LOCUS V018441 5906 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V018441 VERSION V018441 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 624..1790 /label="Psmc6(NM_025959)" /note="Psmc6(NM_025959)" /gene="Psmc6" CDS 1797..1826 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /transl_table=1 /translation="EQKLISEEDL" /label="Myc" CDS 1863..2573 /codon_start=1 /note="mammalian codon-optimized" /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /transl_table=1 /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK*" /label="mCherry" polyA_signal 2694..2815 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(2822..3277) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3304..3408 /gene="bla" /label="AmpR promoter" promoter 3410..3767 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3802..4596 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4828..4875 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5204..5792 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagcc accatggcgg accctagaga taaggcgctt caggactatc 661 gcaagaagct gctagagcac aaggagattg acggccgtct taaggagcta agggaacaat 721 taaaagaact taccaagcag tatgaaaagt ctgaaaatga tttgaaggca ctacaaagtg 781 ttggacagat tgtaggtgaa gtgctaaaac agttaacaga agaaaaattc atcgttaaag 841 caacaaatgg accaagatac gttgtaggtt gtcgtcgtca gcttgacaaa agtaagctga 901 agccaggaac cagagttgct ttggatatga ccacactaac catcatgaga tatctgccaa 961 gagaggtgga tccattggtt tataacatgt ctcacgagga tcctggaaat gtatcttatt 1021 ctgagattgg aggcctgtca gaacagattc gggagttaag agaggtaata gaattgcctc 1081 ttacaaatcc agaattattc cagcgtgtag gaataatacc tccaaaaggc tgtttgctct 1141 atggaccgcc aggcactggg aaaacactct tggcacgagc tgttgccagc cagctggact 1201 gcaacttcct aaaggttgta tctagttcta ttgtagacaa gtacattggt gaaagtgctc 1261 gtttgattag agaaatgttt aattatgcca gggaccacca gccatgcatc atttttatgg 1321 atgaaataga tgctattggt ggccgtcggt tttctgaggg aacatcagct gacagagaga 1381 ttcagagaac tttaatggag ttactaaatc aaatggatgg atttgatact ctgcatagag 1441 ttaaaatgat catggctaca aacagaccag atacactgga tcctgctttg ctgcgcccag 1501 gaagattaga tagaaaaata catatcgatt taccaaatga acaagcaaga ttagatatat 1561 tgaaaatcca cgcaggacct attacaaagc atggtgaaat agattatgaa gcaattgtaa 1621 agctttcaga tggctttaat ggagcagacc tgagaaatgt ttgtactgaa gcaggtatgt 1681 ttgcaattcg tgccgatcat gattttgtag ttcaggaaga cttcatgaaa gcagtcagga 1741 aagtggctga ctccaagaag ctggagtcca agctggacta caaacctgtg gaattcgagc 1801 agaagctgat ctcagaggag gacctgacgg taccgcgggc ccgggatcca ccggtcgcca 1861 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca 1921 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 1981 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc 2041 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc 2101 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc 2161 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg 2221 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg 2281 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg 2341 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg 2401 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg 2461 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg 2521 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tagcggccgc 2581 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 2641 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 2701 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 2761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 2821 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 2881 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 2941 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 3001 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 3061 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 3121 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 3181 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 3241 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 3301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 3361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 3421 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 3481 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 3541 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 3601 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3661 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 3721 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 3781 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3841 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3901 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3961 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 4021 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 4081 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 4141 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4201 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4261 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 4321 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 4381 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 4441 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 4501 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4561 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 4621 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 4681 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 4741 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 4801 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 4861 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 4921 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 4981 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 5041 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5101 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5161 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5221 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5281 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5341 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5401 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5461 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5521 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 5581 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 5641 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 5701 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5761 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 5821 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 5881 tggataaccg tattaccgcc atgcat //