LOCUS       V018773                 6378 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018773
VERSION     V018773
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2283
                     /label="Slc22a4(NM_022270)"
                     /note="Slc22a4(NM_022270)"
                     /gene="Slc22a4"
     CDS             2335..3045
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK*"
                     /label="mCherry"
     polyA_signal    3166..3287
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3294..3749)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3776..3880
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3882..4239
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4274..5068
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5300..5347
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5676..6264
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgagg gactatgacg aggtgatagc cttcctgggc
      661 gattgggggc ccttccagcg ccttatcttc ttcctgctca gcgccagcat catccccaat
      721 ggcttcaatg gtatgtcagt cgtgttcctg gcggggaccc cggagcatcg ttgccttgtg
      781 cctcacaccg tgaacttgag cagcgcgtgg cgcaaccaca gcatcccctt ggaaacgaag
      841 gacggacgac aggtgcctca aagctgccgc cgctaccgac tggccaccat cgccaacttc
      901 tctgcgctgg ggctggagcc cggactggat gtagatctgg agcagctgga gcaagagagc
      961 tgcctggatg gctgggagta cagcaaggac gtcttcctgt ccaccatcgt gacagagtgg
     1021 aatctggtgt gtgaggatga ctggaagaca cccctcacca cctccctgtt cttcgtaggc
     1081 gttctctgtg gctccttcgt gtctgggcag ctgtcagaca ggttcggcag gaagaaagtc
     1141 ctctttgcca ccatggctgt gcagactgga ttcagcttcg tgcagatttt ctcaaccaac
     1201 tgggagatgt ttactgtgtt atttgccatc gtgggcatgg gccaaatctc caactatgtg
     1261 gtggccttca tactcgggac cgaaatcctg agcaagtcgg ttcgcatcct cttctccacg
     1321 ttgggagtct gtacattttt tgccatcggc tacatggtgc tgccgctgtt tgcatacttc
     1381 atccgagact ggaggatgct gctgctggca ctgacactgc ctgggctgtt ctgtgtcccc
     1441 ctgtggtggt ttattccaga gtctccccgg tggctgatat cccagaggag atttgaagag
     1501 gcagaacaga tcatccagaa agccgcaaag atgaatggca tcatggcacc agcagtgata
     1561 tttgatcctc tggagctaca ggagctaaac tccttgaagc agcagaaagt cttcattctg
     1621 gacctgttca agactcggaa cattgccaca ataacagtga tgtctgtgat gctgtggatg
     1681 ctaacctcag tgggttactt tgctctgtct ctcaatgttc ctaatttgca cggagatgtc
     1741 tacctgaact gcttcctctc tggcctgatt gaagttccag cttatttcac agcctggctg
     1801 ctacttcgaa ccctgcctcg gagatatatt atagctgggg tgctgttctg gggaggaggt
     1861 gtgcttctct tggtccaagt ggtacctgaa gattacaact ttgtgtccat tggcctggtg
     1921 atgctgggga aatttggggt cacctctgcc ttctccatgc tgtacgtctt cacagcggag
     1981 ctctacccaa ccctggtcag gaacatggct gtgggcatca cctccatggc ctcgagggtg
     2041 ggcagcatca ttgcccccta tttcgtgtac ctgggtgctt ataaccgact cctgccctac
     2101 atcctcatgg gcagtctgac tgtcctcatt ggaatcatca cacttttttt ccctgagagt
     2161 tttggagtga ctctaccaga gaacttggag cagatgcaga aagtcagagg gttcagatgt
     2221 gggaaaaaat caacagtctc catggacaga gaagaaaacc ccaaggttct aataactgca
     2281 ttcagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg
     2341 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     2401 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     2461 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     2521 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     2581 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     2641 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     2701 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     2761 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     2821 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     2881 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     2941 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     3001 gagggccgcc actccaccgg cggcatggac gagctgtaca agtagcggcc gcgactctag
     3061 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     3121 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     3181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     3301 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     3361 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     3421 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     3481 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3541 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3601 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3661 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3721 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     3781 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3841 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     3901 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3961 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4081 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4141 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4201 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     4261 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     4321 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     4381 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     4441 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     4501 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     4561 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     4621 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     4681 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     4741 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     4801 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     4861 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     4921 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     4981 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     5041 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     5101 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     5161 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     5221 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     5281 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     5341 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     5401 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     5461 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     5521 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5581 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5641 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5701 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5761 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5821 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5881 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5941 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6001 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6061 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6121 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6181 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6241 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6301 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6361 cgtattaccg ccatgcat
//
