LOCUS       V033323                 6278 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033323
VERSION     V033323
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1363..2841
                     /label="VIPAS39(NM_001193314)"
                     /note="VIPAS39(NM_001193314)"
                     /gene="VIPAS39"
     CDS             2848..2874
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2878..2904
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2908..2934
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3066..3187
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3194..3649)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3676..3780
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3782..4139
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4174..4968
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5200..5247
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5576..6164
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagg aggaggggga agcgaagctt ccatgaatcg gacaaagggt
     1381 gatgaggagg agtattggaa cagctccaag ttcaaggctt ttacctttga cgatgaagac
     1441 gatgagcttt cacagttaaa ggagtccaag cgggcggtga acagcctccg agacttcgtg
     1501 gatgatgatg acgatgatga cctggagcga gtcagctgga gtggggaacc tgtgggaagt
     1561 atctcatggt ccatcagaga gactgctggt aatagcggct caacccacga ggggcgtgaa
     1621 cagctaaaga gccgaaacag cttctcctcc tatgcacaac tacccaagcc tacttctacc
     1681 tactccctga gcagcttttt tagaggtaga actagacctg gaagtttcca gtccctttct
     1741 gatgctctgt cagacacacc tgccaaaagc tatgctccag agctggggag acccaaaggg
     1801 gagtataggg attacagcaa tgactggagc cccagtgata cagtgcgacg tctccggaag
     1861 ggcaaggttt gctcactaga gagattccgc tccttacagg acaaactaca actcctagaa
     1921 gaggcagtaa gcatgcatga tggaaacgtc attactgcag ttctgatttt cctgaagagg
     1981 acactgagca aagagatcct cttccgagag ctggaggtgc gacaggttgc cctgagacat
     2041 cttattcact tccttaagga aataggggat caaaagttgc ttttagacct cttcaggttc
     2101 ctagatagaa cagaagagct tgcgctatct cattatcgag agcatttgaa cattcaggac
     2161 cctgacaaac gaaaagaatt tcttaaaacc tgtgttggtt tgccattttc agcagaagat
     2221 tccgcacaca tacaggacca ttacacgctc ctggaacgtc agatcattat tgaggcaaat
     2281 gatcgccatc tagaatcagc aggacagact gagatcttcc gaaagcaccc ccgcaaagcc
     2341 tccatcctca acatgccact agtgacaaca cttttctact cctgcttcta tcactacaca
     2401 gaggctgagg ggacattcag cagtcccgtc aacctgaaga agacatttaa gatcccagat
     2461 aaacagtatg tgctgacagc cctggctgct cgtgccaagc ttcgagcctg gaatgatgta
     2521 gatgccctat tcaccacaaa gaactggctg ggctatacca agaagagagc acccattggc
     2581 ttccatcggg ttgtcgaaat tttgcacaag aacaatgccc ctgtgcagat attacaggag
     2641 tatgtcaatc tggtggaaga tgtggacacg aagttgaact tagccactaa gttcaagtgc
     2701 catgatgtcg tcattgatac ctaccgggac ctgaaggatc gtcaacagtt gctagcatac
     2761 aggagtaagg tagataaagg atcagcagag gaggagaaga ttgatgctct tctcagcagc
     2821 tcgcaaattc gatggaagaa tggatcctac ccatacgacg tacctgatta tgcaacttat
     2881 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcatgagat
     2941 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     3001 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     3061 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3181 tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3241 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3301 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3361 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3421 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3481 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3541 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3601 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3661 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3721 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3781 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3901 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3961 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4021 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4081 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4141 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4201 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4261 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4321 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4381 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4441 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4501 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4561 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4621 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4681 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4741 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4801 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4861 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4921 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4981 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5041 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5101 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5161 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5221 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5281 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5341 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5401 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5701 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     5761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6241 cccctgattc tgtggataac cgtattaccg ccatgcat
//
