LOCUS       V020991                 6192 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020991
VERSION     V020991
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..2847
                     /label="ZDHHC14(NM_024630)"
                     /note="ZDHHC14(NM_024630)"
                     /gene="ZDHHC14"
     polyA_signal    2980..3101
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3108..3563)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3590..3694
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3696..4053
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4088..4882
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5114..5161
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5490..6078
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatgcctc ccggcggcgg cgggcccatg aaagactgcg agtacagcca gatcagcacc
     1441 cacagctcct cccccatgga gtcgccccac aagaagaaga aaatcgcggc ccggaggaaa
     1501 tgggaggtgt tcccgggaag aaacaagttc ttctgtaacg ggaggatcat gatggcccgg
     1561 cagacgggcg tcttctacct gacgctcgtc ctcatcctgg tcactagcgg actcttcttc
     1621 gccttcgact gtccgtacct ggcggtgaaa atcacccctg ccatccctgc agtcgctggc
     1681 atcctgttct tctttgtgat ggggaccctg ctccgcacca gcttcagcga ccccggagtc
     1741 ctcccacgag ccacgcctga tgaagccgcc gatctggaaa ggcaaataga tatcgcaaac
     1801 ggcaccagtt caggggggta ccgcccgcct cccagaacca aagaagtcat catcaatggc
     1861 cagaccgtga aacttaaata ctgtttcacc tgcaagattt tccggccccc tcgcgcctcc
     1921 cattgcagcc tttgtgataa ctgcgtagaa cggtttgatc accactgtcc ctgggtaggc
     1981 aactgtgtgg ggaaaagaaa ctacagattt ttttatatgt ttattttatc tctgtctttt
     2041 ctgacagtct ttatatttgc attcgttatc acccacgtca ttcttcgttc acagcaaaca
     2101 ggattcctaa atgcccttaa ggacagtcct gcaagcgtcc tggaggctgt ggtgtgcttc
     2161 ttctctgtct ggtccatcgt tggcctctca ggattccaca cctacttgat cagctccaac
     2221 cagacaacaa atgaggacat taaaggatcc tggtcaaata aaagaggtaa agaaaattac
     2281 aatccctaca gctacggaaa tatctttacc aactgctgtg ttgccctgtg tgggcccatc
     2341 tcaccaagcc tgatcgacag aagagggtac atccagcccg acacgccgca gccagcagca
     2401 ccctccaatg gcatcaccat gtacggggcc acgcagtcac agagtgacat gtgcgaccaa
     2461 gaccagtgca ttcagagcac caaattcgtt ttgcaggctg cagccacgcc cctgctgcag
     2521 agcgagccca gcctcaccag cgacgagctg cacctgcccg ggaagcctgg cctgggcacg
     2581 ccctgcgcca gcctcacact gggcccgccc acaccgcccg cctccatgcc caacctcgcc
     2641 gaggccacgc tcgcggacgt gatgccccgg aaagatgagc acatgggcca ccagttcctg
     2701 acgcccgatg aggcgccctc gccccccagg ctactggcgg cgggcagccc cctggcgcac
     2761 agccgcacca tgcacgtgct gggcctggcc agccaggact ccctgcatga ggactctgtg
     2821 cgcggcctgg tgaagctcag ctccgtgtga ggatccaccg gatctagata actgatcata
     2881 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     2941 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3061 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
     3121 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3181 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3241 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3301 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3361 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3421 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3481 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3541 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3601 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3661 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3721 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3781 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3841 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3901 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3961 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4021 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4081 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4141 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4201 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4261 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4321 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4381 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4441 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4501 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4561 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4621 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4681 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4741 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     4801 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     4861 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     4921 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4981 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5041 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     5101 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     5161 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5221 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5281 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5341 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5401 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5461 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5521 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5581 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5641 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5701 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5761 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5821 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5881 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5941 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6001 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6061 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6121 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6181 accgccatgc at
//
