LOCUS       V029739                 8038 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029739
VERSION     V029739
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..3666
                     /label="ARHGEF1(NM_004706)"
                     /note="ARHGEF1(NM_004706)"
                     /gene="ARHGEF1"
     CDS             3673..3699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3703..3729
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3733..3759
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3800..4024
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4070..4498
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4512..4841
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4908..5507
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     polyA_signal    5681..5802
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5851..5867)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5875..5891
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5899..5929)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5944..5965
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6253..6838)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7009..7869)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7870..7974)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggaagact tcgcccgagg ggcggcctcc
      961 ccaggcccct cccggcctgg cctggttccc gtcagcatca tcggggctga ggatgaggat
     1021 tttgagaacg agctggagac aaactcagaa gagcaaaaca gccagttcca gagcctggag
     1081 caggtgaagc ggcgcccagc ccacctcatg gccctcctgc agcacgtggc cctgcagttt
     1141 gagccaggac ccctgctttg ctgtctgcat gccgacatgc tgggctcact gggccccaag
     1201 gaggccaaga aggccttcct ggacttctac cacagcttcc tggagaagac agcggttctc
     1261 cgggtgccgg tccctcccaa cgtcgccttt gaacttgacc gcactagggc tgacctcatc
     1321 tccgaggatg tccagcggcg gttcgtgcag gaggtggtgc aaagccagca ggtagccgtg
     1381 ggccggcagc tggaggactt ccgttccaag cggctcatgg gcatgacgcc ctgggagcag
     1441 gagctggccc agctggaggc ttgggttggg cgggaccgag ccagctacga ggcccgggag
     1501 cggcacgtgg cggagcggct gctcatgcac ctggaggaga tgcaacatac catctctacc
     1561 gacgaagaaa agagtgctgc cgtggtcaac gccattggcc tgtacatgcg ccaccttggg
     1621 gtgcggacca agagtggaga caagaagtcg gggaggaact tcttccggaa aaaggtgatg
     1681 gggaaccggc ggtcggacga gcctgccaag accaagaagg ggctgagcag catcctggat
     1741 gccgcccgct ggaaccgggg agagccccag gttccagatt ttcgacacct caaagcagag
     1801 gttgatgccg agaagccagg tgctacagac cggaagggag gcgtggggat gccctctcgg
     1861 gaccggaata tcggggctcc tgggcaggac acccctggag tctctctgca ccctctgtcc
     1921 ctggacagcc cagaccggga accaggtgct gacgcccccc tggagctggg ggactcatcc
     1981 ccgcagggcc caatgagcct ggagtccttg gcgcccccag agagtaccga cgagggggcc
     2041 gaaaccgaga gccccgagcc tggagatgag ggggagccgg ggcggtcggg actggagctt
     2101 gaaccagaag agcctcccgg ctggcgggaa ctcgtccccc cagacaccct gcacagcctg
     2161 cccaagagcc aggtgaagcg gcaggaggtc atcagcgagc tgctggtgac agaggcggcc
     2221 cacgtgcgca tgctgcgggt gctgcacgac ctcttcttcc agcccatggc agaatgcctg
     2281 ttcttcccct tggaggagct gcagaacatc ttccccagcc tggacgagct catcgaggtg
     2341 cattccctgt tcctcgatcg cctgatgaag cggaggcagg agagtggcta cctcatcgag
     2401 gagatcggag acgtgctgct ggcccggttt gatggtgctg agggctcctg gttccagaaa
     2461 atctcctccc gcttctgcag ccgccagtca tttgccttag agcagctcaa agccaagcaa
     2521 cgcaaggacc ctcggttctg tgccttcgtg caggaagctg agagccgccc gcggtgccgc
     2581 cgcctgcagc tgaaggacat gatccccacg gagatgcagc ggctgaccaa gtaccccctg
     2641 ctcctgcaga gcatcgggca gaacacagaa gagcccacag aacgggagaa agtggagctg
     2701 gcagccgagt gctgccggga aattctacac cacgtcaacc aagccgtgcg tgacatggag
     2761 gacctgctga ggctcaagga ctatcagcgg cgcctggact tgtcccacct tcggcagagc
     2821 agcgacccta tgctgagcga gttcaagaac ctggacatca ccaagaagaa attggtccac
     2881 gagggcccac tgacgtggcg ggtgactaag gacaaggcag tggaggtgca tgtgctgctg
     2941 ctggacgacc tgctgctgct gctccagcgc caggacgagc ggctgctgct caagtcccat
     3001 agccggacac tgacgcccac gcccgatggc aagaccatgc tgcggcccgt gctgcggctc
     3061 acctccgcca tgacccgcga ggtggccacc gatcacaaag ccttctacgt cctttttacc
     3121 tgggaccagg aggcccagat atacgagctg gtggcacaga ctgtgtcgga gcggaaaaac
     3181 tggtgtgctc tcatcactga gactgccgga tccctgaaag tccctgcccc tgcctctcgc
     3241 cctaagcccc ggcccagccc gagcagcacc cgagaacccc tcctcagcag ctctgagaac
     3301 ggcaatggtg gccgagagac gtctccagct gatgcccgga ccgagagaat cctcagtgac
     3361 ctcctgccct tctgcagacc aggccccgag ggccagctcg ctgccacggc ccttcggaaa
     3421 gtgctgtccc tgaagcagct tctgtttccg gcggaggaag acaatggggc ggggcctcct
     3481 cgagatgggg atggggtccc agggggcggc cccctgagcc cagcacggac ccaggaaatc
     3541 caggagaacc tgctcagctt ggaggagacc atgaagcagc tggaggagtt ggaggaggaa
     3601 ttttgccgcc tgagacccct cctgtctcag cttgggggga actctgtccc ccagcctggc
     3661 tgcactctcg agtacccata cgacgtacct gattatgcaa cttatcctta tgatgtacct
     3721 gattatgcta gctatcctta tgatgtccca gactacgcat agtctagagg gcccgtttaa
     3781 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3841 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3901 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3961 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4021 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4501 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4561 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4621 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4681 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4741 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4801 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4861 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg accgagtaca
     4921 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     4981 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     5041 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5101 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5161 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5221 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5281 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5341 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5401 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5461 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgagcg ggactctggg
     5521 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5581 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5641 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5701 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5761 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5821 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5941 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6001 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6061 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6781 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6841 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6901 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6961 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7921 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7981 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggt
//
