LOCUS       V046942                 8722 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046942
VERSION     V046942
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             635..3772
                     /label="OGT(NM_181672)"
                     /note="OGT(NM_181672)"
                     /gene="OGT"
     CDS             3785..3850
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    3891..4115
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     promoter        4131..4654
                     /label="mCMV promoter"
     CDS             4668..5387
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    5510..5631
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5638..6093)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6120..6224
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        6226..6583
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6618..7412
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7644..7691
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      8020..8608
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct ccgaattcgc caccatggcg tcttccgtgg gcaacgtggc
      661 cgacagcaca gaaccaacga aacgtatgct ttccttccaa gggttagctg agttggcaca
      721 tcgagaatat caggcaggag attttgaggc agctgagaga cactgcatgc agctctggag
      781 acaagagcca gacaatactg gtgtgctttt attactttca tctatacact tccagtgtcg
      841 aaggctggac agatctgctc actttagcac tctggcaatt aaacagaacc cccttctggc
      901 agaagcttat tcgaatttgg ggaatgtgta caaggaaaga gggcagttgc aggaggcaat
      961 tgagcattat cgacatgcat tgcgtctcaa acctgatttc atcgatggtt atattaacct
     1021 ggcagccgcc ttggtagcag cgggtgacat ggaaggggca gtacaagctt acgtctctgc
     1081 tcttcagtac aatcctgatt tgtactgtgt tcgcagtgac ctggggaacc tgctcaaagc
     1141 cctgggtcgc ttggaagaag ccaaggcatg ttatttgaaa gcaattgaga cgcaaccgaa
     1201 ctttgcagta gcttggagta atcttggctg tgttttcaat gcacaagggg aaatttggct
     1261 tgcaattcat cactttgaaa aggctgtcac ccttgaccca aactttctgg atgcttatat
     1321 caatttagga aatgtcttga aagaggcacg catttttgac agagctgtgg cagcttatct
     1381 tcgtgcccta agtttgagtc caaatcacgc agtggtgcac ggcaacctgg cttgtgtata
     1441 ctatgagcaa ggcctgatag atctggcaat agacacctac aggcgggcta tcgaactaca
     1501 accacatttc cctgatgctt actgcaacct agccaatgct ctcaaagaga agggcagtgt
     1561 tgctgaagca gaagattgtt ataatacagc tctccgtctg tgtcccaccc atgcagactc
     1621 tctgaataac ctagccaata tcaaacgaga acagggaaac attgaagagg cagttcgctt
     1681 gtatcgtaaa gcattagaag tcttcccaga gtttgctgct gcccattcaa atttagcaag
     1741 tgtactgcag cagcagggaa aactgcagga agctctgatg cattataagg aggctattcg
     1801 aatcagtcct acctttgctg atgcctactc taatatggga aacactctaa aggagatgca
     1861 ggatgttcag ggagccttgc agtgttatac gcgtgccatc caaattaatc ctgcatttgc
     1921 agatgcacat agcaatctgg cttccattca taaggattca gggaatattc cagaagccat
     1981 agcttcttac cgcacggctc tgaaacttaa gcctgatttt cctgatgctt attgtaactt
     2041 ggctcattgc ctgcagattg tctgtgattg gacagactat gatgagcgaa tgaagaagtt
     2101 ggtcagtatt gtggctgacc agttagagaa gaataggttg ccttctgtgc atcctcatca
     2161 tagtatgcta tatcctcttt ctcatggctt caggaaggct attgctgaga ggcacggcaa
     2221 cctgtgctta gataagatta atgttcttca taaaccacca tatgaacatc caaaagactt
     2281 gaagctcagt gatggtcggc tgcgtgtagg atatgtgagt tccgactttg ggaatcatcc
     2341 tacttctcac cttatgcagt ctattccagg catgcacaat cctgataaat ttgaggtgtt
     2401 ctgttatgcc ctgagcccag acgatggcac aaacttccga gtgaaggtga tggcagaagc
     2461 caatcatttc attgatcttt ctcagattcc atgcaatgga aaagcagctg atcgcatcca
     2521 tcaggatgga attcatatcc ttgtaaatat gaatggctat actaagggcg ctcgaaatga
     2581 gctttttgct ctcaggccag ctcctattca ggcaatgtgg ctgggatacc ctgggacgag
     2641 tggtgcgctt ttcatggatt atattatcac tgatcaggaa acttcgccag ctgaagttgc
     2701 tgagcagtat tccgagaaat tggcttatat gccccacact ttttttattg gtgatcatgc
     2761 taatatgttc cctcacctga agaaaaaagc agtcatcgat tttaagtcca atgggcacat
     2821 ttatgacaat cggatagttc tgaatggcat cgacctcaaa gcatttcttg atagtctacc
     2881 agatgtgaaa attgtcaaga tgaagtgtcc tgatggagga gacaatgcag atagcagtaa
     2941 cacagctctt aatatgcctg ttattcctat gaatactatt gcagaagcag ttattgaaat
     3001 gattaaccga ggacagattc aaataacaat taatggattc agtattagca atggactggc
     3061 aactactcag atcaacaata aggctgcaac tggagaggag gttccccgta ccattattgt
     3121 aaccacccgt tctcagtacg ggttaccaga agatgccatc gtatactgta actttaatca
     3181 gttgtataaa attgaccctt ctactttgca gatgtgggca aacattctga agcgtgttcc
     3241 caatagtgta ctctggctgt tgcgttttcc agcagtagga gaacctaata ttcaacagta
     3301 tgcacaaaac atgggcctgc cccagaaccg tatcattttt tcacctgttg ctcctaaaga
     3361 ggaacacgtc aggagaggcc agctggctga tgtctgcttg gacactccac tctgtaatgg
     3421 gcacaccaca gggatggatg tcctctgggc agggaccccc atggtgacta tgccaggaga
     3481 gactcttgct tctcgagttg cagcatccca gctcacttgc ttaggttgtc ttgagcttat
     3541 tgctaaaaac agacaagaat atgaagacat agctgtgaag ctgggaactg atctagaata
     3601 cctgaagaaa gttcgtggca aagtctggaa gcaaagaata tctagccctc tgttcaacac
     3661 caaacaatac acaatggaac tagagcggct ctatctacag atgtgggagc attatgcagc
     3721 tggcaacaaa cctgaccaca tgattaagcc tgttgaagtc actgagtcag caaagcttac
     3781 gcgtgactac aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga
     3841 cgatgacaag tgaggatccg ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     3901 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3961 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     4021 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     4081 tagcaggcat gctggggatg cggtgggctc tatggtctag agatatactg agtcattagg
     4141 gactttccaa tgggttttgc ccagtacata aggtcaatag gggtgaatca acaggaaagt
     4201 cccattggag ccaagtacac tgagtcaata gggactttcc attgggtttt gcccagtaca
     4261 aaaggtcaat agggggtgag tcaatgggtt tttcccatta ttggcacgta cataaggtca
     4321 ataggggtga gtcattgggt ttttccagcc aatttaatta aaacgccatg tactttccca
     4381 ccattgacgt caatgggcta ttgaaactaa tgcaacgtga cctttaaacg gtactttccc
     4441 atagctgatt aatgggaaag taccgttctc gagccaatac acgtcaatgg gaagtgaaag
     4501 ggcagccaaa acgtaacacc gccccggttt tcccctggaa attccatatt ggcacgcatt
     4561 ctattggctg agctgcgttc tacgtgggta taagaggcgc gaccagcgtc ggtaccgtcg
     4621 cagtcttcgg tctgaccacc gtagaacgca gatcaccggt cgccaccatg gtgagcaagg
     4681 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     4741 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     4801 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     4861 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     4921 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     4981 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5041 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5101 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     5161 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     5221 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5281 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5341 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgcgact
     5401 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5461 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5521 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5581 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     5641 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5701 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5761 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5821 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5881 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5941 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     6001 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     6061 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     6121 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     6181 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     6241 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6301 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6361 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6421 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6481 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     6541 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     6601 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6661 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6721 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6781 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6841 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6901 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6961 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7021 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7081 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7141 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7201 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7261 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7321 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7381 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     7441 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     7501 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7561 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     7621 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     7681 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     7741 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     7801 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     7861 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     8041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     8101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     8161 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     8221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     8281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     8341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     8401 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     8461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     8521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8641 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8701 taaccgtatt accgccatgc at
//
