LOCUS       V031885                 6162 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V031885
VERSION     V031885
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..2817
                     /label="SYT7(NM_001252065)"
                     /note="SYT7(NM_001252065)"
                     /gene="SYT7"
     polyA_signal    2950..3071
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3078..3533)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3560..3664
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3666..4023
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4058..4852
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5084..5131
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5460..6048
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatgtacc gggacccgga ggcggccagc ccaggggcgc cctcgcgcga cgtcctgctg
     1441 gtctctgcca tcatcaccgt cagccttagc gtcactgtcg tcctctgcgg cctctgccac
     1501 tggtgtcagc gcaaactggg caaacgctac aagaattcct tggagacggt gggcacgcca
     1561 gactcagggc gtgggcgcag tgagaagaag gctatcaatg gcaccctcct gtcgggcgcc
     1621 aaagtggccg ccgcggcggg gctggcggtg gagcgggaag gccggctggg ggagaagccg
     1681 gcaccggtgc cgccacccgg agaggacgcc ttgagaagcg gcggggctgc ccccagcgag
     1741 ccgggcagcg gtggcaaggc ggggagaggc cgctggcgga cggtgcagag ccacctggcc
     1801 gcagggaagc tcaacttgtc caagttgcct gcaggaggga aggcggtgaa cacagccccc
     1861 gtgccaggcc agacacccca cgatgagtcc gaccgccgga ccgagccacg ttcctccgtc
     1921 tcagacctcg tcaactccct caccagcgag atgctcatgc tctccccagg ctccgaggag
     1981 gatgaggccc acgagggttg cagccgagag aacctgggcc ggatccagtt cagtgtcggc
     2041 tacaacttcc aggagtccac gctcaccgtg aagatcatga aggcccagga gctgccggcc
     2101 aaggacttca gcggcaccag cgaccccttc gtcaagatct acctgctgcc cgacaagaag
     2161 cacaagctgg agaccaaggt gaagcggaag aacctgaacc cccactggaa cgagaccttc
     2221 ctctttgaag gttttcccta tgagaaggtg gtgcagagga tcctctacct ccaagtcctg
     2281 gactatgacc gcttcagccg caacgacccc attggggagg tgtccatccc ccttaacaag
     2341 gtggacctga cccagatgca gaccttctgg aaggatctga agccatgcag cgatgggagt
     2401 gggagccgag gggagctgct cttgtctctc tgctacaacc cctctgccaa ctccatcatc
     2461 gtgaacatca tcaaagcccg gaacctcaaa gccatggaca tcgggggcac atcagacccc
     2521 tacgtgaagg tatggctgat gtacaaggac aagcgggtgg agaagaagaa gacggtgacg
     2581 atgaagagga acctgaaccc catcttcaat gagtccttcg ccttcgatat ccccacggag
     2641 aagctgaggg agacgaccat catcatcact gtcatggaca aggacaagct cagccgcaat
     2701 gacgtcatcg gcaagatcta cctgtcctgg aagagcgggc caggggaggt gaagcactgg
     2761 aaggacatga ttgcccgtcc ccggcagccc gtggcccagt ggcaccagct gaaggcctga
     2821 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     2881 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     2941 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3001 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3061 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3121 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3181 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3241 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3301 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3361 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3421 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3481 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     3541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3661 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     3721 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     3781 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     3841 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     3901 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     3961 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4021 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4081 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4141 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     4201 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     4261 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     4321 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     4381 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4441 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4501 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4561 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     4621 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4681 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4741 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4801 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     4861 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     4921 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4981 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     5041 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5101 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     5161 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     5221 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     5281 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     5341 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5401 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5461 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5521 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5581 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5641 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5701 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5761 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5821 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5881 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5941 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6001 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6061 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6121 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
