg agccgagccc ggcgccgccc gcccaacccc agatctaccc 1441 ctggatgcgc aagctgcaca taagtcatga caacataggc ggcccggaag gcaaaagggc 1501 ccggacggcc tacacgcgct accagaccct ggagctggag aaggagttcc acttcaaccg 1561 ttacctgacc cgcagaagga ggattgaaat agcacatgct ctttgcctct ccgagagaca 1621 aattaaaatc tggttccaaa accggagaat gaagtggaaa aaagataata agctgaaaag 1681 catgagcatg gccgcggcag gaggggcctt ccgtcccgat atcgaacaaa aactaatatc 1741 agaggaagat ttggaacaga aactaatcag tgaagaagat ttagagcaaa agttaatttc 1801 cgaggaggac ttatagctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac 1861 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 1921 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 1981 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 2041 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 2101 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 2161 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 2221 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 2281 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 2341 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 2401 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 2461 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 2521 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag 2581 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2641 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 2701 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 2761 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 2821 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 2881 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat 2941 caagagacag gatgaggatc gtttcgcatg accgagtaca agcccacggt gcgcctcgcc 3001 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 3061 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa 3121 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 3181 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 3241 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 3301 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 3361 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 3421 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 3481 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 3541 tgcatgaccc gcaagcccgg tgcctgagcg ggactctggg gttcgaaatg accgaccaag 3601 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg 3661 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc 3721 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca 3781 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 3841 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg 3901 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 3961 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4021 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 4141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4201 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4261 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 4321 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 4381 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 4441 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 4501 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 4561 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 4621 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 4681 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 4741 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 4801 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt 4861 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4921 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4981 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 5041 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 5101 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 5161 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 5221 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 5281 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 5341 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 5401 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5461 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5521 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5581 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5641 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 5701 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5761 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 5821 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5881 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 5941 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 6001 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 6061 acgtcgacgg atcgggagat ctcccgatcc cctatggt //