LOCUS       V022077                 5946 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V022077
VERSION     V022077
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1393..2601
                     /label="SEC14L2(NM_012429)"
                     /note="SEC14L2(NM_012429)"
                     /gene="SEC14L2"
     polyA_signal    2734..2855
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(2862..3317)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3344..3448
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3450..3807
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3842..4636
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4868..4915
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5244..5832
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttcgaattc tgatgagcgg cagagtcggc gatctgagcc ccaggcagaa ggaggcattg
     1441 gccaagtttc gggagaatgt ccaggatgtg ctgccggccc tgccgaatcc agatgactat
     1501 tttctcctgc gttggctccg agccagaagc ttcgacctgc agaagtcgga ggccatgctc
     1561 cggaagcatg tggagttccg aaagcaaaag gacattgaca acatcattag ctggcagcct
     1621 ccagaggtga tccaacagta tctgtcaggg ggtatgtgtg gctatgacct ggatggctgc
     1681 ccagtctggt acgacataat tggacctctg gatgccaagg gtctgctgtt ctcagcctcc
     1741 aaacaggacc tgctgaggac caagatgcgg gagtgtgagc tgcttctgca agagtgtgcc
     1801 caccagacca caaagttggg gaggaaggtg gagaccatca ccataattta tgactgcgag
     1861 gggcttggcc tcaagcatct ctggaagcct gctgtggagg cctatggaga gtttctctgc
     1921 atgtttgagg aaaattatcc cgaaacactg aagcgtcttt ttgttgttaa agcccccaaa
     1981 ctgtttcctg tggcctataa cctcatcaaa cccttcctga gtgaggacac tcgtaagaag
     2041 atcatggtcc tgggagcaaa ttggaaggag gttttactga aacatatcag ccctgaccag
     2101 gtgcctgtgg agtatggggg caccatgact gaccctgatg gaaaccccaa gtgcaaatcc
     2161 aagatcaact acgggggtga catccccagg aagtattatg tgcgagacca ggtgaaacag
     2221 cagtatgaac acagcgtgca gatttcccgt ggctcctccc accaagtgga gtatgagatc
     2281 ctcttccctg gctgtgtcct caggtggcag tttatgtcag atggagcgga tgttggtttt
     2341 gggattttcc tgaagaccaa gatgggagag aggcagcggg caggggagat gacagaggtg
     2401 ctgcccaacc agaggtacaa ctcccacctg gtccctgaag atgggaccct cacctgcagt
     2461 gatcctggca tctatgtcct gcggtttgac aacacctaca gcttcattca tgccaagaag
     2521 gtcaatttca ctgtggaggt cctgcttcca gacaaagcct cagaagagaa gatgaaacag
     2581 ctgggggcag gcaccccgaa ataaggatcc accggatcta gataactgat cataatcagc
     2641 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2701 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2821 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     2881 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2941 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3001 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     3061 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     3121 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     3181 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     3241 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     3301 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3421 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     3481 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3541 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     3601 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     3661 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3721 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3781 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     3841 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3901 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3961 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     4021 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     4081 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     4141 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     4201 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     4261 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     4321 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     4381 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     4441 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     4501 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     4561 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     4621 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     4681 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4741 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4801 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     4861 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     4921 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     4981 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     5041 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     5101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5941 atgcat
//
