LOCUS V024851 5946 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024851 VERSION V024851 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1384..2601 /label="RING1(NM_002931)" /note="RING1(NM_002931)" /gene="RING1" polyA_signal 2734..2855 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2862..3317) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3344..3448 /gene="bla" /label="AmpR promoter" promoter 3450..3807 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3842..4636 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4868..4915 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5244..5832 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag 1381 cttatgacga cgccggcgaa tgcccagaat gccagcaaaa cgtgggaact gagtctgtat 1441 gagctgcacc ggaccccgca ggaagccata atggatggca cagagattgc tgtttcccct 1501 cggtcactgc attcagaact catgtgccct atctgcctgg acatgctgaa gaatacgatg 1561 accaccaagg agtgcctcca cagattctgc tctgactgca ttgtcacagc cctacggagc 1621 gggaacaagg agtgtcctac ctgccgaaag aagctggtgt ccaagcgatc cctacggcca 1681 gaccccaact ttgatgccct gatctctaag atctatccta gccgggagga atacgaggcc 1741 catcaagacc gagtgcttat ccgcctgagc cgcctgcaca accagcaggc attgagctcc 1801 agcattgagg aggggctacg catgcaggcc atgcacaggg cccagcgtgt gaggcggccg 1861 ataccagggt cagatcagac cacaacgatg agtggggggg aaggagagcc cggggaggga 1921 gaaggggatg gagaagatgt gagctcagac tccgcccctg actctgcccc aggccctgct 1981 cccaagcgac cccgtggagg gggcgcaggg gggagcagtg tagggacagg gggaggcggc 2041 actggtgggg tgggtggggg tgccggttcg gaagactctg gtgaccgggg agggactctg 2101 ggagggggaa cgctgggccc cccaagccct cctggggccc ccagcccccc agagccaggt 2161 ggagaaattg agctcgtgtt ccggccccac cccctgctcg tggagaaggg agaatactgc 2221 cagacgaggt atgtgaagac aactgggaat gccacagtgg accacctctc caagtacttg 2281 gccctgcgca ttgccctcga gcggaggcaa cagcaggaag caggggagcc aggagggcct 2341 ggagggggcg cctctgacac cggaggacct gatgggtgtg gcggggaggg tgggggtgcc 2401 ggaggaggtg acggtcctga ggagcctgct ttgcccagcc tggagggcgt cagtgaaaag 2461 cagtacacca tctacatcgc acctggaggc ggggcgttca cgacgttgaa tggctcgctg 2521 accctggagc tggtgaatga gaaattctgg aaggtgtccc ggccactgga gctgtgctat 2581 gctcccacca aggatccaaa gtgaggatcc accggatcta gataactgat cataatcagc 2641 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac 2701 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt 2761 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 2821 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat 2881 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga 2941 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc 3001 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 3061 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc 3121 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg 3181 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag 3241 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc 3301 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 3361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 3421 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg 3481 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 3541 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 3601 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 3661 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 3721 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 3781 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg 3841 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 3901 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 3961 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 4021 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 4081 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 4141 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 4201 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 4261 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 4321 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga 4381 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 4441 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 4501 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 4561 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 4621 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac 4681 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 4741 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 4801 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc 4861 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa 4921 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc 4981 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca 5041 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata 5101 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 5161 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 5221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 5281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 5341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 5401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 5461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 5521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 5581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 5641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 5701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 5761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 5821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct 5881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 5941 atgcat //