LOCUS       V018377                 6105 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018377
VERSION     V018377
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        61..364
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        365..568
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             613..1329
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1366..2760
                     /label="PRKN(NM_004562)"
                     /note="PRKN(NM_004562)"
                     /gene="PRKN"
     polyA_signal    2893..3014
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(3021..3476)
                     /direction=LEFT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3503..3607
                     /gene="bla"
                     /label="AmpR promoter"
     promoter        3609..3966
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4001..4795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5027..5074
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal&nbsp;(Cole and Stacy, 1985)"
                     /label="HSV TK poly(A) signal"
     rep_origin      5403..5991
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcgatgat agtgtttgtc
     1381 aggttcaact ccagccatgg tttcccagtg gaggtcgatt ctgacaccag catcttccag
     1441 ctcaaggagg tggttgctaa gcgacagggg gttccggctg accagttgcg tgtgattttc
     1501 gcagggaagg agctgaggaa tgactggact gtgcagaatt gtgacctgga tcagcagagc
     1561 attgttcaca ttgtgcagag accgtggaga aaaggtcaag aaatgaatgc aactggaggc
     1621 gacgacccca gaaacgcggc gggaggctgt gagcgggagc cccagagctt gactcgggtg
     1681 gacctcagca gctcagtcct cccaggagac tctgtggggc tggctgtcat tctgcacact
     1741 gacagcagga aggactcacc accagctgga agtccagcag gtagatcaat ctacaacagc
     1801 ttttatgtgt attgcaaagg cccctgtcaa agagtgcagc cgggaaaact cagggtacag
     1861 tgcagcacct gcaggcaggc aacgctcacc ttgacccagg gtccatcttg ctgggatgat
     1921 gttttaattc caaaccggat gagtggtgaa tgccaatccc cacactgccc tgggactagt
     1981 gcagaatttt tctttaaatg tggagcacac cccacctctg acaaggaaac atcagtagct
     2041 ttgcacctga tcgcaacaaa tagtcggaac atcacttgca ttacgtgcac agacgtcagg
     2101 agccccgtcc tggttttcca gtgcaactcc cgccacgtga tttgcttaga ctgtttccac
     2161 ttatactgtg tgacaagact caatgatcgg cagtttgttc acgaccctca acttggctac
     2221 tccctgcctt gtgtggctgg ctgtcccaac tccttgatta aagagctcca tcacttcagg
     2281 attctgggag aagagcagta caaccggtac cagcagtatg gtgcagagga gtgtgtcctg
     2341 cagatggggg gcgtgttatg cccccgccct ggctgtggag cggggctgct gccggagcct
     2401 gaccagagga aagtcacctg cgaagggggc aatggcctgg gctgtgggtt tgccttctgc
     2461 cgggaatgta aagaagcgta ccatgaaggg gagtgcagtg ccgtatttga agcctcagga
     2521 acaactactc aggcctacag agtcgatgaa agagccgccg agcaggctcg ttgggaagca
     2581 gcctccaaag aaaccatcaa gaaaaccacc aagccctgtc cccgctgcca tgtaccagtg
     2641 gaaaaaaatg gaggctgcat gcacatgaag tgtccgcagc cccagtgcag gctcgagtgg
     2701 tgctggaact gtggctgcga gtggaaccgc gtctgcatgg gggaccactg gttcgacgtg
     2761 tagggatcca ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     2821 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     2881 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2941 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3001 atcaatgtat cttaacgcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3061 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3121 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3181 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3241 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3301 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3361 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3421 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     3481 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3541 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3601 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     3661 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3721 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3781 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3841 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3901 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3961 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4021 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4081 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4141 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     4201 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4261 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4321 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4381 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4441 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4501 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     4561 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     4621 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     4681 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     4741 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     4801 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     4861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     4921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     4981 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     5041 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5101 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     5161 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     5221 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     5281 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5341 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5401 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5461 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5521 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     5581 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5641 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5701 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5761 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5821 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5881 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5941 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6001 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6061 gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcat
//
