LOCUS       V037773                 8079 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V037773
VERSION     V037773
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced CFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNG
                     IKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="ECFP"
     CDS             1381..4734
                     /label="USP8(NM_005154)"
                     /note="USP8(NM_005154)"
                     /gene="USP8"
     polyA_signal    4867..4988
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4995..5450)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5477..5581
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5583..5940
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5975..6769
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7001..7048
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7377..7965
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagag cggcggaggg agtggaggag ggggaagacc
     1381 atgcctgctg tggcttcagt tcctaaagaa ctctacctca gttcttcact aaaagacctt
     1441 aataagaaga cagaagttaa accagagaaa ataagcacta agagttatgt gcacagtgcc
     1501 ctgaagatct ttaagacagc agaagaatgc agattagatc gtgatgagga aagggcctat
     1561 gtactatata tgaaatacgt gactgtttat aatcttatca aaaaaagacc tgatttcaag
     1621 caacagcagg attatttcca ttcaatactt ggacctggaa acatcaaaaa agctgtcgaa
     1681 gaagctgaaa gactctctga aagccttaaa ttaagatatg aagaagctga agtccggaaa
     1741 aaacttgagg aaaaagacag gcaggaggaa gcacagcggc tacaacaaaa aaggcaggaa
     1801 acaggaagag aggatggtgg cacattggct aaaggctctt tggagaatgt tttggattcc
     1861 aaagacaaaa cccaaaagag caatggtgaa aagaatgaaa aatgtgagac caaagagaaa
     1921 ggagcaatca cagcaaagga actatacaca atgatgacgg ataaaaacat cagcttgatt
     1981 ataatggatg ctcgaagaat gcaggattat caggattcct gtattttaca ttctctcagt
     2041 gttcctgaag aagccatcag tccaggagtc actgctagtt ggattgaagc acacctgcca
     2101 gatgattcta aagacacatg gaagaagagg gggaatgtgg agtatgtggt acttcttgac
     2161 tggtttagtt ctgccaaaga tttacagatt ggaacaactc tccggagtct gaaagatgca
     2221 cttttcaagt gggaaagtaa aactgtcctg cgcaatgagc ctttggtttt agagggaggc
     2281 tatgaaaact ggctcctttg ttatccccag tatacaacaa atgctaaggt cactccaccc
     2341 ccacgacgcc agaatgaaga ggtgtctatc tcattggatt ttacttatcc ctcattggaa
     2401 gaatcaattc cttctaaacc tgctgcccag acgccacctg catctataga agtagatgaa
     2461 aatatagaat tgataagtgg tcaaaatgag agaatgggac cactgaatat atcaactcca
     2521 gttgaaccag ttgctgcttc taaatctgat gtttcaccca taattcagcc agtgcctagt
     2581 ataaagaatg ttccacagat tgatcgtact aaaaaaccag cagtcaaatt gcctgaagag
     2641 catagaataa aatctgaaag tacaaaccat gagcaacaat ctcctcagag tggaaaagtt
     2701 attcctgatc gttccaccaa gccagtagtt ttttctccaa ctctcatgtt aacagatgaa
     2761 gaaaaggctc gtattcatgc agaaactgct cttctaatgg aaaaaaacaa acaagaaaaa
     2821 gaacttcggg aaaggcagca agaggaacag aaagagaaac tgaggaagga agaacaagaa
     2881 caaaaagcca aaaagaaaca agaagctgaa gaaaatgaaa ttacagagaa gcaacaaaaa
     2941 gcaaaagaag aaatggagaa gaaagaaagt gaacaggcca agaaagaaga taaagaaacc
     3001 tcagcaaaga ggggcaaaga aataacagga gtaaaaagac aaagtaaaag tgaacatgaa
     3061 acttctgatg ccaagaaatc tgtagaagat agggggaaaa ggtgtccaac cccagaaata
     3121 cagaaaaagt caacaggaga tgtgccccat acatctgtga caggggattc aggttcaggc
     3181 aagccattta agattaaagg acaaccagaa agtggaattc taaggacagg aacttttaga
     3241 gaggatacag acgataccga aagaaataaa gctcaacgag aacctttgac aagagcacga
     3301 agtgaagaaa tggggaggat cgtaccagga ctgccttcag gctgggccaa gtttcttgac
     3361 ccaatcactg gaacctttcg ttattatcat tcacccacca acactgttca tatgtaccca
     3421 ccggaaatgg ctccttcatc tgcacctcct tccacccctc caactcataa agccaagcca
     3481 cagattcctg ctgagcggga tagggaacct tccaaactga agcgctccta ctcctcccca
     3541 gatataaccc aggctattca agaggaagag aagaggaagc caacagtaac tccaacagtt
     3601 aatcgggaaa acaagccaac atgttatcct aaagctgaga tctcaaggct ttctgcttct
     3661 cagattcgga acctcaatcc tgtttttgga ggttctggac cagctcttac tggacttcgt
     3721 aacttaggaa atacttgtta tatgaactca atattgcagt gcctatgtaa cgctccacat
     3781 ttggctgatt atttcaaccg aaactgttat caggatgata ttaacaggtc aaatttgttg
     3841 gggcataaag gtgaagtggc agaagaattt ggtataatca tgaaagccct gtggacagga
     3901 cagtatagat atatcagtcc aaaggacttt aaaatcacca ttgggaagat caatgaccag
     3961 tttgcaggat acagtcagca agattcacaa gaattgcttc tgttcctaat ggatggtctc
     4021 catgaagatc taaataaagc tgataatcgg aagagatata aagaagaaaa taatgatcat
     4081 ctcgatgact ttaaagctgc agaacatgcc tggcagaaac acaagcagct caatgagtct
     4141 attattgttg cactttttca gggtcaattc aaatctacag tacagtgcct cacatgtcac
     4201 aaaaagtcta ggacatttga ggccttcatg tatttgtctc taccactagc atccacaagt
     4261 aaatgtacat tacaggattg ccttagatta ttttccaaag aagaaaaact cacagataac
     4321 aacagatttt actgcagtca ttgcagagct cgacgggatt ctctaaaaaa gatagaaatc
     4381 tggaagttac cacctgtgct tttagtgcat ctgaaacgtt tttcctacga tggcaggtgg
     4441 aaacaaaaat tacagacatc tgtggacttc ccgttagaaa atcttgactt gtcacagtat
     4501 gttattggtc caaagaacaa tttgaagaaa tataatttgt tttctgtttc aaatcactac
     4561 ggtgggctgg atggaggcca ctacacagcc tattgtaaaa atgcagcaag acaacggtgg
     4621 tttaagtttg atgatcatga agtttctgat atctccgttt cttctgtgaa atcttcagca
     4681 gcttatatcc tcttttatac ttcattggga ccacgagtaa ctgatgtagc cacataagga
     4741 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     4801 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4861 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4981 gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     5041 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5101 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5161 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5221 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5281 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5341 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5401 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     5461 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5521 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5581 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5701 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5761 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5821 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5881 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5941 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6001 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6061 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6121 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     6181 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6241 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6301 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6361 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6421 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6481 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6541 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6601 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6661 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6721 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6781 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6841 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6901 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     6961 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     7021 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     7081 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     7141 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     7201 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     7261 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7321 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7381 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7441 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7501 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7561 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7621 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7681 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7741 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7801 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7861 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7921 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7981 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8041 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
