LOCUS V025839 7921 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V025839 VERSION V025839 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 603..1301 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric blue fluorescent protein" /transl_table=1 /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC DLPSKLGHKLN" /label="TagBFP" CDS 1353..4541 /label="RNF213(NM_020954)" /note="RNF213(NM_020954)" /gene="RNF213" polyA_signal 4709..4830 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(4837..5292) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5319..5423 /gene="bla" /label="AmpR promoter" promoter 5425..5782 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 5817..6611 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 6843..6890 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 7219..7807 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca 601 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg 661 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga 721 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta 781 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct 841 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg 901 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca 961 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct 1021 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca 1081 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat 1141 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg 1201 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca 1261 gatactgcga cctccctagc aaactggggc acaagcttaa tggcggcgga gggagtggag 1321 gagggggaag cggaggcgga gggagtctcg agatggagtg tccttcgtgc cagcatgtct 1381 ccaaggagga aacccccaag ttctgcagcc agtgcggaga gaggctgcct cctgcagccc 1441 ccatagcaga ttctgagaac aataactcca caatggcgtc ggcctcggag ggtgaaatgg 1501 agtgtgggca ggagctgaag gaggaagggg gcccgtgctt gttcccgggc tcagacagtt 1561 ggcaagaaaa ccccgaggag ccctgttcca aagcctcctg gaccgtccaa gaaagcaaaa 1621 agaagaaaag gaagaagaaa aagaagggga acaagtccgc ttcctcagag ctggcttcct 1681 tgcccctttc tcctgccagc ccctgtcacc tgactttgct ttcaaacccg tggcctcagg 1741 acacagccct gccccacagc caagcccagc agagtggccc cactggccag ccgagccagc 1801 ccccaggcac agccaccacg ccactggagg gtgacggcct ctccgcgccc accgaggttg 1861 gcgacagccc cctgcaggcc caggctttgg gagaggcagg agtggccaca ggaagtgagg 1921 ctcagagcag cccgcaattc caggaccaca cggaagggga ggaccaggac gcttccatcc 1981 cctctggggg cagaggcctg tcccaggagg ggaccggtcc ccccacctct gctggtgaag 2041 gccattctag gactgaagat gctgcccagg agctcctgtt gcctgagtca aaaggaggca 2101 gctctgagcc cgggacagaa ctgcagacca ccgagcaaca ggcaggggcc tcagcctcta 2161 tggcagttga tgctgtagct gagccagcca atgcagttaa aggggccggg aaggaaatga 2221 aagagaagac ccagagaatg aaacagccac cagcaaccac tcctcctttc aaaacacact 2281 gccaggaagc tgagaccaag accaaggacg agatggctgc tgctgaagaa aaagtcggta 2341 agaatgaaca aggggagcct gaagacctca agaagccaga ggggaagaac agaagtgcag 2401 ctgctgtgaa aaacgagaag gagcaaaaaa accaggaagc agatgtccag gaagtgaagg 2461 caagcacgct gagcccgggt ggaggagtca ccgtgttctt ccacgccatc atctctcttc 2521 atttcccatt caatcctgac ctccataaag tcttcatcag aggaggagaa gaatttgggg 2581 agtcaaaatg ggacagcaat atctgtgagc tgcactacac cagagacttg ggtcatgacc 2641 gcgttcttgt tgaaggcatt gtctgcattt ccaagaagca cctagataaa tacattcctt 2701 acaagtacgt catttataat ggggaatctt ttgagtatga gttcatttac aagcaccagc 2761 agaagaaggg cgagtacgtc aaccgctgtc tgttcataaa atcttcactt ctgggctcag 2821 gagactggca tcagtactat gacatagttt atatgaagcc tcatgggaga ctccagaaag 2881 tcatgaacca catcacagac gggccgagga aggacctggt gaaggggaag cagattgccg 2941 ctgcgctcat gctggacagc accttcagca tcctgcagac ctgggacacc atcaacctga 3001 acagcttctt cacccagttc gagcagtttt gctttgtcct gcaacagcct atgatttatg 3061 aaggacaggc acagctgtgg accgatttgc agtacaggga gaaagaggtg aagagatacc 3121 tgtggcaaca tctgaaaaaa cacgtggtac cattgccgga cggaaaaagc acggactttt 3181 tgcctgtgga ctgcccagtg aggagtaaac tgaaaacagg cctgattgtc ctttttgtag 3241 tggaaaaaat tgagctttta ttagaaggca gcctggactg gttgtgtcac ctcctaacct 3301 cagatgccag ctcaccagat gagtttcacc gtgacctaag ccacatcctt gggatacctc 3361 agagctggcg gctgtacctg gtgaacctgt gccaaagatg catggacaca aggacgtaca 3421 cctggctggg cgccctgcct gtcctgcact gctgtatgga gctggccccg cggcacaagg 3481 atgcctggag acagcctgag gacacctggg ccgctctgga gggactctcc ttctcaccgt 3541 tccgggaaca aatgctagat acgagttccc tacttcagtt tatgagagag aagcagcatt 3601 tgctgagcat agacgagcct ctcttccggt cctggtttag tctgctacct ctgagtcacc 3661 tggttatgta tatggaaaac ttcattgagc acctgggtcg ttttcctgct catatcctgg 3721 actgtctttc agggatttac taccggcttc cgggacttga gcaagtcttg aatacgcagg 3781 atgttcagga tgttcagaac gttcagaaca ttttagaaat gctgttgcga ctcctggaca 3841 cttaccggga caagattccc gaggaggcct tgtcaccatc ctacctgact gtgtgtctga 3901 aactgcatga agccatctgc agcagcacaa agctacttaa gttttacgag ctgccagcct 3961 tatctgccga gattgtctgc agaatgatta gacttctatc tctggtggat tctgcaggac 4021 agagagatga aactggaaat aattcagtcc aaacagtctt ccaagggacc cttgctgcta 4081 cgaaaaggtg gctccgagaa gtttttacaa agaacatgct cacatcttca ggtgcctcat 4141 tcacatacgt caaggaaatt gaggtctgga ggcggctggt ggaaatccaa ttccccgcgg 4201 agcatggctg gaaggagtcg ttgctgggag acatggaatg gaggctcaca aaggaggaac 4261 ccctctccca gatcactgcc tactgcaata gttgctggga caccaaaggc ttagaggaca 4321 gtgtggccaa gaccttcgag aaatgcatca ttgaagccgt gagctcagcc tgccaggtga 4381 acaatctctc ctcctgggaa acggattcgg gctcacagct gtgttctgcc atgacccagc 4441 taagggctat gaagcacccg ctgggtctca gctcctccgc taactcagag attgggaagt 4501 gggcaccctc ctccctcgcc aagggcaatg gcgctgaaat ctaggaattc tgcagtcgac 4561 ggtaccgcgg gcccgggatc caccggtgcg gccgcgactc tagatcataa tcagccatac 4621 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 4681 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 4741 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 4801 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt 4861 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 4921 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 4981 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 5041 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 5101 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 5161 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 5221 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 5281 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 5341 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 5401 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca 5461 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 5521 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 5581 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 5641 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 5701 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 5761 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga 5821 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 5881 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 5941 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag 6001 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 6061 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 6121 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 6181 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 6241 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 6301 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg 6361 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 6421 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 6481 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 6541 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 6601 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 6661 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 6721 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 6781 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 6841 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg 6901 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata 6961 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct 7021 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta 7081 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 7141 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 7201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 7261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 7321 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 7381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 7441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 7501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 7561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 7621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 7681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 7741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 7801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 7861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca 7921 t //