LOCUS       V045974                 6630 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045974
VERSION     V045974
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="enhanced blue variant of GFP"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EBFP"
     CDS             1396..3285
                     /label="TRIM25(NM_005082)"
                     /note="TRIM25(NM_005082)"
                     /gene="TRIM25"
     polyA_signal    3418..3539
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3546..4001)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4028..4132
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4134..4491
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4526..5320
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5552..5599
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5928..6516
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc cacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaacttc aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcgggtgg aggcggatca
     1381 ggaggcggtg gatctatggc agagctgtgc cccctggccg aggagctgtc gtgctccatc
     1441 tgcctggagc ccttcaagga gccggtcacc actccgtgcg gccacaactt ctgcgggtcg
     1501 tgcctgaatg agacgtgggc agtccagggc tcgccatacc tgtgcccgca gtgccgcgcc
     1561 gtctaccagg cgcgaccgca gctgcacaag aacacggtgc tgtgcaacgt ggtggagcag
     1621 ttcctgcagg ccgacctggc ccgggagcca cccgccgacg tctggacgcc gcccgcccgc
     1681 gcctctgcac ccagcccgaa tgcccaggtg gcctgcgacc actgcctgaa ggaggccgcc
     1741 gtgaagacgt gcttggtgtg catggcctcc ttctgtcagg agcacctgca gccgcacttc
     1801 gacagccccg ccttccagga ccacccgctg cagccgcccg ttcgcgacct gttgcgccgc
     1861 aaatgttccc agcacaatcg gctgcgggaa tttttctgcc ccgagcacag cgagtgcatc
     1921 tgccacatct gcctggtgga gcataagacc tgctctcccg cgtccctgag ccaggccagc
     1981 gccgacctgg aggccaccct gaggcacaaa ctaactgtca tgtacagtca gatcaacggg
     2041 gcgtcgagag cactggatga tgtgagaaac aggcagcagg atgtgcggat gactgcaaac
     2101 agaaaggtgg agcagctaca acaagaatac acggaaatga aggctctctt ggacgcctca
     2161 gagaccacct cgacaaggaa gataaaggaa gaggagaaga gggtcaacag caagtttgac
     2221 accatttatc agattctcct caagaagaag agtgagatcc agaccttgaa ggaggagatt
     2281 gaacagagcc tgaccaagag ggatgagttc gagtttctgg agaaagcatc aaaactgcga
     2341 ggaatctcaa caaagccagt ctacatcccc gaggtggaac tgaaccacaa gctgataaaa
     2401 ggcatccacc agagcaccat agacctcaaa aacgagctga agcagtgcat cgggcggctc
     2461 caggagccca cccccagttc aggtgaccct ggagagcatg acccagcgtc cacacacaaa
     2521 tccacacgcc ctgtgaagaa ggtctccaaa gaggaaaaga aatccaagaa acctccccct
     2581 gtccctgcct tacccagcaa gcttcccacg tttggagccc cggaacagtt agtggattta
     2641 aaacaagctg gcttggaggc tgcagccaaa gccaccagct cacatccgaa ctcaacatct
     2701 ctcaaggcca aggtgctgga gaccttcctg gccaagtcca gacctgagct cctggagtat
     2761 tacattaaag tcatcctgga ctacaacacc gcccacaaca aagtggctct gtcagagtgc
     2821 tatacagtag cttctgtggc tgagatgcct cagaactacc ggccgcatcc ccagaggttc
     2881 acatactgct ctcaggtgct gggcctgcac tgctacaaga aggggatcca ctactgggag
     2941 gtggagctgc agaagaacaa cttctgtggg gtaggcatct gctacggaag catgaaccgg
     3001 cagggcccag aaagcaggct cggccgcaac agcgcctcct ggtgcgtgga gtggttcaac
     3061 accaagatct ctgcctggca caataacgtg gagaaaaccc tgccctccac caaggccacg
     3121 cgggtgggcg tgcttctcaa ctgtgaccac ggctttgtca tcttcttcgc tgttgccgac
     3181 aaggtccacc tgatgtataa gttcagggtg gactttactg aggctttgta cccggctttc
     3241 tgggtatttt ctgctggtgc cacactctcc atctgctccc ccaagtaggg atccaccgga
     3301 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     3361 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     3421 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3481 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     3541 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3601 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3661 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3721 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3781 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3841 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3901 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3961 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     4021 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4081 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     4141 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4201 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4261 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4321 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4381 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4441 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     4501 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4561 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4621 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4681 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     4741 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4801 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4861 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4921 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4981 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5041 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5101 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5161 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5221 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5281 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5341 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5401 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5461 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     5521 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     5581 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     5641 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     5701 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     5761 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5821 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5881 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     5941 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6001 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6061 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6121 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6181 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6241 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6301 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6361 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6421 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6481 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6541 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6601 tctgtggata accgtattac cgccatgcat
//
