LOCUS       V025233                 6966 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025233
VERSION     V025233
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1378..3621
                     /label="Smurf2(NM_001107061)"
                     /note="Smurf2(NM_001107061)"
                     /gene="Smurf2"
     polyA_signal    3754..3875
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3882..4337)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4364..4468
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4470..4827
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4862..5656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5888..5935
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6264..6852
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg
     1381 tctaaccccg gaggccggag gaacgggccc gtcaagctgc gcctgacagt actctgtgca
     1441 aaaaacttgg tgaaaaagga ttttttccga ctccctgatc cgtttgctaa ggtggtagtt
     1501 gatggttctg ggcaatgcca ttctacagat actgtgaaga acacacttga tccaaagtgg
     1561 aatcagcatt atgacctgta tattgggaag tctgattccg tcacgatcag cgtgtggaat
     1621 cataagaaga tccataagaa gcaaggtgct ggatttcttg gttgtgttcg tcttctttcc
     1681 aatgccatca atcgcctcaa agacactggc tatcagaggt tggatttatg caaactgggg
     1741 ccaaatgaca atgatacagt tcgaggacaa attgtagtaa gtcttcaatc cagagaccga
     1801 ataggcacgg gaggacaagt tgtggactgc agtcgtttgt ttgataatga tttaccagac
     1861 ggttgggaag aaaggcgaac tgcctctgga agaatccagt atctaaacca catcacaaga
     1921 actacacagt gggaacgccc aacaagaccg gcgtcagaat attctagtcc tggcaggcct
     1981 cttagctgct ttgtggatga gaatactcca attactggaa caaatggtgc aacatgtgga
     2041 cagtcttcag atcccagact agcagagagg agagtccggt ctcagcgaca tagaaattac
     2101 atgagcagga cacacttaca cactcctcca gacctacccg aaggctatga acagaggaca
     2161 acacaacagg gtcaggtata tttcttacat actcagactg gcgtgagcac gtggcatgat
     2221 ccacgagtgc ctagggatct tagcaacatc aattgtgaag agctcggtcc tctgcctcct
     2281 gggtgggaga tccgcaatac cgcgacaggc agagtttatt tcgtggacca taacaacaga
     2341 acaacacagt ttacagatcc tcgtctctct gctaacttgc atttagtttt aaatcgtcag
     2401 aaccagttga aagaccaaca gcaacagcaa gtggtgtcgt tgtgtcccga tgacactgag
     2461 tgtctgacag tgccaaggta caagcgagac ttggttcaaa aactaaagat cttgcggcaa
     2521 gaactttccc aacagcagcc tcaagctggc cactgccgca ttgaggtgtc aagggaagag
     2581 atttttgagg aatcatatcg acaagtcatg aaaatgagac caaaggatct atggaagcga
     2641 ttaatgataa aatttcgagg agaagaaggc cttgactatg gaggagttgc cagggaatgg
     2701 ttgtatctcc tgtcgcatga aatgttgaat ccttactacg gtctcttcca gtattcaaga
     2761 gatgatatct acacgttaca gatcaatccc gattctgcag ttaatccgga gcatttgtcc
     2821 tatttccact ttgttggacg aataatggga atggctgtat tccatggaca ttatattgat
     2881 ggtggtttca cattaccttt ttataaacag ttgcttggga agtcaattac tttggacgac
     2941 atggagctag ttgatccaga ccttcataat agtttggtgt ggatacttga aaatgatatc
     3001 acaggtgttc tggaccatac cttctgtgtt gaacataacg catatggtga aattattcag
     3061 catgaactca aaccaaatgg caaaagtatt cctgttactg aagaaaataa aaaagaatat
     3121 gtcaggcttt acgtaaactg gagattcctc cgaggcattg aagctcagtt cctggcacta
     3181 cagaagggat ttaatgaagt cattccacag catctgctga agacatttga tgagaaggag
     3241 ctggagctca ttatctgtgg gcttggcaaa attgacgtga gcgactggaa ggccaacacc
     3301 cggttaaagc actgcacccc agacagcaac gtcgtcaagt ggttttggaa agctgtggag
     3361 ctttttgatg aggagagacg agcacggctg ctccagtttg tgacagggtc ctctcgagtg
     3421 cctctgcagg gcttcaaagc actccaaggt gccgcgggcc cacggctctt taccatacac
     3481 cagattgatg cctgcacgaa caacctgcca aaagcccaca cttgtttcaa tcgaatagac
     3541 attccgccct atgagagcta tgagaagctc tacgaaaagc tgctaacagc catcgaggag
     3601 acctgtggct ttgctgtgga gtgaggatcc accggatcta gataactgat cataatcagc
     3661 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3721 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3841 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     3901 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3961 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     4021 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4081 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4141 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4201 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4261 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4321 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4381 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4441 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4501 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4561 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4621 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4681 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4741 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4801 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     4861 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     4921 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     4981 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     5041 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     5101 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     5161 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     5221 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     5281 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     5341 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     5401 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     5461 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     5521 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     5581 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     5641 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     5701 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     5761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     5821 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     5881 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     5941 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     6001 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     6061 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     6121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     6421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     6961 atgcat
//
