LOCUS V034610 6626 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V034610 VERSION V034610 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 625..2535 /label="PRMT5(NM_006109)" /note="PRMT5(NM_006109)" /gene="PRMT5" CDS 2572..3291 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3414..3535 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3542..3997) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4024..4128 /gene="bla" /label="AmpR promoter" promoter 4130..4487 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4522..5316 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5548..5595 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 5924..6512 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgaggc caccatggcg gcgatggcgg tcgggggtgc tggtgggagc 661 cgcgtgtcca gcgggaggga cctgaattgc gtccccgaaa tagctgacac actaggggct 721 gtggccaagc aggggtttga tttcctctgc atgcctgtct tccatccgcg tttcaagagg 781 gagttcattc aggaacctgc taagaatcgg cccggtcccc agacacgatc agacctactg 841 ctgtcaggaa gggactggaa tacgctaatt gtgggaaagc tttctccatg gattcgtcca 901 gactcaaaag tggagaagat tcgcaggaac tccgaggcgg ccatgttaca ggagctgaat 961 tttggtgcat atttgggtct tccagctttc ctgctgcccc ttaatcagga agataacacc 1021 aacctggcca gagttttgac caaccacatc cacactggcc atcactcttc catgttctgg 1081 atgcgggtac ccttggtggc accagaggac ctgagagatg atataattga gaatgcacca 1141 actacacaca cagaggagta cagtggggag gagaaaacgt ggatgtggtg gcacaacttc 1201 cggactttgt gtgactatag taagaggatt gcagtggctc ttgaaattgg ggctgacctc 1261 ccatctaatc atgtcattga tcgctggctt ggggagccca tcaaagcagc cattctcccc 1321 actagcattt tcctgaccaa taagaaggga tttcctgttc tttctaagat gcaccagagg 1381 ctcatcttcc ggctcctcaa gttggaggtg cagttcatca tcacaggcac caaccaccac 1441 tcagagaagg agttctgctc ctacctccaa tacctggaat acttaagcca gaaccgtcct 1501 ccacctaatg cctatgaact ctttgccaag ggctatgaag actatctgca gtccccgctt 1561 cagccactga tggacaatct ggaatctcag acatatgaag tgtttgaaaa ggaccccatc 1621 aaatactctc agtaccagca ggccatctat aaatgtctgc tagaccgagt accagaagag 1681 gagaaggata ccaatgtcca ggtactgatg gtgctgggag caggacgggg acccctggtg 1741 aacgcttccc tgcgggcagc caagcaggcc gaccggcgga taaagctgta tgctgtggag 1801 aaaaacccaa atgccgtggt gacgctagag aactggcagt ttgaagaatg gggaagccaa 1861 gtgaccgtag tctcatcaga catgagggaa tgggtggctc cagagaaagc agacatcatt 1921 gtcagtgagc ttctgggctc atttgctgac aatgaattgt cgcctgagtg cctggatgga 1981 gcccagcact tcctaaaaga tgatggtgtg agcatccccg gggagtacac ttcctttctg 2041 gctcccatct cttcctccaa gctgtacaat gaggtccgag cctgtaggga gaaggaccgt 2101 gaccctgagg cccagtttga gatgccttat gtggtacggc tgcacaactt ccaccagctc 2161 tctgcacccc agccctgttt caccttcagc catcccaaca gagatcctat gattgacaac 2221 aaccgctatt gcaccttgga atttcctgtg gaggtgaaca cagtactaca tggctttgcc 2281 ggctactttg agactgtgct ttatcaggac atcactctga gtatccgtcc agagactcac 2341 tctcctggga tgttctcatg gtttcccatc ctcttcccta ttaagcagcc cataacggta 2401 cgtgaaggcc aaaccatctg tgtgcgtttc tggcgatgca gcaattccaa gaaggtgtgg 2461 tatgagtggg ctgtgacagc accagtctgt tctgctattc ataaccccac aggccgctca 2521 tataccattg gcctcggcgg cggagggagt ggaggagggg gaagcggatc catggtgagc 2581 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 2641 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 2701 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 2761 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 2821 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 2881 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 2941 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 3001 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 3061 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 3121 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 3181 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3241 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc 3301 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 3361 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 3421 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 3481 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 3541 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 3601 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 3661 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 3721 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 3781 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 3841 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 3901 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 3961 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 4021 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 4081 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 4141 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 4201 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 4261 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 4321 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 4381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 4441 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 4501 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 4561 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 4621 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 4681 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 4741 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 4801 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 4861 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4921 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4981 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 5041 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 5101 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 5161 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 5221 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 5281 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 5341 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 5401 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 5461 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 5521 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 5581 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 5641 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 5701 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 5761 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5821 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 6001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 6061 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 6121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 6181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 6241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 6301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 6361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 6421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 6481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 6541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 6601 tggataaccg tattaccgcc atgcat //