LOCUS V032294 5703 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V032294 VERSION V032294 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 613..1329 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK" /label="EGFP" CDS 1384..2358 /label="QKI(NM_206854)" /note="QKI(NM_206854)" /gene="QKI" polyA_signal 2491..2612 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2619..3074) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3101..3205 /gene="bla" /label="AmpR promoter" promoter 3207..3564 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3599..4393 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4625..4672 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5001..5589 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgaggc ggcggaggga gtggaggagg gggaagcaag 1381 cttatggtcg gggaaatgga aacgaaggag aagccgaagc ccaccccaga ttacctgatg 1441 cagctgatga acgacaagaa gctcatgagc agcctgccca acttctgcgg gatcttcaac 1501 cacctcgagc ggctgctgga cgaagaaatt agcagagtac ggaaagacat gtacaatgac 1561 acattaaatg gcagtacaga gaaaaggagt gcagaattgc ctgatgctgt gggacctatt 1621 gttcagttac aagagaaact ttatgtgcct gtaaaagaat acccagattt taattttgtt 1681 gggagaatcc ttggacctag aggacttaca gccaaacaac ttgaagcaga aaccggatgt 1741 aaaatcatgg tccgaggcaa aggctcaatg agggataaaa aaaaggagga gcaaaataga 1801 ggcaagccca attgggagca tctaaatgaa gatttacatg tactaatcac tgtggaagat 1861 gctcagaaca gagcagaaat caaattgaag agagcagttg aagaagtgaa gaaattattg 1921 gtacctgcag cagaaggaga agacagcctg aagaagatgc agctgatgga gcttgcgatt 1981 ctgaatggca cctacagaga tgccaacatt aaatcaccag cccttgcctt ttctcttgca 2041 gcaacagccc aggctgctcc aaggatcatt actgggcctg cgccggttct cccaccagct 2101 gccctgcgta ctcctacgcc agctggccct accataatgc ctttgatcag acaaatacag 2161 accgctgtca tgccaaacgg aactcctcac ccaactgctg caatagttcc tccagggccc 2221 gaagctggtt taatctatac accctatgag tacccctaca cattggcacc agctacatca 2281 atccttgagt atcctattga acctagtggt gtattagagt ggattgaaat gccagtcatg 2341 cctgatattt cagcccattg aggatccacc ggatctagat aactgatcat aatcagccat 2401 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 2461 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 2521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 2581 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt 2641 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 2701 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 2761 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 2821 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 2881 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 2941 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 3001 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 3061 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt 3121 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct 3181 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt 3241 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 3301 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3361 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg 3421 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt 3481 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt 3541 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat 3601 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 3661 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 3721 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 3781 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 3841 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 3901 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 3961 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4021 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4081 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg 4141 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4201 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4261 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 4321 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 4381 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 4441 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 4501 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 4561 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct 4621 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc 4681 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa 4741 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg 4801 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt 4861 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 4921 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 4981 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 5041 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 5101 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 5161 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 5221 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 5281 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 5341 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 5401 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 5461 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 5521 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 5581 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 5641 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg 5701 cat //