LOCUS       V046098                 6158 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V046098
VERSION     V046098
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2049
                     /label="Tfeb(NM_001161723)"
                     /note="Tfeb(NM_001161723)"
                     /gene="Tfeb"
     CDS             2104..2823
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    2946..3067
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3074..3529)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3556..3660
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3662..4019
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4054..4848
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5080..5127
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5456..6044
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggcg tcacgcatcg ggctgcgcat gcagctcatg
      661 cgggagcagg cccagcagga ggagcagcga gagcgcatgc agcagcaggc tgtcatgcat
      721 tatatgcaac agcagcagca gcagcagcag cagctgggtg ggccccccac cccagccatc
      781 aacacccctg tccacttcca gtcgcccccg cctgtgcccg gggaggtgct gaaggtgcag
      841 tcctacctgg agaaccccac ctcctaccac ctgcaacagt cccagcatca gaaggttcgg
      901 gagtatctgt ctgagaccta tgggaacaag tttgctgccc acgtgagccc agcccaaggt
      961 tccccgaagc ctgccccagc agcatcccca ggggtgcggg ctggacacgt actgtccacc
     1021 tcggccggca acagtgctcc caacagtccc atggccatgc tacatatcag ctccaacccc
     1081 gagaaagagt ttgatgatgt cattgacaac attatgcgcc tggacagcgt gctgggctac
     1141 atcaaccctg agatgcagat gcctaacacg ctgcccctgt ctagcagcca cctgaacgtg
     1201 tacagcggtg acccccaggt cacagcctcc atggtgggtg tcaccagcag ctcctgccct
     1261 gccgacctga ctcagaagcg agagctaaca gatgctgaga gcagagccct ggccaaggag
     1321 cggcagaaga aagacaatca caacctaatt gagagaagac gcaggttcaa catcaatgac
     1381 cggatcaagg agctgggaat gctgatcccc aaggccaacg acctggacgt gcgctggaac
     1441 aaaggcacca tcctcaaggc ctctgtggat tacatccgga ggatgcagaa ggacctgcag
     1501 aagtcccggg agctggagaa ccactcccgg cgcctggaga tgactaacaa gcagctctgg
     1561 ctccgcatcc aggagctgga gatgcaggca cgcgtgcacg gcctccccac cacctcgccg
     1621 tcgggtgtga atatggccga gctggcccag caggtggtga agcaagagtt gcccagtgag
     1681 gatggcccag gggaggcgct gatgctgggg cctgaggtcc ctgagcctga gcaaatgccg
     1741 gctcttcctc cccaggctcc gctgccctcg gccgcccagc cacagtctcc gttccatcac
     1801 ctggacttca gccatggcct gagctttggg ggtgggggcg acgaggggcc cacaggttac
     1861 cccgataccc tggggacaga gcacggctcc ccattcccca acctgtccaa gaaggatctg
     1921 gacttaatgc tcctagatga ctccctgctc cccctggcct ctgaccccct cttttctacc
     1981 atgtctcctg aggcctccaa ggccagcagc cgccggagca gcttcagcat ggaggagggt
     2041 gatgttctga ccggtggcgg tggaggaagc ggcggaggtg gatctgatcc accggtcgcc
     2101 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     2161 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     2221 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     2281 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     2341 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     2401 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     2461 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     2521 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     2581 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     2641 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     2701 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     2761 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     2821 taaagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     2881 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     2941 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3001 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3061 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     3121 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3181 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3241 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3301 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3361 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3421 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3481 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     3541 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3601 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3661 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3721 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3841 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3901 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3961 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     4021 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4081 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4141 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4201 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     4261 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4321 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4381 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4441 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4501 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4561 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     4621 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4681 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4741 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     4801 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     4861 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4921 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4981 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     5041 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     5101 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     5161 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     5221 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     5281 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     5341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     5401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     5461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5581 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6121 cccctgattc tgtggataac cgtattaccg ccatgcat
//
