LOCUS       V041366                 6578 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041366
VERSION     V041366
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             4..30
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             58..2520
                     /label="Ntrk2(NM_001025074)"
                     /note="Ntrk2(NM_001025074)"
                     /gene="Ntrk2"
     CDS             2623..2874
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /transl_table=1
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
                     /label="VC155"
     polyA_signal    2889..3023
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(3134..3150)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    3158..3174
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(3182..3212)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    3227..3248
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3536..4124)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(4295..5155)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(5156..5260)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5734..5750
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     enhancer        5777..6080
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        6081..6284
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          6422..6518
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
ORIGIN
        1 atgtacccat acgatgttcc agattacgct cttatggcca tggaggcccg aattcgtatg
       61 tcgccctggc tgaagtggca tggacccgcc atggcgcggc tctggggctt atgcctgctg
      121 gtcttgggct tctggagggc ctctctcgcc tgcccgacgt cctgcaaatg cagttccgct
      181 aggatttggt gtactgagcc ttctccaggc atcgtggcat tcccgaggtt ggaacctaac
      241 agcgttgacc cggagaacat cacggaaatt ctcattgcaa accagaaaag gctagaaatc
      301 atcaatgaag atgacgttga agcttacgtg gggctgagaa accttacaat tgtggattcc
      361 ggcttaaagt ttgtggctta caaagcgttt ctgaaaaaca gcaacctgcg gcacataaat
      421 ttcacacgaa acaagctgac gagtttgtcc aggagacatt tccgccacct tgacttgtct
      481 gacctgatcc tgacgggtaa tccgttcacg tgctcctgcg acatcatgtg gctcaagact
      541 ctccaggaga ctaaatccag ccccgacact caggatttgt actgcctcaa tgagagcagc
      601 aagaacatgc ccctggcgaa cctgcagata cccaattgtg gtctgccatc tgcacgtctg
      661 gctgctccta acctcaccgt ggaggaagga aagtctgtga ccctttcctg cagtgtgggg
      721 ggtgacccac tccccacctt gtactgggac gttgggaatt tggtttccaa gcacatgaat
      781 gaaacaagcc acacacaggg ctccttaagg ataacgaaca tttcatctga tgacagtgga
      841 aagcaaatct cttgtgtggc agaaaacctt gtaggagaag atcaagattc tgtgaacctc
      901 actgtgcatt ttgcgccaac tatcacgttt ctcgagtctc caacctcaga tcaccactgg
      961 tgcattccat tcactgtgag aggcaacccc aagcctgcgc ttcagtggtt ctacaatggg
     1021 gccatactga atgagtccaa gtacatctgt actaagatcc acgtcaccaa tcacacggag
     1081 taccatggct gcctccagct ggataacccc actcatatga ataacggaga ctacaccctg
     1141 atggccaaga acgagtatgg gaaggatgag agacagatct ccgctcactt catgggccgg
     1201 cctggagtcg actacgagac aaacccaaat taccctgaag tcctctatga agactggacc
     1261 acgccaactg acattgggga tactacgaac aaaagtaatg aaatcccctc cacggatgtt
     1321 gctgaccaaa gcaatcggga gcatctctcg gtctatgccg tggtggtgat tgcatctgtg
     1381 gtgggattct gcctgctggt gatgttgctc ctgctcaagt tggcgagaca ttccaagttt
     1441 ggcatgaaag gcccagcttc ggtcatcagc aacgacgatg actctgccag ccccctccac
     1501 cacatctcca atgggagtaa cactccatct tcttcggagg gcggtcccga cgctgtcatt
     1561 attggaatga ccaagattcc tgttattgaa aacccccagt actttggcat caccaacagt
     1621 cagctcaagc cagacacatt tgttcagcat atcaagagac acaacatcgt tctgaagagg
     1681 gaacttgggg aaggagcctt cgggaaagtt ttccttgccg agtgctacaa cctctgccca
     1741 gagcaggata agatcctggt ggctgtgaag acgctgaagg acgccagcga caatgcacgc
     1801 aaggactttc atcgggaagc tgagctgctg accaacctcc agcacgagca cattgtcaag
     1861 ttctacggtg tctgtgtgga gggcgaccca ctcatcatgg tctttgagta catgaagcac
     1921 ggggacctca acaagttcct tagggcacac gggcccgacg cagtgctgat ggcagagggt
     1981 aacccgccca cagagctgac gcagtcgcag atgctgcaca tcgctcagca aatcgcagca
     2041 ggtatggtct acctggcgtc ccaacacttt gtgcaccgtg acctggccac ccggaactgc
     2101 ctggtgggag agaacctgct ggtgaaaatt ggggactttg ggatgtcccg agatgtgtac
     2161 agcaccgact actatcgggt cggtggccac acaatgttgc ccatccgatg gatgcctcca
     2221 gagagcatca tgtacaggaa attcaccacc gagagcgacg tctggagcct gggcgttgtg
     2281 ttgtgggaga tcttcaccta cggcaagcag ccctggtatc agctatcgaa caatgaggtg
     2341 atagagtgca tcacccaggg aagagtcctt cagcggcctc gaacgtgtcc ccaggaggtg
     2401 tatgagctca tgcttggatg ctggcagcgg gaaccacaca cccggaagaa catcaagagc
     2461 atccacaccc tccttcagaa cttggccaag gcatctcccg tctacctgga tatcctaggc
     2521 ggtggaggcg gatcaggagg cggtggatct ggaggcggtg gatcagaatt ccgtccggcg
     2581 tgcaaaatcc cgaacgacct gaaacagaaa gtcatgaacc acgacaagca gaagaacggc
     2641 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     2701 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2761 ctgagctacc agtccaaact gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2821 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcg
     2881 gccgcgggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     2941 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3001 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3061 agggggaggt gtgggaggtt ttttcggatc ctctagagtc gatctgcagg catgctagct
     3121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     3181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     3241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     3301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     3361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     3421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     3481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     3541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     3601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3841 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3901 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3961 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     4021 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     4081 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     4141 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     4201 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     4261 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     4321 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     4381 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     4441 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     4501 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     4561 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     4621 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4681 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     4741 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     4801 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     4861 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     4921 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4981 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5041 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5101 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5161 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5221 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5281 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5341 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     5401 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     5461 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     5521 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     5581 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5641 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     5701 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagttcgagc
     5761 ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5821 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5881 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5941 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     6001 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     6061 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     6121 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     6181 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     6241 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     6301 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     6361 ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag aaagttaact
     6421 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg gtgcaaatca
     6481 aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc
     6541 tgctctaaaa gctgcggaat tgtacccgcg ggcccacc
//
