LOCUS       pCMV-Nlk(mouse)-HA      5406 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022677
VERSION     V022677
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        27..330
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        331..534
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     intron          672..768
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
                     /label="SV40 intron"
     CDS             864..2444
                     /label="Nlk(NM_008702)"
                     /note="Nlk(NM_008702)"
                     /gene="Nlk"
     CDS             2445..2471
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2475..2501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2505..2531
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    2545..2679
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(2790..2806)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2814..2830
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2838..2868)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    2883..2904
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(3192..3780)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(3951..4811)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(4812..4916)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     5390..5406
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
       61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
      541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
      661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
      721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
      781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt
      841 cgaccgagat ctctcgagcc accatgtctc tttgtggcac aagagccaac gcaaaaatga
      901 tggcggctta caatggcggt acatctgcag ctgcagcagg tcaccaccac caccatcatc
      961 accaccttcc acaccttcct cctcctcacc ttcaccacca ccaccaccct caacaccacc
     1021 ttcatccggg gtcggctgct gccgtacacc cagtccagca gcatacgtct tcggcagctg
     1081 cggcagccgc cgcagcagct gcagccgcag ccatgttaaa ccctgggcaa caacagccat
     1141 atttcccatc cccggcaccg ggtcaggctc ctgggccagc cgcagcagcc ccagctcagg
     1201 tacaggctgc tgcagctgct acagttaagg cgcaccatca tcagcactcg catcatccgc
     1261 aacagcagct ggacattgag ccggatagac caattggata tggagccttt ggcgttgtct
     1321 ggtcagtaac agatccaaga gatggaaaga gagtagcact caaaaagatg cccaacgtct
     1381 tccagaatct ggtctcttgc aaaagagtct tccgggaatt gaagatgttg tgttttttta
     1441 aacatgataa tgtactctct gcccttgaca tactccaacc tccacacatt gactattttg
     1501 aagaaatata tgttgtcaca gaattgatgc agagtgatct acataaaatt atcgtctctc
     1561 ctcagccact cagctcggat catgtcaaag tttttcttta tcagattttg cgaggtttga
     1621 aatatctcca ttcagctggc attttgcatc gagacattaa gccagggaac ctccttgtga
     1681 acagcaattg tgttctaaag atttgtgatt ttggattggc cagagtggag gaattggatg
     1741 aatcccgtca tatgactcag gaagtagtta ctcagtatta ccgggctccg gagatcctga
     1801 tgggcagccg tcattacagc aatgctatcg acatctggtc tgtcgggtgc atctttgcag
     1861 agctactggg ccgaagaata ttgtttcagg cgcagagtcc cattcagcag ttggatttga
     1921 tcacagatct gttgggcaca ccatcactgg aagcaatgag gacagcttgt gaaggtgcta
     1981 aggcacacat actcaggggt cctcataaac agccatctct tcctgtgctc tacaccctct
     2041 ccagccaggc cacacatgaa gctgttcacc tgctttgcag gatgttggtc tttgatccat
     2101 caaaaagaat atccgctaag gatgccttag cccaccccta cctagatgaa gggcggctga
     2161 gataccatac gtgtatgtgc aagtgctgct tttccacctc aacggggaga gtctacacca
     2221 gtgactttga gcctgtcacc aaccccaaat ttgatgacac ctttgagaag aacctcagct
     2281 ctgtccgaca ggttaaagaa attatccacc agttcattct ggaacagcag aaaggaaaca
     2341 gagtgcctct ctgcatcaac ccgcagtccg ctgcctttaa gagctttatt agttccaccg
     2401 tcgctcagcc gtctgagatg ccgccgtctc ctctggtgtg ggagtaccca tacgacgtac
     2461 ctgattatgc aacttatcct tatgatgtac ctgattatgc tagctatcct tatgatgtcc
     2521 cagactacgc atagcggccg cggggatcca gacatgataa gatacattga tgagtttgga
     2581 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     2641 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     2701 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt cggatcctct agagtcgatc
     2761 tgcaggcatg ctagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2821 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2881 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2941 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     3001 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3061 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3121 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3181 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3241 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3301 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3361 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3421 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3481 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3541 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3601 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3661 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3721 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3781 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3841 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3901 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3961 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4021 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4081 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4141 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4201 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4261 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4321 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4381 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4441 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4501 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4561 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4621 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4681 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4741 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4801 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4861 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4921 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4981 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     5041 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     5101 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     5161 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     5221 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     5281 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     5341 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     5401 gccagt
//
