LOCUS       V027778                 7105 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027778
VERSION     V027778
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             624..3002
                     /label="Sel1l(NM_177933)"
                     /note="Sel1l(NM_177933)"
                     /gene="Sel1l"
     CDS             3051..3770
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3893..4014
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(4021..4476)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4503..4607
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4609..4966
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5001..5795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6027..6074
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      6403..6991
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagcc accatgcagg tccgcgtcag actgttgttg ctgctctgcg
      661 cggtgctcct gggctcggcc gccgcgtcct cagatgagga aactaaccag gatgaatcct
      721 tagattccaa gggtgcttta cccacagatg ggtcggtgaa ggaccacacc actggcaaag
      781 tagttgctgg ccagagattt gttgattctg aggactcaga ggtggaatcc cttcttcagg
      841 acgaggaaga gagctccaag agccaggagg aagtcagtgt cacggaagac atcagctttt
      901 tagactctcc aaatccaagc agcaagacct acgaagaact aaagagagtg cggaagccag
      961 ttttgactgc cattgaaggt acggcgcacg gggagccctg ccacttccct ttccttttcc
     1021 tggataagga atatgatgag tgcacatcag acgggaggga agatggcaga ctgtggtgtg
     1081 ccacaaccta tgactacaag acagatgaga agtggggctt ctgcgaaacc gaagaagatg
     1141 ctgccaaaag gcgacagatg caggaagcag aagccatcta tcagagcggg atgaagatcc
     1201 tgaacggcag caccaggaag aaccaaaaga gagaagcata tcggtacctt cagaaggcag
     1261 caggcatgaa tcacaccaaa gccctggaga gagtgtccta tgctctcttg tttggtgatt
     1321 acctcacaca gaatatccag gcagctaaag agatgtttga gaaactgact gaggaagggt
     1381 ctcccaaagg acaaactggt cttggctttc tctacgcttc tggacttggt gttaattcaa
     1441 gtcaggcgaa ggctcttgtg tattacactt ttggagctct tggaggcaac ctgatagcgc
     1501 atatggtttt gggttaccgc tactgggctg gcatcggagt cctccagagt tgcgagtcgg
     1561 cactgaccca ttatcgtctt gttgccaatc atgtcgctag tgatatctcc ctaactggag
     1621 gctctgtagt ccagagaata cggctgcctg atgaagtgga aaacccaggg atgaacagtg
     1681 ggatgctgga agaagatctg attcagtatt accagttcct agctgagaag ggtgatgtcc
     1741 aagcacaggt tggtctggga cagctgcatc tgcatggagg gcgtggagta gaacagaatc
     1801 accagagagc gtttgactac ttcaacttag cagcaaatgc tggcaattca catgctatgg
     1861 ccttcctggg aaagatgtat tctgagggaa gtgacatcgt gcctcagagt aacgagacgg
     1921 cacttcacta cttcaagaaa gctgctgaca tgggcaaccc ggtgggacag agcggacttg
     1981 gaatggccta cctctatgga agaggagttc aagttaatta tgacctggca ctcaagtatt
     2041 tccagaaagc tgctgagcaa ggctgggtgg acgggcagct gcagcttggc tctatgtact
     2101 acaatggcat tggagtcaag agagattata aacaggcctt gaagtatttt aatttagctt
     2161 ctcagggagg ccatatcttg gctttctata acctagcaca gatgcacgcc agcggcacag
     2221 gggtgatgag atcctgccac actgcagtgg agttgtttaa gaacgtgtgt gagcgtggtc
     2281 gctggtcaga gaggctgatg accgcctaca acagctataa agacgacgac tacaacgccg
     2341 ctgtggtcca gtacctcctg ctggcggagc agggctacga ggtggcgcag agcaacgcag
     2401 ccttcatcct cgaccagaga gaagcaacca ttgtaggtga gaatgaaact taccccagag
     2461 ctttgctgca ttggaatagg gccgcctccc aaggttatac tgtggctaga attaaacttg
     2521 gagactacca tttctatgga tttggcactg atgtggatta cgagactgcg tttattcatt
     2581 accgcctggc ctcagagcaa cagcacagtg cccaagctat gtttaacctg gggtacatgc
     2641 atgagaaggg cctaggcatt aaacaggata tccacctggc aaaacgcttt tatgacatgg
     2701 cagccgaagc cagcccagat gcacaagtac ctgtattcct cgcactctgc aaattaggtg
     2761 tcgtctattt cttacagtac atacgggaag caaacattcg agatctattc acacaactgg
     2821 atatggacca gctcttggga cctgagtggg atctttacct catgaccatc attgcactgc
     2881 tgttgggtac agtcatagcc tacaggcagc gacagcacca ggacatacca gttcccaggc
     2941 ccccagggcc acggccagct cctccccagc aggaaggacc cccagagcag cagccaccac
     3001 agggtggagg tggatctgga ggtggaggtt cggatccacc ggtcgccacc atggtgagca
     3061 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3121 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3181 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3241 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     3301 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3361 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3421 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3481 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3541 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     3601 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     3661 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     3721 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccgcg
     3781 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3841 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3901 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3961 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     4021 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     4081 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     4141 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     4201 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     4261 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4321 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4381 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4441 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     4501 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4561 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     4621 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4801 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4861 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4921 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4981 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5041 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5101 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5161 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     5221 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5281 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5341 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5401 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5461 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5521 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     5581 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5641 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5701 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5761 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5821 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5881 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5941 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     6001 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     6061 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     6121 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     6181 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     6241 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6301 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6361 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6421 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6481 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6541 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6601 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6661 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6721 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6781 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6841 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6901 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6961 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7021 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7081 ggataaccgt attaccgcca tgcat
//
