LOCUS       .                       7385 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1319..1372
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             1373..2905
                     /label="VSV-G"
                     /note="vesicular stomatitis virus G glycoprotein"
     polyA_signal    2952..3176
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3222..3650
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3664..3993
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4060..4851
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5028..5161
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5198..5214)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5222..5238)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5246..5276)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5291..5312)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5600..6185)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6359..7216)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(7217..7321)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg accaacatga gctgcgccta cgagctgatc aagagcctgc ccgccaagct
      961 ggaacagctg gcccaggaaa cccaggccac catccagacc ctgatgatcg ccgaccccaa
     1021 cgtgaacaag gacctgcggg ccttctgcga gttcctgacc gtgcagcacc agcgggccta
     1081 cagagccacc aacagcctgc tgattaagcc cagagtggcc gctgccctga gaggcgagga
     1141 actggatctg ggagaggccg atgtggccgc tagagtgcgg cagctgaaac agcagctggc
     1201 cgagctggaa atggaaatca agcccggcca tcagcaggtg gcccaggtgt ccggaagaag
     1261 aaaagccgcc gctgccgctc ctgtggctca gctgggaaga gtgggagtcg tgaacgagga
     1321 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaagtg
     1381 ccttttgtac ttagcctttt tattcattgg ggtgaattgc aagttcacca tagtttttcc
     1441 acacaaccaa aaaggaaact ggaaaaatgt tccttctaat taccattatt gcccgtcaag
     1501 ctcagattta aattggcata atgacttaat aggcacagcc ttacaagtca aaatgcccaa
     1561 gagtcacaag gctattcaag cagacggttg gatgtgtcat gcttccaaat gggtcactac
     1621 ttgtgatttc cgctggtatg gaccgaagta tataacacat tccatccgat ccttcactcc
     1681 atctgtagaa caatgcaagg aaagcattga acaaacgaaa caaggaactt ggctgaatcc
     1741 aggcttccct cctcaaagtt gtggatatgc aactgtgacg gatgccgaag cagtgattgt
     1801 ccaggtgact cctcaccatg tgctggttga tgaatacaca ggagaatggg ttgattcaca
     1861 gttcatcaac ggaaaatgca gcaattacat atgccccact gtccgtaact ctacaacctg
     1921 gcattctgac tataaggtca aagggctatg tgattctaac ctcatttcca tggacatcac
     1981 cttcttctca gaggacggag agctatcatc cctgggaaag gagggcacag ggttcagaag
     2041 taactacttt gcttatgaaa ctggaggcaa ggcctgcaaa atgcaatact gcaagcattg
     2101 gggagtcaga ctcccatcag gtgtctggtt cgagatggct gataaggatc tctttgctgc
     2161 agccagattc cctgaatgcc cagaagggtc aagtatctct gctccatctc agacctcagt
     2221 ggatgtaagt ctaattcagg acgttgagag gatcttggat tattccctct gccaagaaac
     2281 ctggagcaaa atcagagcgg gtcttccaat ctctccagtg gatctcagct atcttgctcc
     2341 taaaaaccca ggaaccggtc ctgctttcac cataatcaat ggtaccctaa aatactttga
     2401 gaccagatac atcagagtcg atattgctgc tccaatcctc tcaagaatgg tcggaatgat
     2461 cagtggaact accacagaaa gggaactgtg ggatgactgg gcaccatatg aagacgtgga
     2521 aattggaccc aatggagttc tgaggaccag ttcaggatat aagtttcctt tatacatgat
     2581 tggacatggt atgttggact ccgatcttca tcttagctca aaggctcagg tgttcgaaca
     2641 tcctcacatt caagacgctg cttcgcaact tcctgatgat gagagtttat tttttggtga
     2701 tactgggcta tccaaaaatc caatcgagct tgtagaaggt tggttcagta gttggaaaag
     2761 ctctattgcc tcttttttct ttatcatagg gttaatcatt ggactattct tggttctccg
     2821 agttggtatc catctttgca ttaaattaaa gcacaccaag aaaagacaga tttatacaga
     2881 catagagatg aaccgacttg gaaagtgact cgagtctaga gggcccgttt aaacccgctg
     2941 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3001 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3061 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3121 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3181 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3241 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3301 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3361 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3421 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3481 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3541 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3601 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     3661 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3721 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3781 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3841 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3901 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     3961 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4021 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4081 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4141 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4201 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4261 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4321 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4381 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4441 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4501 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4561 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4621 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4681 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     4741 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     4801 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     4861 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     4921 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     4981 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5041 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5101 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5161 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5221 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5281 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5341 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5401 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5461 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5521 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5581 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5641 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5701 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5761 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5821 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5881 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5941 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6001 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6061 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6121 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7321 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     7381 atggt
//
