LOCUS       V043397                 7542 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043397
VERSION     V043397
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1323
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from
                     mRuby2, with improved brightness and photostability (Bajar
                     et al., 2016)"
                     /transl_table=1
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
                     /label="mRuby3"
     CDS             1354..4182
                     /label="WDR3(NM_006784)"
                     /note="WDR3(NM_006784)"
                     /gene="WDR3"
     polyA_signal    4330..4451
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4458..4913)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4940..5044
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5046..5403
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5438..6232
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6464..6511
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6840..7428
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatca aggaaaatat gcgtatgaag
      661 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc
      721 agaccgtacg agggagtgca aaccatgagg atcaaagtca tcgagggagg acccctgcca
      781 tttgcctttg acattcttgc cacgtcgttc atgtatggca gccgtacctt tatcaagtac
      841 ccggccgaca tccctgattt ctttaaacag tcctttcctg agggttttac ttgggaaaga
      901 gttacgagat acgaagatgg tggagtcgtc accgtcacgc aggacaccag ccttgaggat
      961 ggcgagctcg tctacaacgt caaggtcaga ggggtaaact ttccctccaa tggtcccgtg
     1021 atgcagaaga agaccaaggg ttgggagcct aatacagaga tgatgtatcc agcagatggt
     1081 ggtctgagag gatacactga catcgcactg aaagttgatg gtggtggcca tctgcactgc
     1141 aacttcgtga caacttacag gtcaaaaaag accgtcggga acatcaagat gcccggtgtc
     1201 catgccgttg atcaccgcct ggaaaggatc gaggagagtg acaatgaaac ctacgtagtg
     1261 caaagagaag tggcagttgc caaatacagc aaccttggtg gtggcatgga cgagctgtac
     1321 aagtccggac tcagatctcg agctcaagct tccatggggc tcaccaagca gtacctacgc
     1381 tatgttgcta gtgcggtctt tggcgttatc ggcagccaaa aaggtaatat tgtctttgtg
     1441 acacttcgtg gtgagaaagg acgttatgtg gcagtaccag cttgtgaaca cgttttcatc
     1501 tgggacttaa ggaaaggaga gaagattctt atccttcagg ggcttaaaca agaagttact
     1561 tgcttatgcc cctccccaga tgggctacac ttagctgttg ggtatgagga tgggtcgatc
     1621 cgaatcttca gtctcctgag tggggaagga aatgtgacct tcaatggtca caaagcagct
     1681 atcactacct tgaagtatga tcagctagga ggcagactgg catctgggtc caaggacaca
     1741 gatattattg tatgggatgt gatcaatgaa agtggtctgt accgtctaaa ggggcacaag
     1801 gatgccatca cacaagcatt gtttctacga gaaaagaacc tgctagttac tagtgggaaa
     1861 gataccatgg tgaaatggtg ggaccttgat actcagcact gctttaaaac aatggttggc
     1921 caccggactg aggtatgggg gttggttctg ttgtcagaag aaaagcgact catcactggg
     1981 gcctcagaca gtgaactgag ggtatgggac atagcttatc tgcaagagat tgaagacccg
     2041 gaagaaccag accccaagaa aatcaaagga tcttctcctg gaatacaaga tactcttgag
     2101 gcagaggatg gtgcctttga gacggatgaa gcccctgagg atcgaatcct ttcatgcaga
     2161 aaagctggtt ccataatgcg ggaaggaaga gacagagttg taaaccttgc agtcgacaag
     2221 acaggcagga ttcttgcttg ccatggaact gactctgtgc tagaattgtt ttgtatcctt
     2281 tccaaaaagg aaattcagaa gaaaatggat aagaagatga agaaagctag aaagaaagca
     2341 aaattacatt ctagcaaagg agaggaggaa gatcctgagg ttaatgttga aatgagtctg
     2401 caagatgaaa tccagcgggt gactaatata aaaacttctg ccaaaatcaa gtcctttgac
     2461 ttgattcatt cacctcacgg agagttaaag gctgtcttcc tgctgcagaa caacctggtg
     2521 gaattgtatt cactgaatcc atccttgcct actcctcagc ctgtcaggac aagcagaatc
     2581 actattgggg gtcatcgcag tgatgtgcgg actttgtcat tcagctcaga caatattgct
     2641 gttctttcag ctgcagctga ttccattaaa atatggaaca ggtctacact gcagtgtatt
     2701 cgcacaatga cctgtgaata tgcactttgc tcattctttg tacctggtga tagacaggta
     2761 gtcataggaa caaagacagg gaagctgcag ctttatgact tggcttcagg gaatctgctg
     2821 gagacaatag atgcacatga tggagctttg tggtccatgt ccctctctcc agatcagcgt
     2881 ggctttgtga caggtggtgc agataaatct gtcaaattct gggattttga gttagtgaaa
     2941 gatgaaaata gtacccaaaa gagactttct gtgaagcaaa cccgaacttt gcaactagat
     3001 gaagatgttc tgtgtgtcag ttactctccc aatcaaaagc tattggctgt gtctttgctg
     3061 gactgtactg tgaaaatttt ctacgttgat actttaaagt tttttctgtc actgtatgga
     3121 cacaaactgc ctgttatatg catggacatc tctcatgatg gagcactcat agcaactggc
     3181 tccgctgata ggaatgtgaa aatctggggt ttggactttg gggactgcca caagtctctc
     3241 tttgcacatg atgacagtgt gatgtaccta cagtttgtac ccaagtctca cctcttcttc
     3301 actgccggaa aagatcataa gattaaacag tgggatgcag acaaatttga acacatacag
     3361 actctggagg gtcatcacca ggaaatatgg tgtttggctg taagccccag tggagactat
     3421 gttgtatcat cgtcccatga caaatctctg agactttggg agagaacaag ggagcctctt
     3481 attcttgagg aagaaaggga gatggaaaga gaagcagaat atgaggagag tgtggccaaa
     3541 gaagaccaac cagcagttcc aggagagact caaggtgaca gttactttac tggaaagaaa
     3601 actattgaaa cagtgaaagc agctgagagg attatggagg ctattgagtt gtaccgagaa
     3661 gaaactgcaa aaatgaagga acacaaagcc atttgtaaag ctgcagggaa agaggttcca
     3721 cttcccagca accccatcct aatggcttat ggcagtatct caccttcagc ttatgtatta
     3781 gagattttta aagggatcaa gtcgagtgag ctggaagaat ctctacttgt gctgcctttc
     3841 tcttatgtcc cagacattct taaactcttt aacgaattca ttcagctggg ctctgatgtt
     3901 gaacttatat gccggtgcct cttcttcctc cttaggattc actttggaca gatcactagc
     3961 aatcaaatgc ttgtgccagt gatagaaaaa ttaagggaaa caactatttc aaaagtcagc
     4021 caagtccggg atgttatcgg cttcaatatg gctggtcttg attatctcaa gagggaatgc
     4081 gaggcaaaaa gtgaagttat gttttttgct gatgctacta gccacttgga agagaagaag
     4141 aggaagagga aaaagaggga gaagttgatt ctaacgttga cttagggtac cgcgggcccg
     4201 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     4261 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4321 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4381 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4441 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     4501 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4561 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4621 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4681 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4741 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4801 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4861 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5041 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5101 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5161 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5221 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5281 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5341 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5401 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     5461 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     5521 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5581 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     5641 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5701 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5761 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5821 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5881 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5941 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     6001 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     6061 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     6121 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     6181 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     6241 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     6301 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6361 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     6421 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     6481 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     6541 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     6601 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6661 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6721 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6781 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6841 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6901 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6961 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7021 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7081 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7141 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7201 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7261 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7321 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7381 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7441 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     7501 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
