LOCUS       V014980                 7555 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014980
VERSION     V014980
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             941..2944
                     /gene="PIP5K1C"
                     /label="Phosphatidylinositol 4-phosphate 5-kinase type-1
                     gamma"
                     /note="Phosphatidylinositol 4-phosphate 5-kinase type-1
                     gamma from Homo sapiens. Accession#: O60331"
     CDS             2951..2971
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             2972..2995
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             3032..3055
                     /label="Strep-Tag II"
                     /note="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
     CDS             3056..3082
                     /label="9xHis"
                     /note="9xHis affinity tag"
     polyA_signal    3155..3379
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3425..3853
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3867..4196
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4263..5054
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5231..5364
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5401..5417)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5425..5441)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5449..5479)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5494..5515)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5803..6388)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6562..7419)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7420..7524)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggagctgg aggtaccgga
      961 cgaggcggag agcgctgagg cgggggccgt gccctcggag gcggcgtggg cggcagagag
     1021 cggggcggcg gcaggtttgg ctcagaagaa ggcggcccca acagaggttc tgtccatgac
     1081 ggcacagccg ggccctggcc atgggaagaa gttgggccat cgaggtgtgg acgcatccgg
     1141 cgaaaccacc tacaagaaga ccacctcctc caccctgaag ggtgccatcc agctgggcat
     1201 cggctacacc gtgggccacc tgagctccaa gcccgaacgc gacgtgctca tgcaggactt
     1261 ctacgtggtg gagagcatct tcttccccag cgaaggcagc aacctcaccc ccgcccacca
     1321 cttccaggac ttccgcttca agacctatgc acctgtcgcc ttccgctact tccgggagct
     1381 ctttgggatc cggccagatg attacttgta ctccctgtgc aatgagccgc tgatcgagct
     1441 gtccaacccg ggcgccagtg gctccctctt ctacgtcacc agcgacgacg agttcatcat
     1501 caagaccgtc atgcacaagg aggccgagtt cctgcagaag ctgctccctg gctactacat
     1561 gaacctcaac cagaacccgc ggacgctgct gcccaagttc tatgggctgt actgcgtgca
     1621 gtcggggggc aagaacatcc gcgtcgtggt catgaacaac atcctgcccc gcgtggtcaa
     1681 gatgcacctc aagttcgacc tcaagggctc cacctacaag cggcgcgcca gcaagaagga
     1741 gaaggagaag agcttcccca cctacaagga cctggacttc atgcaggaca tgcccgaggg
     1801 gctcctgctg gacgccgaca ccttcagcgc cctggtcaag acgctgcagc gggactgcct
     1861 ggtcctggaa agtttcaaga tcatggacta cagcctgctg ctgggcgtgc acaacatcga
     1921 ccagcacgag cgcgagcggc aggcgcaggg cgcccagagc acctcagatg agaagcggcc
     1981 tgtgggccag aaggcgctct actccacggc catggagtcc atccagggtg gcgccgcgcg
     2041 cggggaggcc atcgaatcgg atgacacgat gggcgggatc cccgctgtga acggccgcgg
     2101 ggagcggctg ctgctgcaca ttggcatcat cgacatcctg cagtcctaca ggttcatcaa
     2161 gaaactggag cacacctgga aggccctcgt ccacgatggg gacacggtgt ccgtccaccg
     2221 ccccagcttc tatgccgagc gctttttcaa gttcatgagc aacacggtct ttcggaagaa
     2281 ctcctccctg aagtcctcgc cctccaagaa ggggcgcggc ggagccttgc tagctgtgaa
     2341 accgctgggg cccaccgctg ccttctcggc cagccagatc cctagcgagc gggaggaggc
     2401 ccagtacgac ctgcgggggg cccgcagcta ccccacgctg gaggacgaag gccggcccga
     2461 cctcctgccc tgcacgccac cttctttcga agaagccact acagcctcca ttgccacgac
     2521 tctgtcatcc acatccctct ccattcctga gcggtccccc tcggagacgt cggagcagcc
     2581 gcggtacagg cggcgcacac agtcgtctgg acaggatggc aggccgcagg aggagccacc
     2641 cgcggaagag gatctgcagc agattacagt gcaggtggag cctgcgtgca gcgtggagat
     2701 tgtggtcccc aaagaggagg acgcaggggt ggaggcttcc ccggccggtg cctctgctgc
     2761 tgttgaagta gaaactgcca gccaggcctc agacgaggag ggcgcacctg ccagccaggc
     2821 ctcggacgag gaggacgcgc ccgccaccga catctacttt cccaccgatg agaggagctg
     2881 ggtgtactcc ccgctccact atagcgccca ggcccccccg gcctccgacg gcgagagcga
     2941 cacagaattc gaaaacctgt attttcaggg ctggagccac ccgcagttcg agaaaggtgg
     3001 aggttccgga ggtggatcgg gaggtggatc gtggagccac ccgcagttcg aaaaacacca
     3061 ccaccatcac catcatcacc accactgaga tatccagcac agtggcggcc gctcgagtct
     3121 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3361 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3421 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3781 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3841 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3901 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4021 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4081 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4141 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4201 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     4261 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4321 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4381 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4441 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4501 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4561 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4621 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4681 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4741 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     4801 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4861 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4921 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4981 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5041 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5101 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5161 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5221 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5341 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5401 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5461 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5521 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5581 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     6361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
