LOCUS       V036391                 5628 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036391
VERSION     V036391
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1305
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             1342..2283
                     /label="SPP1(NM_001040058)"
                     /note="SPP1(NM_001040058)"
                     /gene="SPP1"
     polyA_signal    2416..2537
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2544..2999)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3026..3130
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3132..3489
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3524..4318
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4550..4597
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      4926..5514
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatgagcga gctgatcaag gagaacatgc acatgaagct gtacatggag
      661 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      721 ggcacccaga ccatgaagat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      781 atcctggcta ccagcttcat gtacggcagc aaagccttca tcaaccacac ccagggcatc
      841 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagaat caccacatac
      901 gaagacgggg gcgtgctgac cgctacccag gacaccagct tccagaacgg ctgcatcatc
      961 tacaacgtca agatcaacgg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     1021 acacgcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctgagaggc
     1081 cacagccaga tggccctgaa gctcgtgggc gggggctacc tgcactgctc cttcaagacc
     1141 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcttcca cttcgtggac
     1201 cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgagatg
     1261 gctgtggcca agtactgcga cctccctagc aaactggggc acagaggcgg cggagggagt
     1321 ggaggagggg gaagcgaatt catgagaatt gcagtgattt gcttttgcct cctaggcatc
     1381 acctgtgcca taccagttaa acaggctgat tctggaagtt ctgaggaaaa gcagctttac
     1441 aacaaatacc cagatgctgt ggccacatgg ctaaaccctg acccatctca gaagcagaat
     1501 ctcctagccc cacagaatgc tgtgtcctct gaagaaacca atgactttaa acaagagacc
     1561 cttccaagta agtccaacga aagccatgac cacatggatg atatggatga tgaagatgat
     1621 gatgaccatg tggacagcca ggactccatt gactcgaacg actctgatga tgtagatgac
     1681 actgatgatt ctcaccagtc tgatgagtct caccattctg atgaatctga tgaactggtc
     1741 actgattttc ccacggacct gccagcaacc gaagttttca ctccagttgt ccccacagta
     1801 gacacatatg atggccgagg tgatagtgtg gtttatggac tgaggtcaaa atctaagaag
     1861 tttcgcagac ctgacatcca gtaccctgat gctacagacg aggacatcac ctcacacatg
     1921 gaaagcgagg agttgaatgg tgcatacaag gccatccccg ttgcccagga cctgaacgcg
     1981 ccttctgatt gggacagccg tgggaaggac agttatgaaa cgagtcagct ggatgaccag
     2041 agtgctgaaa cccacagcca caagcagtcc agattatata agcggaaagc caatgatgag
     2101 agcaatgagc attccgatgt gattgatagt caggaacttt ccaaagtcag ccgtgaattc
     2161 cacagccatg aatttcacag ccatgaagat atgctggttg tagaccccaa aagtaaggaa
     2221 gaagataaac acctgaaatt tcgtatttct catgaattag atagtgcatc ttctgaggtc
     2281 aattagggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     2341 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2401 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2521 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     2581 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2641 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     2701 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     2761 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     2821 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     2881 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     2941 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3001 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3061 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3121 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     3181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3241 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3301 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3361 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3421 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3481 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3541 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3601 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3661 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     3721 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3781 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3841 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3901 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3961 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4021 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     4081 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4141 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4201 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4261 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4321 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4381 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4441 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     4501 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     4561 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     4621 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     4681 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     4741 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     4801 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4861 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     4921 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     4981 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5041 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5101 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5161 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5221 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5281 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5581 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
