LOCUS V041909 6537 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V041909 VERSION V041909 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1323 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of red fluorescent protein from Entacmaea quadricolor (Merzlyak et al., 2007)" /transl_table=1 /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV ARYCDLPSKLGHKLN" /label="TagRFP" CDS 1354..3138 /label="Snx9(NM_025664)" /note="Snx9(NM_025664)" /gene="Snx9" CDS 3139..3162 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 3325..3446 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3453..3908) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3935..4039 /gene="bla" /label="AmpR promoter" promoter 4041..4398 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4433..5227 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5459..5506 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5835..6423 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag 661 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc 721 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc 781 ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac 841 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga 901 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac 961 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg 1021 atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc 1081 ggcctggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca cctgatctgc 1141 aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc 1201 tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag 1261 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt 1321 aatggtggag gcggatcagg aggcggtgga tctatggcca ccaaggctcg ggtcatgtac 1381 gactttgctg ctgagcctgg aaacaatgag ctgaccgtta ctgaaggaga aatcatcaca 1441 gttacaaacc cgaatgttgg tggaggatgg ctggaaggaa agaacaacaa aggagagcag 1501 gggcttgttc ctactgacta cgtggaaatt ttaccaaacg atggaaaaga tccgttttct 1561 tgtgggaatt cagtagccga ccaagctttc ctcgattccc tgacagctag cacagctcaa 1621 accaactcat cttctgccaa cagcaataac caggttggtg gtggcaatga cccctggaca 1681 gcctggaatg ctcccaaacc tgggaactgg gacagctccg atgcctgggg ctccaggaca 1741 gacggaacta gcgctcagag gaacagcagc gccaacaact gggacacggg ctttggccac 1801 ccacaggcct accaaggacc agcaactggt gatgatgacg aatgggatga agactgggac 1861 gaccccaagt cctcctcacc ttacttcaag gattcagaac cagctgaagc agggggcatc 1921 cagcggggaa acagtcgtgc gggtgcctcc tccatgaagc tgccccttaa caaatttcct 1981 ggatttgcaa aacctggcat ggagcagtac ttgttggcca agcagctagc aaaacccaaa 2041 gagaaaatcg ccatcatcgt tggagattat ggccctatgt gggtttatcc tacctctacg 2101 tttgactgtg tggtagctga tccccggaaa ggttccaaaa tgtacggtct gaagagctac 2161 attgagtatc agctaacacc cactaacact aaccgatctg taaaccacag atataagcac 2221 tttgattggt tatatgagcg tctcctggtt aagtttgggt ctgccattcc gatcccttct 2281 cttccagaca agcaggtcac aggtcgcttt gaagaggaat ttatcaaaat gcggatggag 2341 cggctccagg cctggatgac caggatgtgc cggcacccgg tggtgtcaga aagtgaagtt 2401 ttccagcagt tcctgaattt ccgagatgag aaggaatgga agactggaaa gaggaaggct 2461 gagaaagacg aactggtggg agtgatgata ttttccacca tggaaccaga ggctcctgac 2521 ttggatttga tagaaataga acagaagtgt gacgctgtgg ggaagttcac caaggccatg 2581 gatgacggcg tgaaggagct gctgacggtg gggcaggagc actggaagcg ctgcacaggg 2641 cccttaccca aggagtatca gaagataggg aaggctctgc agagcttagc ggcagtgttc 2701 agctccagcg gttatcaagg agagaccgac cttaacgatg caatcacaga agctgggaag 2761 acgtatgagg agatcgccag ccttgtggca gagcagccaa agaaggacct ccacttcctg 2821 atggagtgca accatgagta caaaggcttt ctcggctgct tcccggacat cattggtgcc 2881 cacaagggag caatagaaaa agtgaaagaa agtgataagc tggttgcaac tagtaaaatt 2941 actccccaag acaagcagac catggtgaag agagttggca ccatgtctta cgctctgcaa 3001 gctgagatga atcactttca cagtaaccgg atctatgatt acaacagtgt catccgcctc 3061 tacctggagc agcaagtgca gttctatgaa acaatcgcag aaaagctgag gcaggccctc 3121 agccgcttcc cggttatgga ctacaaggac gacgatgaca agtagaattc tgcagtcgac 3181 ggtaccgcgg gcccgggatc caccggatct agataactga tcataatcag ccataccaca 3241 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 3301 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 3361 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 3421 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa 3481 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3541 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3601 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3661 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3721 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 3781 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 3841 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 3901 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 3961 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 4021 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta 4081 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 4141 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 4201 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 4261 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 4321 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 4381 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga 4441 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4501 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4561 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 4621 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4681 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4741 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4801 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 4861 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 4921 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 4981 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5041 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5101 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5161 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5221 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 5281 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 5341 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct 5401 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg 5461 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt 5521 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc 5581 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca 5641 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt 5701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc 5761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5821 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5881 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5941 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 6001 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 6061 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 6121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 6181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 6241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 6301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 6361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 6421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6481 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat //