LOCUS       V028135                 6123 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028135
VERSION     V028135
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..2778
                     /label="WASHC1(NM_182905)"
                     /note="WASHC1(NM_182905)"
                     /gene="WASHC1"
     polyA_signal    2911..3032
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3039..3494)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3521..3625
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3627..3984
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4019..4813
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5045..5092
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5421..6009
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatgactc ctgtgaggat gcagcactcc ctggcaggtc agacctatgc cgtgcccttc
     1441 atccagccag acctgcggcg agaggaggcc gtccagcaga tggcggatgc cctgcagtac
     1501 ctgcagaagg tctctggaga catcttcagc aggatctccc agcaggtaga gcagagccgg
     1561 agccaggtgc aggccattgg agagaaggtc tccttggccc aggccaagat tgagaagatc
     1621 aagggcagca agaaggccat caaggtgttc tccagtgcca agtaccctgc tccagggcgc
     1681 ctgcaggaat atggctccat cttcacgggc gcccaggacc ctggcctgca gagacgcccc
     1741 cgccacagga tccagagcaa gcaccgcccc ctggacgagc gggccctgca ggagaagctg
     1801 aaggactttc ctgtgtgcgt gagcaccaag ccggagcccg aggacgatgc agaagaggga
     1861 cttgggggtc ttcccagcaa catcagctct gtcagctcct tgctgctctt caacaccacc
     1921 gagaacctgt acaagaagta tgtcttcctg gaccccctgg ctggtgctgt aacaaagacc
     1981 catgtgatgc tgggggcaga gacagaggag aagctgtttg atgccccctt gtccatcagc
     2041 aagagagagc agctggaaca gcaggtccca gagaactact tctatgtgcc agacctgggc
     2101 caggtgcctg agatccatgt tccatcctac ctgcctgacc tgcccggcat tgccaacgac
     2161 ctcatgtaca gtgccgacct gggccccggc attgccccct ctgcccctgg caccattcca
     2221 gaactgccca ccttccacac tgaggtagcc gagcctctca aggtagacct acaagatggg
     2281 gtactaacac cacccccacc gcccccacca ccacccccag ctcctgaggt gctggccagt
     2341 gcacccccac tcccaccctc aaccgcggcc cctgtaggcc aaggcgccag gcaggacgac
     2401 agcagcagca gcgcatctcc ttcagtccag ggagctccca gggaagtggt cgacccctcc
     2461 ggtggctggg ccactctgct agagtccatc cgccaagctg ggggcatcgg caaggccaag
     2521 ctgcgcagca tgaaggagcg aaagctggag aagcagcagc agaaggagca ggagcaagtg
     2581 agagccacga gccaaggtgg gcacttgatg tcggatctct tcaacaagct ggtcatgagg
     2641 cgcaagggca tctctgggaa aggacctggg gctggtgagg ggcccggagg agcctttgtc
     2701 cgcgtgtcag actccatccc tcctttgccg ccaccgcagc agccacaggc agaggaggac
     2761 gaggacgact gggaatcgta gggatccacc ggatctagat aactgatcat aatcagccat
     2821 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     2881 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     2941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     3001 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     3061 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3121 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3181 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3241 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3301 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3361 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3421 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3481 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3601 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     3661 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3721 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3781 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3841 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3901 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3961 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     4021 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     4081 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4141 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4201 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4261 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4321 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4381 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4441 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4501 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4561 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4621 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4681 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4741 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4801 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4861 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4921 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4981 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     5041 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     5101 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     5161 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     5221 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5281 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5341 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5401 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5461 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5521 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5581 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5641 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5701 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5761 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5821 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5881 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5941 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6001 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6061 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     6121 cat
//
