LOCUS       V028342                 7972 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028342
VERSION     V028342
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..3022
                     /label="Ppargc1a(NM_031347)"
                     /note="Ppargc1a(NM_031347)"
                     /gene="Ppargc1a"
     CDS             3035..3100
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3141..3365
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        3381..3904
                     /label="mCMV promoter"
     CDS             3918..4637
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4760..4881
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4888..5343)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5370..5474
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5476..5833
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5868..6662
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6894..6941
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7270..7858
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatggct tgggacatgt gcagccaaga
      661 ctctgtatgg agtgacatag agtgtgctgc cctggttggt gaggaccagc ctctttgccc
      721 agatcttcct gaacttgacc tttctgaact tgatgtgaat gacctggaca cagacagctt
      781 tctgggtgga ttgaagtggt gtagtgacca atcagaaatc atatccaacc agtacaacaa
      841 tgagcccgcg aacatattcg agaagataga tgaagagaat gaggcaaact tgctagcggt
      901 cctcacagag acactggaca gtctcccggt ggatgaagac ggattgccct catttgatgc
      961 actgacagat ggagacgtga ccactgacaa cgaggccagt ccttcctcca tgcctgacgg
     1021 cacccctccc cctcaggagg cagaagagcc gtctctactt aagaagctct tactggcgcc
     1081 agccaacact cagctcagct acaatgaatg cagcggtctt agcactcaga accatgcaaa
     1141 ccacacccac aggatcagaa caaaccctgc cattgttaag accgagaatt catggagcaa
     1201 taaagcaaag agcatttgtc aacagcaaaa gccacaaaga cgtccctgct cagagcttct
     1261 caagtatctg accacaaacg atgaccctcc tcacaccaaa cccacagaga acagaaacag
     1321 cagcagagac aaatgtgctt ccaaaaagaa gtcccataca caaccgcagt cgcaacatgc
     1381 tcaagccaaa ccaacaactt tatctcttcc tctgacccca gagtcaccaa atgaccccaa
     1441 gggttcccca tttgagaaca agactattga gcgaacctta agtgtggaac tctctggaac
     1501 tgcaggccta actcctccca cgactcctcc tcataaagcc aaccaagata accctttcaa
     1561 ggcttcgcca aagctgaagc cctcttgcaa gaccgtggtg ccaccaccaa caaagagggc
     1621 ccggtacagt gagtgttctg gtacccaagg cagccactcc accaagaaag ggccggagca
     1681 atctgagtta tacgcacaac tcagcaagtc ctcagtgctc agccgaggac acgaggaaag
     1741 gaagactaaa cggcccagtc tacggctgtt tggtgatcat gactactgtc agtcagtcaa
     1801 ttccaaaacg gatatactca ttaacatatc acaggagctc caagactcta gacaactaga
     1861 cttcaaagat gcctcctgtg actggcaggg gcacatctgt tcttccacag attcaagcca
     1921 gtgctacctg agagagactt tggaggccag caagcaggtc tctccttgca gcaccagaaa
     1981 acagctccaa gaccaggaaa tccgagctga gctgaacaag cacttcggtc atcccagtca
     2041 agctgttttt gacgacaaag tagacaagac cagtgaacta cgggatggca acttcagtaa
     2101 tgaacaattc tccaaactac ctgtgtttat aaattcagga ctagccatgg atggcctatt
     2161 tgatgacagt gaagatgaaa atgataaact gagctaccct tgggatggca cgcagtccta
     2221 ttcattgttc gatgtgtcgc cttcttgctc ttcctttaac tctccgtgtc gagattcagt
     2281 gtcaccaccg aaatccttat tttctcaaag accccaaagg atgcgctctc gttcgagatc
     2341 cttttctcga cacaggtcgt gttcccgatc accatattcc aggtcaagat caaggtcccc
     2401 aggcagtaga tcctcttcaa gatcctgtta ctattatgaa tcaagccact acagacaccg
     2461 cacacatcgc aattctccct tgtatgtgag atcacgttca aggtcaccct acagccgtag
     2521 gcccaggtat gacagctatg aagccaatga gcacgaaagg ctcaagaggg acgaataccg
     2581 cagagagtat gagaagcggg agtctgaaag ggccaagcag agagagaggc agaagcagaa
     2641 agcaattgaa gagcgccgtg tgatttacgt tggtaaaatc agacctgaca caacgcggac
     2701 agaactgaga gaccgctttg aagtttttgg tgaaattgag gaatgcaccg taaatctgcg
     2761 ggatgatgga gacagctatg gtttcattac ctaccgttac acctgtgacg ctttcgctgc
     2821 tcttgagaat ggatatactt tacgcaggtc gaatgaaact gacttcgagc tgtacttttg
     2881 tggacggaag caatttttca agtctaacta tgcagaccta gattcaaact cagacgattt
     2941 tgaccctgct tccaccaaga gcaagtatga ctctctggat tttgatagtt tactgaagga
     3001 agctcagaga agcttgcgca ggaagcttac gcgtgactac aaagaccatg acggtgatta
     3061 taaagatcat gacatcgact acaaggatga cgatgacaag tgaggatccg ggcccgttta
     3121 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     3181 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     3241 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     3301 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     3361 tatggtctag agatatactg agtcattagg gactttccaa tgggttttgc ccagtacata
     3421 aggtcaatag gggtgaatca acaggaaagt cccattggag ccaagtacac tgagtcaata
     3481 gggactttcc attgggtttt gcccagtaca aaaggtcaat agggggtgag tcaatgggtt
     3541 tttcccatta ttggcacgta cataaggtca ataggggtga gtcattgggt ttttccagcc
     3601 aatttaatta aaacgccatg tactttccca ccattgacgt caatgggcta ttgaaactaa
     3661 tgcaacgtga cctttaaacg gtactttccc atagctgatt aatgggaaag taccgttctc
     3721 gagccaatac acgtcaatgg gaagtgaaag ggcagccaaa acgtaacacc gccccggttt
     3781 tcccctggaa attccatatt ggcacgcatt ctattggctg agctgcgttc tacgtgggta
     3841 taagaggcgc gaccagcgtc ggtaccgtcg cagtcttcgg tctgaccacc gtagaacgca
     3901 gatcaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3961 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4021 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4081 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4141 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4201 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4261 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4321 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4381 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4441 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4501 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     4561 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4621 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     4681 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     4741 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     4801 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     4861 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     4921 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     4981 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5041 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5101 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5161 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     5221 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     5281 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     5341 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5401 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5461 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     5521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5581 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     5641 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     5701 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     5761 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     5821 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     5881 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     5941 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6001 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     6061 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6121 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6181 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6241 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6301 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6361 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     6421 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6481 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6541 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6601 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6661 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     6721 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6781 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     6841 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     6901 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     6961 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     7021 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     7081 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     7141 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7201 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7261 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7321 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7381 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7441 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7501 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7561 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7621 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7681 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7741 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7801 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7861 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7921 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
