LOCUS V020796 5369 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020796 VERSION V020796 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 625..1263 /label="SOST(NM_025237)" /note="SOST(NM_025237)" /gene="SOST" CDS 1315..2034 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 2157..2278 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2285..2740) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2767..2871 /gene="bla" /label="AmpR promoter" promoter 2873..3230 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3265..4059 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4291..4338 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 4667..5255 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatgcag ctcccactgg ccctgtgtct cgtctgcctg 661 ctggtacaca cagccttccg tgtagtggag ggccaggggt ggcaggcgtt caagaatgat 721 gccacggaaa tcatccccga gctcggagag taccccgagc ctccaccgga gctggagaac 781 aacaagacca tgaaccgggc ggagaacgga gggcggcctc cccaccaccc ctttgagacc 841 aaagacgtgt ccgagtacag ctgccgcgag ctgcacttca cccgctacgt gaccgatggg 901 ccgtgccgca gcgccaagcc ggtcaccgag ctggtgtgct ccggccagtg cggcccggcg 961 cgcctgctgc ccaacgccat cggccgcggc aagtggtggc gacctagtgg gcccgacttc 1021 cgctgcatcc ccgaccgcta ccgcgcgcag cgcgtgcagc tgctgtgtcc cggtggtgag 1081 gcgccgcgcg cgcgcaaggt gcgcctggtg gcctcgtgca agtgcaagcg cctcacccgc 1141 ttccacaacc agtcggagct caaggacttc gggaccgagg ccgctcggcc gcagaagggc 1201 cggaagccgc ggccccgcgc ccggagcgcc aaagccaacc aggccgagct ggagaacgcc 1261 taccgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg 1321 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 1381 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 1441 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 1501 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 1561 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 1621 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 1681 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 1741 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 1801 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 1861 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 1921 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 1981 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaagcggc 2041 cgcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa 2101 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact 2161 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 2221 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag 2281 gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc 2341 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 2401 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 2461 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 2521 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 2581 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 2641 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 2701 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga 2761 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 2821 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg 2881 gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 2941 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3001 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3061 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3121 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 3181 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa 3241 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 3301 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 3361 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 3421 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 3481 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 3541 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 3601 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 3661 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 3721 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 3781 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 3841 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 3901 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 3961 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 4021 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 4081 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 4141 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 4201 gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg 4261 agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg 4321 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata 4381 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc 4441 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca 4501 tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 4561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 4621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 4681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 4741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 4801 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 4861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 4921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 4981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 5041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 5101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 5161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 5221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 5281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 5341 ctgtggataa ccgtattacc gccatgcat //