LOCUS       V026624                 9710 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026624
VERSION     V026624
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1320
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             1378..6318
                     /label="SMARCA4(NM_001128844)"
                     /note="SMARCA4(NM_001128844)"
                     /gene="SMARCA4"
     CDS             6325..6366
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     polyA_signal    6498..6619
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(6626..7081)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        7108..7212
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        7214..7571
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             7606..8400
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    8632..8679
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      9008..9596
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tggaggtgga ggtagtggag gaggcggaag caagcttatg
     1381 tccactccag acccacccct gggcggaact cctcggccag gtccttcccc gggccctggc
     1441 ccttcccctg gagccatgct gggccctagc ccgggtccct cgccgggctc cgcccacagc
     1501 atgatggggc ccagcccagg gccgccctca gcaggacacc ccatccccac ccaggggcct
     1561 ggagggtacc ctcaggacaa catgcaccag atgcacaagc ccatggagtc catgcatgag
     1621 aagggcatgt cggacgaccc gcgctacaac cagatgaaag gaatggggat gcggtcaggg
     1681 ggccatgctg ggatggggcc cccgcccagc cccatggacc agcactccca aggttacccc
     1741 tcgcccctgg gtggctctga gcatgcctct agtccagttc cagccagtgg cccgtcttcg
     1801 gggccccaga tgtcttccgg gccaggaggt gccccgctgg atggtgctga cccccaggcc
     1861 ttggggcagc agaaccgggg cccaacccca tttaaccaga accagctgca ccagctcaga
     1921 gctcagatca tggcctacaa gatgctggcc agggggcagc ccctccccga ccacctgcag
     1981 atggcggtgc agggcaagcg gccgatgccc gggatgcagc agcagatgcc aacgctacct
     2041 ccaccctcgg tgtccgcaac aggacccggc cctggccctg gccctggccc cggcccgggt
     2101 cccggcccgg cacctccaaa ttacagcagg cctcatggta tgggagggcc caacatgcct
     2161 cccccaggac cctcgggcgt gccccccggg atgccaggcc agcctcctgg agggcctccc
     2221 aagccctggc ctgaaggacc catggcgaat gctgctgccc ccacgagcac ccctcagaag
     2281 ctgattcccc cgcagccaac gggccgccct tcccccgcgc cccctgccgt cccacccgcc
     2341 gcctcgcccg tgatgccacc gcagacccag tcccccgggc agccggccca gcccgcgccc
     2401 atggtgccac tgcaccagaa gcagagccgc atcaccccca tccagaagcc gcggggcctc
     2461 gaccctgtgg agatcctgca ggagcgcgag tacaggctgc aggctcgcat cgcacaccga
     2521 attcaggaac ttgaaaacct tcccgggtcc ctggccgggg atttgcgaac caaagcgacc
     2581 attgagctca aggccctcag gctgctgaac ttccagaggc agctgcgcca ggaggtggtg
     2641 gtgtgcatgc ggagggacac agcgctggag acagccctca atgctaaggc ctacaagcgc
     2701 agcaagcgcc agtccctgcg cgaggcccgc atcactgaga agctggagaa gcagcagaag
     2761 atcgagcagg agcgcaagcg ccggcagaag caccaggaat acctcaatag cattctccag
     2821 catgccaagg atttcaagga atatcacaga tccgtcacag gcaaaatcca gaagctgacc
     2881 aaggcagtgg ccacgtacca tgccaacacg gagcgggagc agaagaaaga gaacgagcgg
     2941 atcgagaagg agcgcatgcg gaggctcatg gctgaagatg aggaggggta ccgcaagctc
     3001 atcgaccaga agaaggacaa gcgcctggcc tacctcttgc agcagacaga cgagtacgtg
     3061 gctaacctca cggagctggt gcggcagcac aaggctgccc aggtcgccaa ggagaaaaag
     3121 aagaaaaaga aaaagaagaa ggcagaaaat gcagaaggac agacgcctgc cattgggccg
     3181 gatggcgagc ctctggacga gaccagccag atgagcgacc tcccggtgaa ggtgatccac
     3241 gtggagagtg ggaagatcct cacaggcaca gatgccccca aagccgggca gctggaggcc
     3301 tggctcgaga tgaacccggg gtatgaagta gctccgaggt ctgatagtga agaaagtggc
     3361 tcagaagaag aggaagagga ggaggaggaa gagcagccgc aggcagcaca gcctcccacc
     3421 ctgcccgtgg aggagaagaa gaagattcca gatccagaca gcgatgacgt ctctgaggtg
     3481 gacgcgcggc acatcattga gaatgccaag caagatgtcg atgatgaata tggcgtgtcc
     3541 caggcccttg cacgtggcct gcagtcctac tatgccgtgg cccatgctgt cactgagaga
     3601 gtggacaagc agtcagcgct tatggtcaat ggtgtcctca aacagtacca gatcaaaggt
     3661 ttggagtggc tggtgtccct gtacaacaac aacctgaacg gcatcctggc cgacgagatg
     3721 ggcctgggga agaccatcca gaccatcgcg ctcatcacgt acctcatgga gcacaaacgc
     3781 atcaatgggc ccttcctcat catcgtgcct ctctcaacgc tgtccaactg ggcgtacgag
     3841 tttgacaagt gggccccctc cgtggtgaag gtgtcttaca agggatcccc agcagcaaga
     3901 cgggcctttg tcccccagct ccggagtggg aagttcaacg tcttgctgac gacgtacgag
     3961 tacatcatca aagacaagca catcctcgcc aagatccgtt ggaagtacat gattgtggac
     4021 gaaggtcacc gcatgaagaa ccaccactgc aagctgacgc aggtgctcaa cacgcactat
     4081 gtggcacccc gccgcctgct gctgacgggc acaccgctgc agaacaagct tcccgagctc
     4141 tgggcgctgc tcaacttcct gctgcccacc atcttcaaga gctgcagcac cttcgagcag
     4201 tggtttaacg caccctttgc catgaccggg gaaaaggtgg acctgaatga ggaggaaacc
     4261 attctcatca tccggcgtct ccacaaagtg ctgcggccct tcttgctccg acgactcaag
     4321 aaggaagtcg aggcccagtt gcccgaaaag gtggagtacg tcatcaagtg cgacatgtct
     4381 gcgctgcagc gagtgctcta ccgccacatg caggccaagg gcgtgctgct gactgatggc
     4441 tccgagaagg acaagaaggg caaaggcggc accaagaccc tgatgaacac catcatgcag
     4501 ctgcggaaga tctgcaacca cccctacatg ttccagcaca tcgaggagtc cttttccgag
     4561 cacttggggt tcactggcgg cattgtccaa gggctggacc tgtaccgagc ctcgggtaaa
     4621 tttgagcttc ttgatagaat tcttcccaaa ctccgagcaa ccaaccacaa agtgctgctg
     4681 ttctgccaaa tgacctccct catgaccatc atggaagatt actttgcgta tcgcggcttt
     4741 aaatacctca ggcttgatgg aaccacgaag gcggaggacc ggggcatgct gctgaaaacc
     4801 ttcaacgagc ccggctctga gtacttcatc ttcctgctca gcacccgggc tggggggctc
     4861 ggcctgaacc tccagtcggc agacactgtg atcatttttg acagcgactg gaatcctcac
     4921 caggacctgc aagcgcagga ccgagcccac cgcatcgggc agcagaacga ggtgcgtgtg
     4981 ctccgcctct gcaccgtcaa cagcgtggag gagaagatcc tagctgcagc caagtacaag
     5041 ctcaacgtgg accagaaggt gatccaggcc ggcatgttcg accagaagtc ctccagccat
     5101 gagcggcgcg ccttcctgca ggccatcctg gagcacgagg agcaggatga gagcagacac
     5161 tgcagcacgg gcagcggcag tgccagcttc gcccacactg cccctccgcc agcgggcgtc
     5221 aaccccgact tggaggagcc acctctaaag gaggaagacg aggtgcccga cgacgagacc
     5281 gtcaaccaga tgatcgcccg gcacgaggag gagtttgatc tgttcatgcg catggacctg
     5341 gaccgcaggc gcgaggaggc ccgcaacccc aagcggaagc cgcgcctcat ggaggaggac
     5401 gagctcccct cgtggatcat caaggacgac gcggaggtgg agcggctgac ctgtgaggag
     5461 gaggaggaga agatgttcgg ccgtggctcc cgccaccgca aggaggtgga ctacagcgac
     5521 tcactgacgg agaagcagtg gctcaaggcc atcgaggagg gcacgctgga ggagatcgaa
     5581 gaggaggtcc ggcagaagaa atcatcacgg aagcgcaagc gagacagcga cgccggctcc
     5641 tccaccccga ccaccagcac ccgcagccgc gacaaggacg acgagagcaa gaagcagaag
     5701 aagcgcgggc ggccgcctgc cgagaaactc tcccctaacc cacccaacct caccaagaag
     5761 atgaagaaga ttgtggatgc cgtgatcaag tacaaggaca gcagcagtgg acgtcagctc
     5821 agcgaggtct tcatccagct gccctcgcga aaggagctgc ccgagtacta cgagctcatc
     5881 cgcaagcccg tggacttcaa gaagataaag gagcgcattc gcaaccacaa gtaccgcagc
     5941 ctcaacgacc tagagaagga cgtcatgctc ctgtgccaga acgcacagac cttcaacctg
     6001 gagggctccc tgatctatga agactccatc gtcttgcagt cggtcttcac cagcgtgcgg
     6061 cagaaaatcg agaaggagga tgacagtgaa ggcgaggaga gtgaggagga ggaagagggc
     6121 gaggaggaag gctccgaatc cgaatctcgg tccgtcaaag tgaagatcaa gcttggccgg
     6181 aaggagaagg cacaggaccg gctgaagggc ggccggcggc ggccgagccg agggtcccga
     6241 gccaagccgg tcgtgagtga cgatgacagt gaggaggaac aagaggagga ccgctcagga
     6301 agtggcagcg aagaagacgg taccggcaag ccgatcccta accctctgct gggcctggac
     6361 agcacttagg atccaccgga tctagataac tgatcataat cagccatacc acatttgtag
     6421 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     6481 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6601 aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     6661 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     6721 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     6781 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     6841 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     6901 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     6961 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     7021 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     7081 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7141 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7201 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     7261 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7321 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7381 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7441 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7501 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7561 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     7621 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     7681 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     7741 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     7801 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     7861 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     7921 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     7981 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     8041 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     8101 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     8161 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     8221 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     8281 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     8341 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     8401 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     8461 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     8521 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     8581 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     8641 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     8701 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     8761 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     8821 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     8881 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8941 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     9001 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     9061 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9121 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9661 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
