LOCUS       V040113                 5616 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V040113
VERSION     V040113
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     regulatory      607..616
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             616..681
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     CDS             688..2271
                     /label="YARS1(NM_003680)"
                     /note="YARS1(NM_003680)"
                     /gene="YARS1"
     polyA_signal    2404..2525
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2532..2987)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3014..3118
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3120..3477
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3512..4306
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4538..4585
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4914..5502
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgac
      661 tacaaggatg acgatgacaa gaagcttatg ggggacgctc ccagccctga agagaaactg
      721 caccttatca cccggaacct gcaggaggtt ctgggggaag agaagctgaa ggagatactg
      781 aaggagcggg aacttaaaat ttactgggga acggcaacca cgggcaaacc acatgtggct
      841 tactttgtgc ccatgtcaaa gattgcagac ttcttaaagg cagggtgtga ggtaacaatt
      901 ctgtttgcgg acctccacgc atacctggat aacatgaaag ccccatggga acttctagaa
      961 ctccgagtca gttactatga gaatgtgatc aaagcaatgc tggagagcat tggtgtgccc
     1021 ttggagaagc tcaagttcat caaaggcact gattaccagc tcagcaaaga gtacacacta
     1081 gatgtgtaca gactctcctc cgtggtcaca cagcacgatt ccaagaaggc tggagctgag
     1141 gtggtaaagc aggtggagca ccctttgctg agtggcctct tataccccgg actgcaggct
     1201 ttggatgaag agtatttaaa agtagatgcc caatttggag gcattgatca gagaaagatt
     1261 ttcacctttg cagagaagta cctccctgca cttggctatt caaaacgggt ccatctgatg
     1321 aatcctatgg ttccaggatt aacaggcagc aaaatgagct cttcagaaga ggagtccaag
     1381 attgatctcc ttgatcggaa ggaggatgtg aagaaaaaac tgaagaaggc cttctgtgag
     1441 ccaggaaatg tggagaacaa tggggttctg tccttcatca agcatgtcct ttttcccctt
     1501 aagtccgagt ttgtgatcct acgagatgag aaatggggtg gaaacaaaac ctacacagct
     1561 tacgtggacc tggaaaagga ctttgctgct gaggttgtac atcctggaga cctgaagaat
     1621 tctgttgaag tcgcactgaa caagttgctg gatccaatcc gggaaaagtt taatacccct
     1681 gccctgaaaa aactggccag cgctgcctac ccagatccct caaagcagaa gccaatggcc
     1741 aaaggccctg ccaagaattc agaaccagag gaggtcatcc catcccggct ggatatccgt
     1801 gtggggaaaa tcatcactgt ggagaagcac ccagatgcag acagcctgta tgtagagaag
     1861 attgacgtgg gggaagctga accacggact gtggtgagcg gcctggtaca gttcgtgccc
     1921 aaggaggaac tgcaggacag gctggtagtg gtgctgtgca acctgaaacc ccagaagatg
     1981 agaggagtcg agtcccaagg catgcttctg tgtgcttcta tagaagggat aaaccgccag
     2041 gttgaacctc tggaccctcc ggcaggctct gctcctggtg agcacgtgtt tgtgaagggc
     2101 tatgaaaagg gccaaccaga tgaggagctc aagcccaaga agaaagtctt cgagaagttg
     2161 caggctgact tcaaaatttc tgaggagtgc atcgcacagt ggaagcaaac caacttcatg
     2221 accaagctgg gctccatttc ctgtaaatcg ctgaaagggg ggaacattag ctagggatcc
     2281 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     2341 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     2401 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     2461 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     2521 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2581 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2641 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2701 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2761 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2821 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2881 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2941 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     3001 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     3061 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     3121 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3181 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3241 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3301 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3421 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     3481 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     3541 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3601 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     3661 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     3721 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     3781 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     3841 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     3901 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     3961 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4021 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4081 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4141 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4201 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4261 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     4321 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     4381 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4441 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     4501 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     4561 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     4621 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     4681 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     4741 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     4801 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4861 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4921 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4981 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5041 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5101 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5161 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5221 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5281 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5341 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5401 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5461 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5521 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5581 cctgattctg tggataaccg tattaccgcc atgcat
//
