LOCUS       V018698                 8105 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018698
VERSION     V018698
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..4035
                     /label="SMG7(NM_173156)"
                     /note="SMG7(NM_173156)"
                     /gene="SMG7"
     CDS             4051..4770
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    4893..5014
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(5021..5476)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5503..5607
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        5609..5966
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6001..6795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7027..7074
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      7403..7991
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatgagc ctgcagagcg cgcagtacct ccggcaggca
      661 gaagtcctga aggctgacat gacagattct aagctgggtc cagctgaagt ctggacatcc
      721 aggcaggctc tgcaggacct gtaccagaaa atgctagtta ccgatttgga atacgcttta
      781 gacaagaaag tagaacagga tctctggaat cacgccttta agaatcagat cacaacactg
      841 caaggccagg caaagaatcg agcaaatccg aatcggagtg aagttcaggc aaacctttct
      901 ctgttcctag aggcagctag tggcttctat actcagttat tacaagaact gtgtacagta
      961 tttaatgtag atttaccatg ccgtgtgaag tcttcccaat tgggaattat cagcaataaa
     1021 cagacgcata ccagcgccat agtgaagcca cagtctagct cctgttccta tatctgccag
     1081 cactgcctcg tccaccttgg agacattgct cgatacagaa accagaccag ccaggcagag
     1141 tcctactata ggcatgcagc tcagcttgtc ccctccaatg gtcagcctta taatcagttg
     1201 gctatcttag cttcttccaa aggagaccat ctgaccacaa ttttctacta ctgcagaagc
     1261 attgctgtga agttcccttt cccagctgcc tccactaatc tgcaaaaagc actttctaaa
     1321 gcactggaaa gccgagatga ggtgaaaacc aagtggggtg tttctgactt catcaaggcc
     1381 tttattaaat tccacggtca tgtgtacctg agtaagagct tggaaaagtt gagccctctt
     1441 cgagagaaat tggaagaaca gtttaagagg ctgctattcc aaaaagcttt caactctcag
     1501 cagttagttc atgtcactgt cattaacctg tttcaacttc atcaccttcg tgactttagc
     1561 aatgaaaccg agcagcacac ttatagccaa gatgagcagc tatgttggac acagttgctg
     1621 gccctcttta tgtcttttct tggcatcctg tgcaagtgtc ctctacagaa tgagtctcag
     1681 gaggagtcct acaatgccta tcctcttcca gcagtcaagg tctccatgga ctggctaaga
     1741 ctcagaccca gggtctttca ggaggcagtg gtggatgaaa gacagtacat ttggccctgg
     1801 ttgatttctc ttctgaatag tttccatccc catgaagagg acctctcaag tattagtgcg
     1861 acaccacttc cagaggagtt tgaattacaa ggatttttgg cattgagacc ttctttcagg
     1921 aacttggatt tttccaaagg tcaccagggt attacagggg acaaagaagg ccagcaacga
     1981 cgaatacgac agcaacgctt gatctctata ggcaaatgga ttgctgataa tcagccaagg
     2041 ctgattcagt gtgaaaatga ggtagggaaa ttgttgttta tcacagaaat cccagaatta
     2101 atactggaag accccagtga agccaaagag aacctcattc tgcaagaaac atctgtgata
     2161 gagtcgctgg ctgcagatgg gagcccaggg ctaaaatcag tgctatctac aagccgaaat
     2221 ttaagcaaca actgtgacac aggagagaag ccagtggtta ccttcaaaga aaacattaag
     2281 acacgagaag tgaacagaga ccaaggaaga agttttcctc ccaaagaggt gagaagggac
     2341 tatagcaaag gaataactgt aactaagaat gatggaaaga aggacaacaa caagaggaaa
     2401 actgaaacca agaaatgcac cttagaaaag ttacaggaaa caggaaagca gaatgtggca
     2461 gtgcaggtaa aatcccagac agaactaaga aagactccag tgtctgaagc cagaaaaaca
     2521 cctgtaactc aaaccccaac tcaagcaagt aactcccagt tcatccccat tcatcaccct
     2581 ggagccttcc ctcctcttcc cagcaggcca gggtttccgc ccccaacata tgttatcccc
     2641 ccgcctgtgg cattttctat gggctcaggt tacaccttcc cagctggtgt ttctgtccca
     2701 ggaacctttc ttcagcctac agctcactct ccagcaggaa accaggtgca agctgggaaa
     2761 cagtcccaca ttccttacag ccagcaacgg ccctctggac cagggccaat gaaccaggga
     2821 cctcaacaat cacagccacc ttcccagcaa ccccttacat ctttaccagc tcagccaaca
     2881 gcacagtcta caagccagct gcaggttcaa gctctaactc agcaacaaca atcccctaca
     2941 aaagctgtgc cggctttggg gaaaagcccg cctcaccact ctggattcca gcagtatcaa
     3001 caggcagatg cctccaaaca gctgtggaat ccccctcagg ttcaaggccc attagggaaa
     3061 attatgcctg tgaaacagcc ctactacctt cagacccaag accccataaa actgtttgag
     3121 ccgtcattgc aacctcctgt aatgcagcag cagcctctag aaaaaaaaat gaagcctttt
     3181 cccatggagc catataacca taatccctca gaagtcaagg tcccagaatt ctactgggat
     3241 tcttcctaca gcatggctga taacagatct gtaatggcac agcaagcaaa catagaccgc
     3301 aggggcaaac ggtcaccagg agtcttccgt ccagagcagg atcctgtacc cagaatgccg
     3361 tttgagaaat ccttattgga gaagccctca gagctcatgt cacattcatc ctctttcctg
     3421 tccctcaccg gattctctct caatcaggaa agatacccaa ataatagtat gttcaatgag
     3481 gtatatggga aaaacctgac atccagctcc aaagcagaac tcagtccctc aatggccccc
     3541 caggaaacat ctctgtattc cctttttgaa gggactccgt ggtctccatc acttcctgcc
     3601 agttcagatc attcaacacc agccagccag tctcctcatt cctctaaccc aagcagccta
     3661 cccagctctc ctccaacaca caaccataat tctgttccat tctccaattt tggacccatt
     3721 gggactccag ataacaggga tagaaggact gcagatcggt ggaaaactga taagccagcc
     3781 atgggtgggt ttggcattga ttatctctca gcaacgtcat cctctgagag cagttggcat
     3841 caggccagca ctccgagtgg cacctggaca ggccatggcc cttccatgga ggattcctct
     3901 gctgtcctca tggaaagcct aaagtctatc tggtccagtt ccatgatgca tcctggacct
     3961 tctgctctgg agcagctgtt aatgcagcag aagcagaaac agcaacgggg acaaggcacc
     4021 atgaaccctc cacactcacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
     4081 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4141 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4201 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     4261 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4321 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4381 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4441 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4501 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4561 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4621 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4681 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4741 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
     4801 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4861 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4921 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4981 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     5041 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     5101 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     5161 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     5221 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     5281 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     5341 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     5401 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     5461 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5521 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5581 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     5641 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5701 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     5761 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     5821 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5881 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5941 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     6001 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     6061 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     6121 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6181 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     6241 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6301 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6361 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6421 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6481 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     6541 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6601 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6661 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6721 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6781 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     6841 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6901 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6961 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     7021 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     7081 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     7141 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     7201 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     7261 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7321 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7381 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7441 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7501 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7561 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7621 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7681 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7741 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7801 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7861 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7921 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7981 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8041 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     8101 tgcat
//
