LOCUS       Exported                9255 bp DNA     circular SYN 06-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2024 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..433
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        434..637
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        679..697
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             747..767
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             768..4868
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4926..4946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4971..6461
                     /product="Gag-Pol polyprotein from MMLV"
                     /label=Gag-Pol polyprotein
     CDS             6507..6527
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6537..6563
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6572..6589
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6618..6842
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6913..6929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6937..6953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6961..6991)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7006..7027
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7315..7903)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8074..8934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8935..9039)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
       61 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      121 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      181 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      241 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      301 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      361 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      421 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      481 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      541 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      601 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
      661 ctagagatcc gcggccgcta atacgactca ctatagggag agccgccacc atgaaacgga
      721 cagccgacgg aagcgagttc gagtcaccaa agaagaagcg gaaagtcgac aagaagtaca
      781 gcatcggcct ggacatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
      841 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
      901 acctgatcgg agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga
      961 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     1021 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     1081 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     1141 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     1201 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     1261 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     1321 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     1381 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagaaagctg gaaaatctga
     1441 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc
     1501 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     1561 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     1621 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     1681 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     1741 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     1801 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg
     1861 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     1921 ccgaggaact gctcgtgaag ctgaagagag aggacctgct gcggaagcag cggaccttcg
     1981 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     2041 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     2101 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     2161 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     2221 cttccgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     2281 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca
     2341 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     2401 aggccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     2461 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     2521 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     2581 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     2641 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     2701 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     2761 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     2821 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     2881 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     2941 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     3001 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     3061 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     3121 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     3181 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     3241 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     3301 ttctgaagga cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca
     3361 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc
     3421 tgctgaacgc caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag
     3481 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     3541 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     3601 atgacaagct gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     3661 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     3721 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     3781 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     3841 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     3901 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     3961 acggcgaaac cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag
     4021 tgctgagcat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     4081 gcaaagagtc tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact
     4141 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     4201 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     4261 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     4321 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     4381 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     4441 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     4501 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc
     4561 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     4621 atctggacaa agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg
     4681 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     4741 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     4801 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     4861 gaggtgactc cggcggaagc tctggtggca gcaagcggac cgccgacggc tctgaattcg
     4921 agagccctaa gaagaaaaga aaggtgagcg gaggctctag cggcggaagc accctgaaca
     4981 ttgaagacga gtatagactg catgaaacaa gcaaggaacc cgacgtgtcc ctgggctcca
     5041 cctggctgtc cgactttccc caggcctggg ccgagacagg aggaatgggc ctggccgtgc
     5101 ggcaggcacc cctgatcatc cctctgaagg ccacctctac acccgtgagc atcaagcagt
     5161 accctatgtc tcaggaggcc agactgggca tcaagcctca catccagagg ctgctggacc
     5221 agggcatcct ggtgccatgc cagagcccct ggaacacacc actgctgccc gtgaagaagc
     5281 caggcaccaa tgactataga cccgtgcagg atctgagaga ggtgaacaag agggtggagg
     5341 atatccaccc caacgtgccc aacccttaca atctgctgtc cggcctgccc ccttctcacc
     5401 agtggtatac agtgctggac ctgaaggatg ccttcttttg tctgagactg caccctacca
     5461 gccagccact gttcgccttt gagtggaggg accctgagat gggcatctct ggccagctga
     5521 cctggacacg cctgcctcag ggcttcaaga atagcccaac actgttttgc gaggccctgc
     5581 accgcgacct ggcagatttc cggatccagc acccagatct gatcctgctg cagtactacg
     5641 acgatctgct gctggccgcc accagcgagc tggattgcca gcagggaaca cgcgccctgc
     5701 tgcagaccct gggaaacctg ggatataggg catccgccaa gaaggcccag atctgtcaga
     5761 agcaggtgaa gtacctgggc tatctgctga aggagggcca gagatggctg acagaggcca
     5821 ggaaggagac agtgatgggc cagccaacac ccaagacccc aagacagctg agggagttcc
     5881 tgggcaaagc aggattttgc aggctgttca tcccaggatt cgcagagatg gcagcacctc
     5941 tgtacccact gaccaagccg ggcaccctgt ttaattgggg ccctgaccag cagaaggcct
     6001 atcaggagat caagcaggcc ctgctgacag caccagccct gggcctgcca gacctgacca
     6061 agcctttcga gctgtttgtg gatgagaagc agggctacgc caagggcgtg ctgacccaga
     6121 agctgggacc atggagacgg cccgtggcct atctgtccaa gaagctggac ccagtggcag
     6181 caggatggcc accatgcctg aggatggtgg cagcaatcgc cgtgctgaca aaggatgccg
     6241 gcaagctgac catgggacag ccactggtca tcctggcacc acacgcagtg gaggccctgg
     6301 tgaagcagcc tccagatcgc tggctgtcta acgcccggat gacacactac caggccctgc
     6361 tgctggacac cgatcgcgtg cagtttggcc ctgtggtggc cctgaatcca gccaccctgc
     6421 tgcctctgcc agaggagggc ctgcagcaca actgtctgga ctctggcggc tcaaaaagaa
     6481 ccgccgacgg cagcgaattc gagtctccca agaagaagag gaaagtcggc tctggccctg
     6541 ccgctaagag agtgaagctg gactaaccgg tcatcatcac catcaccatt gagtttaaac
     6601 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     6661 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagaa
     6721 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     6781 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     6841 ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt
     6901 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     6961 caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact
     7021 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7081 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7141 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7201 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7261 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7321 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7381 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7441 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7501 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7561 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7621 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7681 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7741 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     7801 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     7861 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     7921 ttctacgggg tctgacactc agtggaacga aaactcacgt taagggattt tggtcatgag
     7981 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8041 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8101 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8161 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8221 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8281 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8341 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8401 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8461 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8521 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8581 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8641 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8701 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     8761 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     8821 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     8881 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     8941 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9001 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9061 acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac
     9121 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     9181 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     9241 catgaagaat ctgct
//
