LOCUS       V042634                 7100 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V042634
VERSION     V042634
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2997
                     /label="Trpv3(NM_145099)"
                     /note="Trpv3(NM_145099)"
                     /gene="Trpv3"
     CDS             3046..3765
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3888..4009
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4016..4471)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4498..4602
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4604..4961
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4996..5790
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6022..6069
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6398..6986
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgaat gcccactcca aggagatggc gcccctcatg
      661 ggcaaaagaa ccacggcacc tggcgggaac cctgttgtac tgacagagaa gaggccagca
      721 gatctcaccc ccaccaagaa gagtgcacac ttcttcctgg agatagaagg atttgagccc
      781 aaccccacgg tcaccaagac ctctccaccc atcttctcca agccgatgga ctccaacatc
      841 cggcagtgcc tctctggcaa ctgtgatgac atggactctc cccagtctcc tcaggatgat
      901 gtgacagaga ccccatccaa tcccaacagt ccgagcgcaa acctggccaa ggaagaacag
      961 aggcagaaga agaagcgact gaagaagcgc atcttcgcgg ctgtgtccga gggctgcgtg
     1021 gaggagctgc gggaactcct acaggatctg caggacctct gcaggaggcg ccgcggcctg
     1081 gatgtgcctg acttcctcat gcacaagctg acagcctcag acaccgggaa gacctgcctg
     1141 atgaaggctt tgctcaacat caatcccaac accaaagaga tcgtgcggat tctgcttgcc
     1201 ttcgctgagg agaacgacat cctggacagg ttcatcaacg ctgagtacac ggaagaggcc
     1261 tatgaagggc agacagcgct gaacatcgcc atcgagcggc gccagggaga catcacagca
     1321 gtgcttatag cagcgggtgc tgacgtcaat gctcacgcca agggggtctt cttcaacccc
     1381 aaataccagc atgaaggctt ctattttggc gagacacccc tggctttggc agcgtgtact
     1441 aaccagcctg agattgtgca gctgctgatg gagaatgagc agacagacat cacttcccag
     1501 gattcccggg gaaacaacat cctgcacgcg ctggtgacag tggctgagga cttcaagact
     1561 cagaatgact tcgttaagcg catgtatgac atgatcctgc tgaggagtgg caactgggag
     1621 ctggagacca tgcgcaacaa cgatgggctc acaccactgc agctggctgc caagatgggc
     1681 aaggctgaga tcctgaagta catcctcagc cgcgagatca aggagaagcc tctccggagc
     1741 ttgtccagga agttcacgga ctgggcgtat gggcctgtgt catcctcact ctatgacctc
     1801 accaatgtag acacaacgac ggataactct gtgctggaaa tcatcgtcta caacaccaac
     1861 attgataacc gacatgagat gctgaccctg gagcctctgc atacgctgct acacacgaaa
     1921 tggaagaaat ttgccaagta catgttcttc ttgtccttct gcttctattt cttctacaac
     1981 atcaccctga cccttgtctc ttactaccgt cctcgggaag atgaggatct cccacacccc
     2041 ttggccctga cacacaaaat gagttggctt cagctcctag ggaggatgtt tgtcctcatc
     2101 tgggccacat gcatctctgt gaaagaaggc attgccattt tcctgctgag accctccgat
     2161 cttcagtcca tcctgtcaga tgcctggttt cactttgtct tttttgtcca agctgtactt
     2221 gtgatactgt ctgtattctt gtacttgttt gcctacaaag aatacctcgc ctgcctcgtg
     2281 ctggccatgg ccctgggctg ggcgaacatg ctctactaca cgagaggctt ccagtctatg
     2341 ggcatgtaca gcgtcatgat ccagaaggtc attttgcatg atgtcctcaa gttcttgttt
     2401 gtttacatcc tgttcttact tggatttgga gtagcgctgg cctcactgat tgagaagtgc
     2461 tccaaggaca aaaaggactg cagttcctat ggcagcttca gcgacgcggt gctggagctc
     2521 ttcaagctca ccataggcct gggcgacctg aacatccagc agaactccac ctaccccatc
     2581 ctctttctct tcctactcat cacctatgtc atcctcacct tcgtcctcct cctcaacatg
     2641 ctcattgccc tgatggggga gacggtggag aacgtctcca aagaaagtga gcggatctgg
     2701 cgcttgcaga gagccaggac catcttggag tttgagaaaa tgttaccaga atggctgaga
     2761 agcagattcc gcatgggcga gctgtgcaaa gtagcagatg aggacttccg gctgtgtctg
     2821 cggatcaacg aggtgaagtg gacggaatgg aaaacacacg tgtccttcct taatgaagac
     2881 ccgggaccca taagacggac agcagattta aacaagattc aagattcttc caggagcaat
     2941 agcaaaacca ccctctatgc gtttgatgaa ttagatgaat tcccagaaac gtcggtgggt
     3001 ggaggcggat caggaggcgg tggatctgat ccaccggtcg ccaccatggt gagcaagggc
     3061 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3121 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3181 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3241 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3301 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3361 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3421 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3481 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3541 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3601 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3661 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3721 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     3781 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     3841 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     3901 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3961 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     4021 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     4081 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     4141 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     4201 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     4261 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     4321 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     4381 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     4441 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     4501 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4561 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     4621 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4681 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4741 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4801 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4861 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4921 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     4981 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5041 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5101 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5161 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5221 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5281 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     5341 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     5401 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     5461 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     5521 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5581 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5641 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5701 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5761 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5821 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     5881 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5941 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     6001 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     6061 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     6121 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     6181 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     6241 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6361 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6421 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6481 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6541 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6601 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6661 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6721 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6781 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6841 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6901 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6961 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7021 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7081 accgtattac cgccatgcat
//
