LOCUS       V020091                 7042 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020091
VERSION     V020091
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1375..3666
                     /label="TRPV2(NM_016113)"
                     /note="TRPV2(NM_016113)"
                     /gene="TRPV2"
     polyA_signal    3830..3951
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3958..4413)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4440..4544
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4546..4903
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4938..5732
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5964..6011
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6340..6928
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcag gaggaggggg aagcatgacc
     1381 tcaccctcca gctctccagt tttcaggttg gagacattag atggaggcca agaagatggc
     1441 tctgaggcgg acagaggaaa gctggatttt gggagcgggc tgcctcccat ggagtcacag
     1501 ttccagggcg aggaccggaa attcgcccct cagataagag tcaacctcaa ctaccgaaag
     1561 ggaacaggtg ccagtcagcc ggatccaaac cgatttgacc gagatcggct cttcaatgcg
     1621 gtctcccggg gtgtccccga ggatctggct ggacttccag agtacctgag caagaccagc
     1681 aagtacctca ccgactcgga atacacagag ggctccacag gtaagacgtg cctgatgaag
     1741 gctgtgctga accttaagga cggagtcaat gcctgcattc tgccactgct gcagatcgac
     1801 agggactctg gcaatcctca gcccctggta aatgcccagt gcacagatga ctattaccga
     1861 ggccacagcg ctctgcacat cgccattgag aagaggagtc tgcagtgtgt gaagctcctg
     1921 gtggagaatg gggccaatgt gcatgcccgg gcctgcggcc gcttcttcca gaagggccaa
     1981 gggacttgct tttatttcgg tgagctaccc ctctctttgg ccgcttgcac caagcagtgg
     2041 gatgtggtaa gctacctcct ggagaaccca caccagcccg ccagcctgca ggccactgac
     2101 tcccagggca acacagtcct gcatgcccta gtgatgatct cggacaactc agctgagaac
     2161 attgcactgg tgaccagcat gtatgatggg ctcctccaag ctggggcccg cctctgccct
     2221 accgtgcagc ttgaggacat ccgcaacctg caggatctca cgcctctgaa gctggccgcc
     2281 aaggagggca agatcgagat tttcaggcac atcctgcagc gggagttttc aggactgagc
     2341 cacctttccc gaaagttcac cgagtggtgc tatgggcctg tccgggtgtc gctgtatgac
     2401 ctggcttctg tggacagctg tgaggagaac tcagtgctgg agatcattgc ctttcattgc
     2461 aagagcccgc accgacaccg aatggtcgtt ttggagcccc tgaacaaact gctgcaggcg
     2521 aaatgggatc tgctcatccc caagttcttc ttaaacttcc tgtgtaatct gatctacatg
     2581 ttcatcttca ccgctgttgc ctaccatcag cctaccctga agaagcaggc cgcccctcac
     2641 ctgaaagcgg aggttggaaa ctccatgctg ctgacgggcc acatccttat cctgctaggg
     2701 gggatctacc tcctcgtggg ccagctgtgg tacttctggc ggcgccacgt gttcatctgg
     2761 atctcgttca tagacagcta ctttgaaatc ctcttcctgt tccaggccct gctcacagtg
     2821 gtgtcccagg tgctgtgttt cctggccatc gagtggtacc tgcccctgct tgtgtctgcg
     2881 ctggtgctgg gctggctgaa cctgctttac tatacacgtg gcttccagca cacaggcatc
     2941 tacagtgtca tgatccagaa ggtcatcctg cgggacctgc tgcgcttcct tctgatctac
     3001 ttagtcttcc ttttcggctt cgctgtagcc ctggtgagcc tgagccagga ggcttggcgc
     3061 cccgaagctc ctacaggccc caatgccaca gagtcagtgc agcccatgga gggacaggag
     3121 gacgagggca acggggccca gtacaggggt atcctggaag cctccttgga gctcttcaaa
     3181 ttcaccatcg gcatgggcga gctggccttc caggagcagc tgcacttccg cggcatggtg
     3241 ctgctgctgc tgctggccta cgtgctgctc acctacatcc tgctgctcaa catgctcatc
     3301 gccctcatga gcgagaccgt caacagtgtc gccactgaca gctggagcat ctggaagctg
     3361 cagaaagcca tctctgtcct ggagatggag aatggctatt ggtggtgcag gaagaagcag
     3421 cgggcaggtg tgatgctgac cgttggcact aagccagatg gcagccccga tgagcgctgg
     3481 tgcttcaggg tggaggaggt gaactgggct tcatgggagc agacgctgcc tacgctgtgt
     3541 gaggacccgt caggggcagg tgtccctcga actctcgaga accctgtcct ggcttcccct
     3601 cccaaggagg atgaggatgg tgcctctgag gaaaactatg tgcccgtcca gctcctccag
     3661 tccaactgag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
     3721 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     3781 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     3841 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3901 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa
     3961 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     4021 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     4081 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     4141 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     4201 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     4261 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     4321 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     4381 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     4441 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4501 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     4561 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4621 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4681 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4741 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4801 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4861 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     4921 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4981 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     5041 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     5101 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     5161 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     5221 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     5281 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     5341 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     5401 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5461 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5521 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5581 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5641 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5701 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     5761 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5821 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5881 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     5941 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     6001 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     6061 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     6121 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     6181 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6241 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6301 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6361 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6421 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6481 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6541 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6601 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6661 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6721 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6781 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6841 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6901 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6961 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7021 taaccgtatt accgccatgc at
//
