LOCUS       V020738                 6231 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020738
VERSION     V020738
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1384..2886
                     /label="SLC7A11(NM_014331)"
                     /note="SLC7A11(NM_014331)"
                     /gene="SLC7A11"
     polyA_signal    3019..3140
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3147..3602)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3629..3733
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3735..4092
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4127..4921
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5153..5200
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5529..6117
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttatggtca gaaagcctgt tgtgtccacc atctccaaag gaggttacct gcagggaaat
     1441 gttaacggga ggctgccttc cctgggcaac aaggagccac ctgggcagga gaaagtgcag
     1501 ctgaagagga aagtcacttt actgagggga gtctccatta tcattggcac catcattgga
     1561 gcaggaatct tcatctctcc taagggcgtg ctccagaaca cgggcagcgt gggcatgtct
     1621 ctgaccatct ggacggtgtg tggggtcctg tcactatttg gagctttgtc ttatgctgaa
     1681 ttgggaacaa ctataaagaa atctggaggt cattacacat atattttgga agtctttggt
     1741 ccattaccag cttttgtacg agtctgggtg gaactcctca taatacgccc tgcagctact
     1801 gctgtgatat ccctggcatt tggacgctac attctggaac cattttttat tcaatgtgaa
     1861 atccctgaac ttgcgatcaa gctcattaca gctgtgggca taactgtagt gatggtccta
     1921 aatagcatga gtgtcagctg gagcgcccgg atccagattt tcttaacctt ttgcaagctc
     1981 acagcaattc tgataattat agtccctgga gttatgcagc taattaaagg tcaaacgcag
     2041 aactttaaag acgccttttc aggaagagat tcaagtatta cgcggttgcc actggctttt
     2101 tattatggaa tgtatgcata tgctggctgg ttttacctca actttgttac tgaagaagta
     2161 gaaaaccctg aaaaaaccat tccccttgca atatgtatat ccatggccat tgtcaccatt
     2221 ggctatgtgc tgacaaatgt ggcctacttt acgaccatta atgctgagga gctgctgctt
     2281 tcaaatgcag tggcagtgac cttttctgag cggctactgg gaaatttctc attagcagtt
     2341 ccgatctttg ttgccctctc ctgctttggc tccatgaacg gtggtgtgtt tgctgtctcc
     2401 aggttattct atgttgcgtc tcgagagggt caccttccag aaatcctctc catgattcat
     2461 gtccgcaagc acactcctct accagctgtt attgttttgc accctttgac aatgataatg
     2521 ctcttctctg gagacctcga cagtcttttg aatttcctca gttttgccag gtggcttttt
     2581 attgggctgg cagttgctgg gctgatttat cttcgataca aatgcccaga tatgcatcgt
     2641 cctttcaagg tgccactgtt catcccagct ttgttttcct tcacatgcct cttcatggtt
     2701 gccctttccc tctattcgga cccatttagt acagggattg gcttcgtcat cactctgact
     2761 ggagtccctg cgtattatct ctttattata tgggacaaga aacccaggtg gtttagaata
     2821 atgtcggaga aaataaccag aacattacaa ataatactgg aagttgtacc agaagaagat
     2881 aagttatgag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     2941 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     3001 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3061 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3121 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     3181 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3241 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     3301 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     3361 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     3421 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     3481 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     3541 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     3601 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3661 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3721 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     3781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3841 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3901 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3961 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4021 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4081 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     4141 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     4201 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     4261 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     4321 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     4381 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     4441 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     4501 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     4561 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     4621 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
     4681 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     4741 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     4801 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     4861 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     4921 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     4981 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     5041 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
     5101 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     5161 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     5221 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     5281 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
     5341 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
     5401 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5461 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     5521 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     5581 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     5641 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     5701 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     5761 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     5821 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     5881 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5941 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6001 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6061 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6121 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6181 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
