LOCUS V018724 5186 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018724 VERSION V018724 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 625..1110 /codon_start=1 /note="This allele of Thy1 is also known as CD90.2." /product="mouse GPI-anchored cell surface glycoprotein of the immunoglobulin superfamily" /transl_table=1 /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF CELQVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL" /label="Thy1.2" CDS 1132..1851 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 1974..2095 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2102..2557) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2584..2688 /gene="bla" /label="AmpR promoter" promoter 2690..3047 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3082..3876 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4108..4155 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4484..5072 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatgaac ccagccatca gcgtcgctct cctgctctca 661 gtcttgcagg tgtcccgagg gcagaaggtg accagcctga cagcctgcct ggtgaaccaa 721 aaccttcgcc tggactgccg ccatgagaat aacaccaagg ataactccat ccagcatgag 781 ttcagcctga cccgagagaa gaggaagcac gtgctctcag gcacccttgg gatacccgag 841 cacacgtacc gctcccgcgt caccctctcc aaccagccct atatcaaggt ccttacccta 901 gccaacttca ccaccaagga tgagggcgac tacttttgtg agcttcaagt ctcgggcgcg 961 aatcccatga gctccaataa aagtatcagt gtgtatagag acaagctggt caagtgtggc 1021 ggcataagcc tgctggttca gaacacatcc tggatgctgc tgctgctgct ttccctctcc 1081 ctcctccaag ccctggactt catttctctg ccggatccac cggtcgccac catggtgagc 1141 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 1201 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 1261 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 1321 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 1381 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 1441 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 1501 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 1561 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 1621 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 1681 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 1741 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 1801 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc 1861 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 1921 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 1981 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 2041 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 2101 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 2161 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 2221 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 2281 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 2341 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 2401 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 2461 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 2521 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 2581 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 2641 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 2701 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 2761 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 2821 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 2881 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 2941 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 3001 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 3061 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3121 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3181 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3241 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 3301 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 3361 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 3421 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 3481 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 3541 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 3601 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 3661 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 3721 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 3781 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 3841 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 3901 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 3961 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 4021 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 4081 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 4141 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 4201 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 4261 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 4321 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 4381 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 4441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 4501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 4561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 4621 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 4681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 4741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 4801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 4861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 4921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 4981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 5101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 5161 tggataaccg tattaccgcc atgcat //