LOCUS       V034610                 6626 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034610
VERSION     V034610
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             625..2535
                     /label="PRMT5(NM_006109)"
                     /note="PRMT5(NM_006109)"
                     /gene="PRMT5"
     CDS             2572..3291
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     polyA_signal    3414..3535
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3542..3997)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4024..4128
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        4130..4487
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4522..5316
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5548..5595
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5924..6512
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgaggc caccatggcg gcgatggcgg tcgggggtgc tggtgggagc
      661 cgcgtgtcca gcgggaggga cctgaattgc gtccccgaaa tagctgacac actaggggct
      721 gtggccaagc aggggtttga tttcctctgc atgcctgtct tccatccgcg tttcaagagg
      781 gagttcattc aggaacctgc taagaatcgg cccggtcccc agacacgatc agacctactg
      841 ctgtcaggaa gggactggaa tacgctaatt gtgggaaagc tttctccatg gattcgtcca
      901 gactcaaaag tggagaagat tcgcaggaac tccgaggcgg ccatgttaca ggagctgaat
      961 tttggtgcat atttgggtct tccagctttc ctgctgcccc ttaatcagga agataacacc
     1021 aacctggcca gagttttgac caaccacatc cacactggcc atcactcttc catgttctgg
     1081 atgcgggtac ccttggtggc accagaggac ctgagagatg atataattga gaatgcacca
     1141 actacacaca cagaggagta cagtggggag gagaaaacgt ggatgtggtg gcacaacttc
     1201 cggactttgt gtgactatag taagaggatt gcagtggctc ttgaaattgg ggctgacctc
     1261 ccatctaatc atgtcattga tcgctggctt ggggagccca tcaaagcagc cattctcccc
     1321 actagcattt tcctgaccaa taagaaggga tttcctgttc tttctaagat gcaccagagg
     1381 ctcatcttcc ggctcctcaa gttggaggtg cagttcatca tcacaggcac caaccaccac
     1441 tcagagaagg agttctgctc ctacctccaa tacctggaat acttaagcca gaaccgtcct
     1501 ccacctaatg cctatgaact ctttgccaag ggctatgaag actatctgca gtccccgctt
     1561 cagccactga tggacaatct ggaatctcag acatatgaag tgtttgaaaa ggaccccatc
     1621 aaatactctc agtaccagca ggccatctat aaatgtctgc tagaccgagt accagaagag
     1681 gagaaggata ccaatgtcca ggtactgatg gtgctgggag caggacgggg acccctggtg
     1741 aacgcttccc tgcgggcagc caagcaggcc gaccggcgga taaagctgta tgctgtggag
     1801 aaaaacccaa atgccgtggt gacgctagag aactggcagt ttgaagaatg gggaagccaa
     1861 gtgaccgtag tctcatcaga catgagggaa tgggtggctc cagagaaagc agacatcatt
     1921 gtcagtgagc ttctgggctc atttgctgac aatgaattgt cgcctgagtg cctggatgga
     1981 gcccagcact tcctaaaaga tgatggtgtg agcatccccg gggagtacac ttcctttctg
     2041 gctcccatct cttcctccaa gctgtacaat gaggtccgag cctgtaggga gaaggaccgt
     2101 gaccctgagg cccagtttga gatgccttat gtggtacggc tgcacaactt ccaccagctc
     2161 tctgcacccc agccctgttt caccttcagc catcccaaca gagatcctat gattgacaac
     2221 aaccgctatt gcaccttgga atttcctgtg gaggtgaaca cagtactaca tggctttgcc
     2281 ggctactttg agactgtgct ttatcaggac atcactctga gtatccgtcc agagactcac
     2341 tctcctggga tgttctcatg gtttcccatc ctcttcccta ttaagcagcc cataacggta
     2401 cgtgaaggcc aaaccatctg tgtgcgtttc tggcgatgca gcaattccaa gaaggtgtgg
     2461 tatgagtggg ctgtgacagc accagtctgt tctgctattc ataaccccac aggccgctca
     2521 tataccattg gcctcggcgg cggagggagt ggaggagggg gaagcggatc catggtgagc
     2581 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2641 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2701 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2761 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2821 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2881 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2941 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3001 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3061 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3121 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3181 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3241 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc
     3301 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3361 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3421 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3481 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg
     3541 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3601 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3661 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3721 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3781 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3841 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3901 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3961 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     4021 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4081 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
     4141 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4201 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4261 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4321 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4441 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     4501 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     4561 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     4621 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     4681 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     4741 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     4801 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     4861 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     4921 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     4981 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5041 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     5101 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     5161 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     5221 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     5281 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     5341 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     5401 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     5461 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     5521 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     5581 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     5641 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     5701 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     5761 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5821 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5881 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5941 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6001 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6061 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6121 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6181 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6241 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6301 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6361 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6421 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6481 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6541 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     6601 tggataaccg tattaccgcc atgcat
//
