LOCUS       V018540                 6383 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018540
VERSION     V018540
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1480..3039
                     /label="RELA(NM_001404662)"
                     /note="RELA(NM_001404662)"
                     /gene="RELA"
     polyA_signal    3171..3292
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3299..3754)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3781..3885
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3887..4244
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4279..5073
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5305..5352
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5681..6269
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag
     1381 cttcgaattg acgaactgtt ccccctcatc ttcccggcag agccagccca ggcctctggc
     1441 ccctatgtgg agatcattga gcagcccaag cagcggggca tgcgcttccg ctacaagtgc
     1501 gaggggcgct ccgcgggcag catcccaggc gagaggagca cagataccac caagacccac
     1561 cccaccatca agatcaatgg ctacacagga ccagggacag tgcgcatctc cctggtcacc
     1621 aaggaccctc ctcaccggcc tcacccccac gagcttgtag gaaaggactg ccgggatggc
     1681 ttctatgagg ctgagctctg cccggaccgc tgcatccaca gtttccagaa cctgggaatc
     1741 cagtgtgtga agaagcggga cctggagcag gctatcagtc agcgcatcca gaccaacaac
     1801 aaccccttcc aagttcctat agaagagcag cgtggggact acgacctgaa tgctgtgcgg
     1861 ctctgcttcc aggtgacagt gcgggaccca tcaggcaggc ccctccgcct gccgcctgtc
     1921 ctttctcatc ccatctttga caatcgtgcc cccaacactg ccgagctcaa gatctgccga
     1981 gtgaaccgaa actctggcag ctgcctcggt ggggatgaga tcttcctact gtgtgacaag
     2041 gtgcagaaag aggacattga ggtgtatttc acgggaccag gctgggaggc ccgaggctcc
     2101 ttttcgcaag ctgatgtgca ccgacaagtg gccattgtgt tccggacccc tccctacgca
     2161 gaccccagcc tgcaggctcc tgtgcgtgtc tccatgcagc tgcggcggcc ttccgaccgg
     2221 gagctcagtg agcccatgga attccagtac ctgccagata cagacgatcg tcaccggatt
     2281 gaggagaaac gtaaaaggac atatgagacc ttcaagagca tcatgaagaa gagtcctttc
     2341 agcggaccca ccgacccccg gcctccacct cgacgcattg ctgtgccttc ccgcagctca
     2401 gcttctgtcc ccaagccagc accccagccc tatcccttta cgtcatccct gagcaccatc
     2461 aactatgatg agtttcccac catggtgttt ccttctgggc agatcagcca ggcctcggcc
     2521 ttggccccgg cccctcccca agtcctgccc caggctccag cccctgcccc tgctccagcc
     2581 atggtatcag ctctggccca ggccccagcc cctgtcccag tcctagcccc aggccctcct
     2641 caggctgtgg ccccacctgc ccccaagccc acccaggctg gggaaggaac gctgtcagag
     2701 gccctgctgc agctgcagtt tgatgatgaa gacctggggg ccttgcttgg caacagcaca
     2761 gacccagctg tgttcacaga cctggcatcc gtcgacaact ccgagtttca gcagctgctg
     2821 aaccagggca tacctgtggc cccccacaca actgagccca tgctgatgga gtaccctgag
     2881 gctataactc gcctagtgac aggggcccag aggccccccg acccagctcc tgctccactg
     2941 ggggccccgg ggctccccaa tggcctcctt tcaggagatg aagacttctc ctccattgcg
     3001 gacatggact tctcagccct gctgagtcag atcagctcct aagatccacc ggatctagat
     3061 aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3121 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3181 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3241 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     3301 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     3361 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     3421 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     3481 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3541 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3601 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3661 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3721 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg
     3781 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3841 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga
     3901 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3961 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     4021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     4081 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4141 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4201 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca
     4261 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4321 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4381 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4441 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     4501 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4561 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4621 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4681 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4741 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4801 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4861 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4921 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4981 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     5041 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     5101 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     5161 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     5221 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa
     5281 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg
     5341 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
     5401 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
     5461 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct
     5521 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     5581 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     5641 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     5701 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5761 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5821 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5881 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5941 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6001 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6061 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     6121 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     6181 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     6241 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     6301 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     6361 ataaccgtat taccgccatg cat
//
